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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 23 results for: LARGE

Showing: 1 - 20 of 23 results

  1. Protocol - How to Inoculate a Bacterial Culture

    Type
    Protocol
    ...individual plasmid within a single bacterial cell. Large plasmids usually have a low copy number (approximately... other hand, smaller plasmids can be present in large numbers, 50 or more per cell and have a high copy...usually want to start 2 mL in a falcon tube, but for larger preps you might want to use as much as a liter ...

  3. Protocol - Bacterial Transformation

    Type
    Protocol
    ...less efficient at taking up larger plasmids. If you need to transform large plasmids, it is a good idea...very few if any transformants when transforming large plasmids (>10 kb) or BACs, what can I do? Chemically...

  7. Pipetting Protocol

    Type
    Protocol
    ... liquid. Pro-Tip If pipetting from a relatively large container holding a small volume of liquid, tilt...

  12. Pouring LB Agar Plates

    Type
    Protocol
    ... you will be screening a large number of colonies, we recommend using larger plates. Many labs use 100...

  13. AAV Production in HEK293 Cells

    Type
    Protocol
    ...efficient means to scale-up without having to handle a large number of T-175 flasks. Workflow Timeline Day 0:... (PES) filter system, Nalgene, 565-0010 (or for larger volumes 1000 mL 0.45 µm Rapid-Flow PES Filtration...

  14. Centrifugation

    Type
    Protocol
    ...microfuge tubes used in labs, though some larger models can hold larger conical tubes. Depending on the centrifuge...speeds to their smaller cousins, but can hold much larger containers. Ultracentrifuges are also generally...generally floor sized centrifuges that can hold larger containers, but they can also rotate at much higher speeds...

  15. Using a Light Microscope Protocol

    Type
    Protocol
    ...are magnification (the ability to make an image larger) and resolution (the ability to distinguish between...diaphragm and use the coarse focus adjustment knob (the larger of the two focus knobs) until you can see the edges...magnification, you will notice that the objectives get larger. Be careful to avoid hitting your sample with the...magnification, remember that the objectives are larger. Adjust your focus slowly so that you don't accidentally...

  17. Weighing Reagents Protocol

    Type
    Protocol
    ...places. Pro-Tip If you’re weighing out an amount larger than the capacity of your balance or weighing boat...
Showing: 1 - 20 of 23 results