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We narrowed to 13 results for: LARGE

Showing: 1 - 13 of 13 results

  1. CRISPR Guide

    Type
    Guide
    ... Browse Plasmids: Prime Edit Large Scale Edits CRISPR can generate large-scale genetic changes, including...CRISPR Guide Overview CRISPR Basics Small Edits Large Edits Genome-Wide Screening Cas9 Fusion Tools Cas9...knockout or gene edit; or using Cas9 to generate large genomic deletions or inversions with two gRNA sites...plasmid or linear dsDNA templates work better for large edits or insertions. When designing the donor template...increase HDR efficiency. In most applications, a large portion of the Cas9-induced DSBs will still be repaired...Transposon systems like piggyBac or Tn7 allow for large scale edits by integrating segments of DNA flanked..., developed by the Feng Zhang lab ) combine the large-scale capacity of transposases with the precise ...

  2. Guide to Using Pooled Libraries

    Type
    Guide
    ...designed to cover only a subset of genes, or very large (e.g. the Toronto KnockOut (TKO) library from the... of cells as plasmids in the TKO library. Using large numbers of cells minimizes the effects of random... CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens New Tool for Lineage Tracing...

  3. Plan Your Experiment

    Type
    Guide
    ...edits, including point mutations, base changes, and large insertions or deletions. For more information on...efficiencies. The template sequences can also have a large impact on editing efficiency. So while testing multiple...of AAV vectors is that the packaging limit (how large of a DNA sequence you can actually package into ...edit. Even if you provided a homology template, a large percentage of DNA breaks will be repaired by NHEJ... Can't distinguish specific edits PCR Knockouts Large insertions/deletions Some prime and base edits Can...inefficient methods and often require screening a larger pool of cells to find one with your desired edit...

  4. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...activates the necessary cellular machinery to produce large amounts of rAAV vectors. The E1 gene is not included...distributing widely throughout the organism to target large or diffused biological structures. Specifically,...visualize connections between neurons within the brain. Large-scale examples of mapping the brain through neuronal... capacity Another strategy to deliver cargo too large to fit within the rAAV capsid, such as Cas9-derived...which has shown higher efficiency in delivering large genes makes use of split inteins, proteins that ...be expressed. This method allows for delivery of larger transgenes, but expression is still much less efficient...

  5. Lentiviral Vector Guide

    Type
    Guide
    ...First-generation First-generation lentiviral plasmids are largely defunct due to their lack of safety improvements...on different plasmids, the viral genome remains largely intact. First-generation plasmids include (Figure...full-length virus. The potential for oncogenesis is largely based on whether or not the specific insert contained...identifies alterations in chromatin architecture through large-scale mapping of lentiviral integration sites. Nature...

  6. Adenovirus Guide

    Type
    Guide
    ...vectors, rAdV vectors can provide a large transgene packaging capacity. Large protein-coding genes and complex... and third-generation vectors. This capacity is large enough to carry the Cas9 gene and a gRNA expression...shorter period of time or when needing to package larger cargo. CRISPR-Cas9 Delivery As compared to other...backbone plasmid that lacks E1, E3, and E4. Useful for larger inserts. pAdTrack Class of shuttle/transfer vectors...

  7. Gamma-Retroviral Vector Guide

    Type
    Guide
    ... protocols for fast and efficient generation of large-scale, high-titer preps. This is thanks to established...full-length virus. The potential for oncogenesis is largely based on whether or not the specific insert contained...

  8. Antibody Guide

    Type
    Guide
    ...above are used to create hybridomas, which produce large amounts of a single antibody with little variation...antibody is made up of four protein chains: two large heavy chains and two small light chains. These four... fluorophore colors in flow cytometry, but the largest panels used currently are in the mid-forties. When...

  9. Modular Cloning Guide

    Type
    Guide
    ...Christopher A. O'Callaghan Toolkit for assembling large DNA fragments based on methylation-switching. 4G-Cloning...genetic engineering of S. cerevisiae , enabling larger multi-gene constructs, multiplexed CRISPR editing...

  10. Optogenetics Guide

    Type
    Guide
    ...function by reversibly sequestering targets into large, optically assembled protein architectures in living...(CrChR2) 470 ChR2/H134 Widely used variant with larger photocurrent compared to CrChR2 450 ChETA E123T...

  11. Molecular Biology Reference

    Type
    Guide
    ...(mammalian, yeast, bacterial, etc.), depending largely on which promoter is used to initiate transcription...

  12. Cloning

    Type
    Guide
    ...overlapping homology. One major advantage is that much larger final products can be generated (up to 100kb) compared... progressively more difficult to clone plasmids larger than 10kb. Another advantage is the ability to ...

  13. Chemogenetics Guide

    Type
    Guide
    ... on G-protein coupled receptors, or GPCRs. The largest class of cell surface receptors, GPCRs are seven-pass...
Showing: 1 - 13 of 13 results