We narrowed to 2,690 results for: T2A

1. pBS-KS-attB2-SA(1)-T2A-Gal4DBD-Hsp70

   Plasmid

   #62903

   Purpose

   Creating Gal4DBD hemidrivers from MiMIC lines with inserts into coding introns in Phase 1

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Insert

   T2A-Gal4DBD-Hsp70
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. AAV2-miniSOG-VAMP2-T2A-mCherry

   Plasmid

   #50970

   Purpose

   AAV2 transfer vector containing the InSynC (miniSOG-VAMP2-T2A-mCherry) construct

   Depositor

   Roger Tsien

   Article

   Lin et al Neuron. 2013 Jul 24;79(2):241-53. doi: 1

   Insert

   miniSOG-VAMP2-T2A-mCherry (Vamp2 Mouse, Arabdopsis thaliana)
   

   Use

   AAV

   Tags

   T2A-mCherry and miniSOG

   Expression

   Mutation

   Promoter

   human synapsin promoter

   Available since

   Feb. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSBbi-moxGFP-P2A-T2A-Puro

   Plasmid

   #176893

   Purpose

   Sleeping Beauty transposon-based vector for mammalian polycistronic expression of moxGFP and two additional genes of interest

   Depositor

   Jorge Luis Arias-Arias

   Article

   Acon et al iScience. 2021 Nov 8;24(12):103407. doi:

   Insert

   moxGFP-P2A-T2A
   

   Use

   Transposon

   Tags

   moxGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a/RPBSA

   Available since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBS-KS-attB2-SA(2)-T2A-p65AD-Hsp70

   Plasmid

   #62915

   Purpose

   Creating p65AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 2

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Insert

   T2A-p65AD-Hsp70
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pC-(lox2-attB2-SA-T2A-Gal4-Hsp70)3

   Plasmid

   #62957

   Purpose

   Triplet donor for in vivo RMCE with MiMIC inserts to make Gal4 driver.

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Insert

   T2A-Gal4 in three phases
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. P-Ef1a-EGFP-T2A-RPL22-1xFlag

   Plasmid

   #170323

   Purpose

   Expresses FLAG and EGFP tagged RPL22

   Depositor

   Hemali Phatnani

   Article

   Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

   Insert

   RPL22 (RPL22 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBS-KS-attB2-SA(2)-T2A-Gal4-Hsp70

   Plasmid

   #62898

   Purpose

   Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 2.

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Insert

   T2A-Gal4-Hsp70
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCRISPaint-T2A-sfGFP-3xP3-RFP

   Plasmid

   #127557

   Purpose

   CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-sfGFP-SV40 and 3xP3-RFP visible eye marker.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

   Insert

   sfGFP
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCRISPaint-T2A-FLPo-3xP3-RFP

   Plasmid

   #127561

   Purpose

   CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-FLPo-SV40 and 3xP3-RFP visible eye marker.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

   Insert

   FLPo
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pU6-SCGB3A2_gRNA1-SpCas9-T2A-GFP

    Plasmid

    #126698

    Purpose

    to express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human SCGB3A2 locus at the end of the endogenous coding sequence

    Depositor

    Darrell Kotton

    Article

    McCauley et al Stem Cell Reports. 2018 May 8;10(5):1579

    Insert

    sgRNA targeting human SCGB3A2 locus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBS-KS-attB2-SA(0)-T2A-Gal4DBD-Hsp70

    Plasmid

    #62902

    Purpose

    Creating Gal4DBD hemidrivers from MiMIC lines with inserts into coding introns in Phase 0

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-Gal4DBD-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Pdgfra-T2A-H2B-eGFP donor

    Plasmid

    #113120

    Purpose

    Donor plasmid for knock-in T2A-H2B-eGFP to the c-terminal of mouse Pdgfra coding sequence

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    Pdgfra donor region including homology arms and T2A-H2B-eGFP (Pdgfra Mouse, Synthetic)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCDH-hSyn-NCreIntN-T2A-mTagBFP2

    Plasmid

    #160502

    Purpose

    Expresses NCreIntN in neurons in split intein-mediated split-Cre recombinase applications

    Depositor

    Shawn Je

    Article

    Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

    Insert

    NCreIntN
    

    Use

    Cre/Lox and Lentiviral

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGEMT-PT2A-iRFP670-Tdtomato-GFP

    Plasmid

    #111817

    Purpose

    Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

    Depositor

    Li Qian

    Article

    Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

    Inserts

    iRFP670
    Tdtomato
    GFP
    

    Use

    Cloning intermediate

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pU6-cGAS g3 Cas9-T2A-mCherry

    Plasmid

    #164254

    Purpose

    Transcription of cGAS guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

    Depositor

    John Maciejowski

    Article

    Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

    Insert

    sgcGAS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCRISPaint-T2A-Gal80-3xP3-RFP

    Plasmid

    #127562

    Purpose

    CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal80-SV40 and 3xP3-RFP visible eye marker.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    Gal80
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV-EGFP-T2A-ACREB-WPRE

    Plasmid

    #40867

    Depositor

    Harold Gainer

    Article

    Lubelski et al Brain Res. 2012 Jan 6;1429:18-28. Epub 2

    Insert

    ACREB
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Nov. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV hSyn1 CaRhGC wt T2A tDimer

    Plasmid

    #101720

    Purpose

    humanized, Neuron-specific promoter driven expression of the rhodopsin guanylyl cyclase from Catenaria anguillulae with a neuron-specific promoter. Useful for raising intracellular cAMP close to the m

    Depositor

    Thomas Oertner

    Article

    Scheib et al Nat Commun. 2018 May 24;9(1):2046. doi:

    Inserts

    CatRhGC
    red fluorescent protein
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn1 and ribosomal skip sequence T2A

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAc5 HA3-eDHFR-T2A-neo

    Plasmid

    #86396

    Purpose

    for C-terminal tagging with eDHFR trimethoprim regulated degron with neo selection

    Depositor

    David Bentley

    Article

    Sheridan et al Biotechniques. 2016 Feb 1;60(2):69-74. d

    Insert

    HA3 eDHFR T2A neo
    

    Use

    CRISPR

    Tags

    HA3 eDHFR T2A neo

    Expression

    Mutation

    R12Y, G67S and Y100I mutations in E coli DHFR

    Promoter

    Available since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDH-CAG-NCreIntN-T2A-mTagBFP2

    Plasmid

    #160500

    Purpose

    Expresses NCreIntN in mammalian cells in split intein-mediated split-Cre recombinase applications

    Depositor

    Shawn Je

    Article

    Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

    Insert

    NCreIntN
    

    Use

    Cre/Lox and Lentiviral

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge