We narrowed to 2,482 results for: PRK

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pRK5_tAKARζ

Plasmid

#119918

Purpose

Mammalian expression of the tAKARζ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

Depositor

Haining Zhong

Article

Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

Insert

tAKARζ

Expression

Mammalian

Available Since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pFRT_TO_FLAGHA_PRKRA_sgRNA_PAM_mutant

Plasmid

#106112

Purpose

Inducible gene expression of FlagHA-PRKRA sgRNA PAM mutant

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

PRKRA (PRKRA Human)

Tags

Flag and HA

Expression

Mammalian

Mutation

silent mutation of Gly32 (GGG-->GGT)

Available Since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pRK084

Plasmid

#250141

Purpose

Expression of truncated PGL-3 in E. coli

Depositor

Geraldine Seydoux

Article

Kuroiwa et al EMBO Rep. 2026 Mar 20. doi: 10.1038/s443

Insert

PGL-3(D1-D2-3(GGGGS)-RGG) (pgl-3 Nematode)

Tags

6xHis::MBP::6xHis::TEV cleavage site

Expression

Bacterial

Available Since

March 31, 2026

Availability

Academic Institutions and Nonprofits only

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pRK047

Plasmid

#250145

Purpose

Expression of mutant PGL-3 in E. coli

Depositor

Geraldine Seydoux

Article

Kuroiwa et al EMBO Rep. 2026 Mar 20. doi: 10.1038/s443

Insert

PGL-3(FL)(R123E, K126E, K129E) (pgl-3 Nematode)

Tags

6xHis::MBP::6xHis::TEV cleavage site

Expression

Bacterial

Mutation

R123E, K126E, K129E

Available Since

March 31, 2026

Availability

Academic Institutions and Nonprofits only

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pRK015

Plasmid

#250134

Purpose

Expression of full-length PGL-3 in E. coli

Depositor

Geraldine Seydoux

Article

Kuroiwa et al EMBO Rep. 2026 Mar 20. doi: 10.1038/s443

Insert

PGL-3(FL) (pgl-3 Nematode)

Tags

6xHis::MBP::6xHis::TEV cleavage site

Expression

Bacterial

Available Since

March 31, 2026

Availability

Academic Institutions and Nonprofits only

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pRK049

Plasmid

#250135

Purpose

Expression of truncated PGL-3 in E. coli

Depositor

Geraldine Seydoux

Article

Kuroiwa et al EMBO Rep. 2026 Mar 20. doi: 10.1038/s443

Insert

PGL-3(D1) (pgl-3 Nematode)

Tags

6xHis::MBP::6xHis::TEV cleavage site

Expression

Bacterial

Available Since

March 31, 2026

Availability

Academic Institutions and Nonprofits only

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pRK050

Plasmid

#250136

Purpose

Expression of truncated PGL-3 in E. coli

Depositor

Geraldine Seydoux

Article

Kuroiwa et al EMBO Rep. 2026 Mar 20. doi: 10.1038/s443

Insert

PGL-3(D2) (pgl-3 Nematode)

Tags

6xHis::MBP::6xHis::TEV cleavage site

Expression

Bacterial

Available Since

March 31, 2026

Availability

Academic Institutions and Nonprofits only

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pRK011

Plasmid

#250137

Purpose

Expression of truncated PGL-3 in E. coli

Depositor

Geraldine Seydoux

Article

Kuroiwa et al EMBO Rep. 2026 Mar 20. doi: 10.1038/s443

Insert

PGL-3(IDR) (pgl-3 Nematode)

Tags

MBP::6xHis::TEV cleavage site

Expression

Bacterial

Available Since

March 31, 2026

Availability

Academic Institutions and Nonprofits only

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pRK036

Plasmid

#250138

Purpose

Expression of truncated PGL-3 in E. coli

Depositor

Geraldine Seydoux

Article

Kuroiwa et al EMBO Rep. 2026 Mar 20. doi: 10.1038/s443

Insert

PGL-3(D1-D2) (pgl-3 Nematode)

