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pFOSGE_COF2E10

Plasmid

#70929

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Elp2 (Elp2 Fly)

Use

Gateway entry vector

Available Since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF2G03

Plasmid

#70882

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

TfIIFalpha (TfIIFalpha Fly)

Use

Gateway entry vector

Available Since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF2D05

Plasmid

#70892

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

dbo (dbo Fly)

Use

Gateway entry vector

Available Since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF2G06

Plasmid

#70900

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

MED17 (MED17 Fly)

Use

Gateway entry vector

Available Since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF2H07

Plasmid

#70908

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

CG7179 (CG7179 Fly)

Use

Gateway entry vector

Available Since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF2H09

Plasmid

#70924

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Mnn1 (Mnn1 Fly)

Use

Gateway entry vector

Available Since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3-5'UTR-MAVS-ORF3,4(KaganE50)

Plasmid

#52007

Purpose

Expresses the human MAVS CDS and contains the 5'UTR sequence with a mutation in the start codons 61 and 67 nt downstream of the FL MAVS start codon

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

5utr-MAVS-ORF2,3mut

Expression

Mammalian

Mutation

mutated start codons for ORF3 and 4

Available Since

April 14, 2014

Availability

Academic Institutions and Nonprofits only
7a sgRNA for EJ7-GFP and 4-μHOM reporters

Plasmid

#113620

Purpose

sgRNA/CAS9 expression plasmid to induce the 5’ double-strand break in both the EJ7-GFP and 4-μHOM reporters

Depositor

Jeremy Stark

Article

Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

Insert

7a sgRNA

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only
7b sgRNA for EJ7-GFP reporter

Plasmid

#113624

Purpose

sgRNA/CAS9 expression plasmid to induce the 3’ double-strand break in the EJ7-GFP reporter

Depositor

Jeremy Stark

Article

Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

Insert

7b sgRNA

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only
7i sgRNA for 4-μHOM reporter

Plasmid

#113626

Purpose

sgRNA/CAS9 expression plasmid to induce a 3’ double-strand break in the 4-μHOM reporter 8 nts. upstream from the microhomology

Depositor

Jeremy Stark

Article

Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

Insert

7i sgRNA

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only
pJL1-CRM197-4xDQNAT

Plasmid

#128395

Purpose

In vitro expression of CRM197 with C terminal 4xDQNAT glycosylation sites

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Insert

Diphtheria toxin

Tags

4xDQNAT glycosylation tag and 6xHis tag

Expression

Bacterial

Mutation

Inactivating G52E mutation

Promoter

T7

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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7h sgRNA for 4-μHOM reporter

Plasmid

#113625

Purpose

sgRNA/CAS9 expression plasmid to induce a 3’ double-strand break in the 4-μHOM reporter at the edge of the microhomology.

Depositor

Jeremy Stark

Article

Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

Insert

7h sgRNA

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only
pCAGGS-I-SceI-Trex2

Plasmid

#44024

Depositor

Jeremy Stark

Article

Gunn et al J Biol Chem. 2011 Dec 9;286(49):42470-82

Insert

I-SceI fused to mTREX2

Tags

NLS-HA

Expression

Mammalian

Promoter

pCAGGS

Available Since

April 24, 2013

Availability

Academic Institutions and Nonprofits only

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pSF-CjPglB-LpxE

Plasmid

#128389

Purpose

Expression of C. jejuni PglB and F. tularensis LpxE in E. coli

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Inserts

PglB oligosaccharylktransferase
LpxE phosphatase

Tags

Flag tag

Expression

Bacterial

Promoter

Ptac and pBAD

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pJL1-PD-4xDQNAT

Plasmid

#128391

Purpose

In vitro expression of H. influenzae protein D with C terminal 4xDQNAT glycosylation sites

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Insert

Protein D

Tags

4xDQNAT glycosylation tag and 6xHis tag

Expression

Bacterial

Promoter

T7

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF4A02

Plasmid

#71021

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Ube3a (Ube3a Fly)

Use

Gateway entry vector

Available Since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only
pGL3_4xUAS_CP-candidate_luc+

Plasmid

#125154

Purpose

STAP-seq luciferase validation vector, which harbors an 4x UAS array upstream of the CP position (candidates are inserted at CP position) for the recruitement of GAL4-DBD tagged proteins

Depositor

Alexander Stark

Article

Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

Insert

4 x upstream activating sequence (UAS)

Use

Luciferase

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pCoofy62

Plasmid

#122014

Purpose

Mammalian vector for parallel SLIC cloning containing the VEGF signal sequence for secretion, N-terminal His6-Smt3Star (SumoStar*) and different C-tag options

Depositor

Sabine Suppmann

Article

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Tags

His6-Smt3Star and Multi3: depending on the LP2 pr…

Expression

Mammalian

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pJL1-PorA-4xDQNAT

Plasmid

#128392

Purpose

In vitro expression of N. meningitidis PorA with C terminal 4xDQNAT glycosylation sites

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Insert

PorA

Tags

4xDQNAT glycosylation tag and 6xHis tag

Expression

Bacterial

Promoter

T7

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pJL1-TTc-4xDQNAT

Plasmid

#128393

Purpose

In vitro expression of tetanus toxin fragment C with C terminal 4xDQNAT glycosylation sites

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Insert

Tetanus toxin fragment C domain

Tags

4xDQNAT glycosylation tag and 6xHis tag

Expression

Bacterial

Promoter

T7

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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