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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,169 results for: pFAST

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Showing: 61 - 80 of 1169 results

  1. FLAGHis10pFastBacHTA/Serca1

    Plasmid
    #74401
    Purpose
    expression of SERCA in insect cells
    Depositor
    Robert Nakamoto
    Insert
    SERCA1 (ATP2A1 rabbit)
    Tags
    10His and FLAG
    Expression
    Insect
    Mutation
    NcoI site destroyed (silent) T898
    Promoter
    polyhedrin
    Available Since
    April 6, 2016
    Availability
    Academic Institutions and Nonprofits only

  10. pFastBac1-C17orf53

    Plasmid
    #211445
    Purpose
    generation of Bacmid for insect cell expression of C17orf53
    Depositor
    Helmut Pospiech
    Insert
    C17orf53 (HROB Human)
    Expression
    Insect
    Available Since
    Jan. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    211445-plasmid-map-sequence-id-419422

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  11. Plekhm1 pFastHFTb

    Plasmid
    #73827
    Purpose
    Express human Plekhm1 in insect cells
    Depositor
    Paul Odgren
    Insert
    Plekhm1 (PLEKHM1 Human)
    Tags
    6xHis and Flag
    Expression
    Insect
    Available Since
    March 15, 2016
    Availability
    Academic Institutions and Nonprofits only

  14. pFastBac1-pGC-A

    Plasmid
    #186626
    Purpose
    pFastBac1 (baculovirus polyhedrin promoter) encoding HA signal peptide, TEV-protease cleavable His10-tag, and full-length human pGC-A (NP_000897.3 amino acids 33-1061; expression-optimized DNA)
    Depositor
    Debra Hansen
    Article
    Zhang et al Sci Rep. 2022 Jul 12;12(1):11824. doi: 1
    Insert
    NPR1 (NPR1 Human)
    Tags
    hemagglutinin (HA) signal peptide + His10-tag + T…
    Expression
    Insect
    Mutation
    deleted amino acids 1-32
    Promoter
    polyhedring
    Available Since
    July 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    186626-plasmid-map-sequence-id-366906

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  16. pFastBac1-His10FLAG

    Plasmid
    #157736
    Purpose
    empty pFastBac1 vector (polyhedrin promoter) adds enterokinase-removable N-terminal His10-tag + 3xFLAG tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sites
    Depositor
    Debra Hansen
    Article
    Olson et al Protein Expr Purif. 2021 May 7:105890. d
    Type
    Empty backbone
    Tags
    His10 + 3xFLAG, enterokinase-cleavable
    Expression
    Insect
    Promoter
    polyhedrin
    Available Since
    June 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    157736-plasmid-map-sequence-id-333926

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  19. pFastBac HT JS-Munc18c

    Plasmid
    #135555
    Purpose
    Express mouse Munc18c in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Munc18c.
    Depositor
    Jingshi Shen
    Article
    Yu et al Methods Mol Biol. 2019;1860:237-249. doi
    Insert
    Munc18c
    Tags
    6xHis tag and TEV site
    Expression
    Insect
    Promoter
    polyhedrin promoter
    Available Since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    135555-plasmid-map-sequence-id-272498

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Showing: 61 - 80 of 1169 results