Tags

6xHis::MBP::6xHis::TEV cleavage site

Expression

Bacterial

Available Since

March 31, 2026

Availability

Academic Institutions and Nonprofits only

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pRK042

Plasmid

#250139

Purpose

Expression of truncated PGL-3 in E. coli

Depositor

Geraldine Seydoux

Article

Kuroiwa et al EMBO Rep. 2026 Mar 20. doi: 10.1038/s443

Insert

PGL-3(D1-D2-IDR) (pgl-3 Nematode)

Tags

6xHis::MBP::6xHis::TEV cleavage site

Expression

Bacterial

Available Since

March 31, 2026

Availability

Academic Institutions and Nonprofits only

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pRK082

Plasmid

#250140

Purpose

Expression of truncated PGL-3 in E. coli

Depositor

Geraldine Seydoux

Article

Kuroiwa et al EMBO Rep. 2026 Mar 20. doi: 10.1038/s443

Insert

PGL-3(D1-D2-RGG) (pgl-3 Nematode)

Tags

6xHis::MBP::6xHis::TEV cleavage site

Expression

Bacterial

Available Since

March 31, 2026

Availability

Academic Institutions and Nonprofits only

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TEVESNp_pRK793

Plasmid

#247042

Purpose

pRK793 (from David Waugh, Addgene plasmid #8827) backbone with ENLYFES cut site and TEVESNp insert.

Depositor

Carl Denard

Article

Nelson et al ACS Synth Biol. 2025 Jun 20;14(6):2241-2

Insert

MBP-ENLYFES-7xHIS-TEVESNp

Tags

His and MBP

Expression

Bacterial

Mutation

S120C, D148R, Tl73A, N174R, N176 V, Nl77K, G213A,…

Available Since

Feb. 18, 2026

Availability

Academic Institutions and Nonprofits only

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TEVEp_pRK793

Plasmid

#247672

Purpose

pRK793 (from David Waugh, Addgene plasmid #8827) backbone with ENLYFES cut site and TEVEp insert based on Yi et al., 2013.

Depositor

Carl Denard

Article

Nelson et al ACS Synth Biol. 2025 Jun 20;14(6):2241-2

Insert

MBP-ENLYFES-7xHIS-TEVEp

Tags

His and MBP

Expression

Bacterial

Mutation

S120R, D148R, T173A, N177K, M218I, S219P

Available Since

Feb. 18, 2026

Availability

Academic Institutions and Nonprofits only

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pRK5_V5-nb-KS

Plasmid

#238266

Purpose

For overexpression of V5-nb-KS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

V5-nb-KS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mutCKAR3

Plasmid

#238412

Purpose

Mammalian expression of a phosphorylation deficient (T to A) mutant of CKAR3 under a CMV promotor.

Depositor

Haining Zhong

Article

Yahiro et al bioRxiv [Preprint]. 2024 Jul 23:2024.07.

Insert

phosphorylation deficient mutant of CKAR3 (T to A)

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_PRKCE_AFTPH

Plasmid

#205901

Purpose

Express mEGFP-tagged fusion protein, PRKCE_AFTPH from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

PRKCE_AFTPH (PRKCE, AFTPH Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pRK011

Plasmid

#69875

Purpose

expression of Channelrhodopsin-2 T159C mutant

Depositor

Robert Kittel, Georg Nagel

Article

Dawydow et al Proc Natl Acad Sci U S A. 2014 Sep 23;11

Insert

Channelrhodopsin-2

Use

Unspecified

Mutation

T159C

Promoter

Drosophila Hsp70

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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PRKCG_Halo_C_allele

Plasmid

#178146

Purpose

Donor vector for endogenous tagging of human PRKCG at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (PRKCG Human)

Use

Donor vector

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pRK5_tAKARδ

Plasmid

#119916

Purpose

Mammalian expression of the tAKARδ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

Depositor

Haining Zhong

Article

Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

Insert

tAKARδ

Expression

Mammalian

Availability

Academic Institutions and Nonprofits only

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pRK5_tAKARε

Plasmid

#119917

Purpose

Mammalian expression of the tAKARε sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

Depositor

Haining Zhong

Article

Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

Insert

tAKARε

Expression

Mammalian

Availability

Academic Institutions and Nonprofits only

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