We narrowed to 3,816 results for: golden gate

1. pLP3

   Plasmid

   #209958

   Purpose

   Entry vector to store Level 0 broad-host range L. plantarum replication origin parts

   Depositor

   Thorsten Mascher

   Article

   Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Sept. 13, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pLPP1

   Plasmid

   #209963

   Purpose

   Contains Level 0 Part: mock / negative control Promoter (P-nc) for the construction of Level 1 plasmids

   Depositor

   Thorsten Mascher

   Article

   Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

   Insert

   Promoter (mock)
   

   Expression

   Bacterial

   Available Since

   Sept. 13, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pLPP2

   Plasmid

   #209964

   Purpose

   Contains Level 0 Part: constitutive Promoter (P48) for the construction of Level 1 plasmids

   Depositor

   Thorsten Mascher

   Article

   Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

   Insert

   Promoter (P48)
   

   Expression

   Bacterial

   Available Since

   Sept. 13, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pLPC31

   Plasmid

   #209996

   Purpose

   Contains Level 0 Part: 3' Connector (3C2SN) for the construction of Level 1 plasmids

   Depositor

   Thorsten Mascher, Georg Fritz

   Article

   Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

   Insert

   3' Connector (3C2SN)
   

   Expression

   Bacterial

   Available Since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLPC32

   Plasmid

   #209997

   Purpose

   Contains Level 0 Part: 3' Connector (3C5CSN) for the construction of Level 1 plasmids

   Depositor

   Thorsten Mascher, Georg Fritz

   Article

   Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

   Insert

   3' Connector (3C5CSN)
   

   Expression

   Bacterial

   Available Since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLPC52

   Plasmid

   #209991

   Purpose

   Contains Level 0 Part: 5' Connector (5C2SN) for the construction of Level 1 plasmids

   Depositor

   Thorsten Mascher, Georg Fritz

   Article

   Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

   Insert

   5' Connector (5C2SN)
   

   Expression

   Bacterial

   Available Since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLPC51

   Plasmid

   #209990

   Purpose

   Contains Level 0 Part: 5' Connector (5C1CSN) for the construction of Level 1 plasmids

   Depositor

   Thorsten Mascher, Georg Fritz

   Article

   Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

   Insert

   5' Connector (5C1CSN)
   

   Expression

   Bacterial

   Available Since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDM098

   Plasmid

   #216818

   Purpose

   Aspergillus nidulans codon-adjusted mStayGold fluorescent protein, includes linker for C-terminal tagging.

   Depositor

   Ken Sawin

   Article

   Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

   Insert

   mStayGold
   

   Tags

   FLAG-(SGGS)x2-XTEN16-(GGGGS)x3

   Expression

   Bacterial

   Mutation

   Aspergillus nidulans codon-adjusted

   Available Since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMA-SpCas9-g10

   Plasmid

   #80793

   Purpose

   BsaI-based cloning of SpCas9 gRNA guide sequence, position 10/20/30 in the array

   Depositor

   Yonglun Luo

   Article

   Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

   Insert

   CRISPR gRNA expression cassette (for SpCas9)
   

   Use

   CRISPR

   Tags

   na

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMA-SpCas9-g9

    Plasmid

    #80792

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 9/19/29 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMA-SpCas9-g8

    Plasmid

    #80791

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 8/18/28 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEM-T Easy-AtpU6-26

    Plasmid

    #160218

    Purpose

    AtpU6-26 Golden Gate level 0 piece to express gRNAs

    Depositor

    Matias Kirst

    Article

    Triozzi et al Tree Physiol. 2021 May 7. pii: 6270869.

    Insert

    pAtU6-26 promoter
    

    Use

    Golden gate level 0 piece to express grnas

    Available Since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. BB2_EF

    Plasmid

    #98538

    Purpose

    empty BB2 for transcription unit cloning

    Depositor

    Diethard Mattanovich, Michael Sauer, Brigitte Gasser

    Article

    Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBT100-neo

    Plasmid

    #123137

    Purpose

    Golden Gate donor vector for the assembly of EGFP-based mammalian recombinase reporters.

    Depositor

    David Liu

    Article

    Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

    Insert

    Neomycin, poly-A terminator
    

    Use

    Synthetic Biology; Golden gate donor vector

    Promoter

    none

    Available Since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. HSA_3b*_AP

    Plasmid

    #238899

    Purpose

    Plasmid insert contains a gene sequence for Human serum albumin for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

    Depositor

    Joseph Brock

    Article

    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

    Insert

    Recombinant Human Serum Albumin (HSA) 3b*
    

    Use

    Synthetic Biology

    Available Since

    Sept. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. C-term_TEV_aMFx1 (4a)

    Plasmid

    #238903

    Purpose

    Plasmid insert contains the aMFx1 C-terminal tag sequence with the TEV cleavage site for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

    Depositor

    Joseph Brock

    Article

    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

    Insert

    C-term_TEV_aMFx1
    

    Use

    Synthetic Biology

    Available Since

    Sept. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. C-term_C-myc (4a)

    Plasmid

    #238907

    Purpose

    Plasmid insert contains the C-myc C-terminal tag sequence for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

    Depositor

    Joseph Brock

    Article

    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

    Insert

    C-term_C-myc
    

    Use

    Synthetic Biology

    Available Since

    Sept. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. SP_H3

    Plasmid

    #228189

    Purpose

    Plasmid contains the secretion signal peptide sequence of the yeast CIS3 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

    Depositor

    Joseph Brock

    Article

    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

    Insert

    sp_P47001_CIS3_YEAST:0-21
    

    Use

    Synthetic Biology

    Available Since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. SP_H4

    Plasmid

    #228190

    Purpose

    Plasmid contains the secretion signal peptide sequence of the human CCL8 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

    Depositor

    Joseph Brock

    Article

    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

    Insert

    sp_P80075_CCL8_HUMAN:0-23
    

    Use

    Synthetic Biology

    Available Since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. SP_H5

    Plasmid

    #228191

    Purpose

    Plasmid contains the secretion signal peptide sequence of the human SDC4 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

    Depositor

    Joseph Brock

    Article

    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

    Insert

    sp_P31431_SDC4_HUMAN:0-20
    

    Use

    Synthetic Biology

    Available Since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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