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We narrowed to 4,839 results for: pcr

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Showing: 781 - 800 of 4839 results

  1. pJMP10233_pCRISPRt-H

    Plasmid
    #225832
    Purpose
    CRISPRt helper plasmid to construct CRISPRt and CRISPRtOE strains. Cas678, TniQ, TnsABC expression.
    Depositor
    Jason Peters
    Publication
    Banta et al bioRxiv 2024.03.01.582922
    Insert
    Cas678, TniQ, TnsABC
    Use
    Synthetic Biology; Crispr associated transpositio…
    Expression
    Bacterial
    Available since
    Aug. 31, 2026
    Availability
    Academic Institutions and Nonprofits only
    225832-plasmid-map-sequence-id-448350

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  2. pCR175

    Plasmid
    #240640
    Purpose
    Template for in vitro transcription of mTurquoise2 mRNA using SP6 polymerase.
    Depositor
    John Pringle
    Insert
    mTurquoise2
    Use
    Vertebrate gene expression and general in vitro t…
    Promoter
    SP6
    Available since
    Aug. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    240640-plasmid-map-sequence-id-483016

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  3. pCR178

    Plasmid
    #240641
    Purpose
    Template for in vitro transcription of mScarlet-I mRNA using SP6 polymerase.
    Depositor
    John Pringle
    Insert
    mScarlet-I
    Use
    Vertebrate gene expression and general in vitro t…
    Promoter
    SP6
    Available since
    Aug. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    240641-plasmid-map-sequence-id-482342

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  4. pCR182

    Plasmid
    #240642
    Purpose
    Template for in vitro transcription of AmNeonGreen mRNA using SP6 polymerase.
    Depositor
    John Pringle
    Insert
    AmNeonGreen
    Use
    Vertebrate gene expression and general in vitro t…
    Mutation
    Codon-optimized for Exaiptasia diaphana, amino ac…
    Promoter
    SP6
    Available since
    Aug. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    240642-plasmid-map-sequence-id-482913

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  5. pCR197

    Plasmid
    #240643
    Purpose
    Expression of the tri-cistronic construct NES-AmNeonGreen-P2A-mTurquoise2-NLS-T2A-mScarlet-I-PTS1 in Exaiptasia diaphana; In vitro transcription of the same construct via SP6 polymerase
    Depositor
    John Pringle
    Inserts
    AIPGENE865 promoter
    SP6 promoter
    AmNeonGreen
    mTurquoise2
    mScarlet-I
    Use
    Gene expression and genomic integration in fish
    Tags
    NES (from Exaiptasia diaphana MEK2), NLS (from SV…
    Mutation
    Codon-optimized for Exaiptasia diaphana, amino ac…
    Available since
    Aug. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    240643-plasmid-map-sequence-id-482910

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  6. pCR241

    Plasmid
    #240644
    Purpose
    Expression of AmNeonGreen-13myc in Exaiptasia diaphana; In vitro transcription of the same construct via SP6 polymerase
    Depositor
    John Pringle
    Inserts
    AIPGENE865 promoter
    SP6 promoter
    AmNeonGreen
    Use
    Gene expression and genomic integration in fish
    Tags
    13myc
    Mutation
    Codon-optimized for Exaiptasia diaphana, amino ac…
    Available since
    Aug. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    240644-plasmid-map-sequence-id-482908

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  7. pCR158

    Plasmid
    #240639
    Purpose
    Expression of NLS-Kaede in Exaiptasia diaphana
    Depositor
    John Pringle
    Inserts
    AIPGENE865 promoter
    Kaede
    Use
    Expression of nls-kaede in exaiptasia diaphana
    Tags
    SV40 NLS
    Mutation
    naturally occurring 749-bp insertion at bp 628 (f…
    Promoter
    Exaiptasia diaphana Elongation Factor 1 alpha (AI…
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    240639-plasmid-map-sequence-id-478443

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  8. pRSF_PcRbcLS

    Plasmid
    #229519
    Purpose
    Expresses Phaeoceros carolinianus Rubisco large and small subunit (C terminus hexahistidine)
    Depositor
    Laura Gunn
    Publication
    Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849.
    Inserts
    Rubisco large subunit
    Rubisco small subunit
    Tags
    hexahistidine
    Expression
    Bacterial
    Mutation
    N terminus chloroplast transit peptide truncation
    Promoter
    T7
    Available since
    June 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    229519-plasmid-map-sequence-id-457204

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  11. pCRISPR3_BC2

    Plasmid
    #191857
    Purpose
    Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: ATCATG) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.
    Depositor
    Kimberly Reynolds
    Publication
    Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.
    Type
    Empty backbone
    Use
    CRISPR
    Available since
    Jan. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    191857-plasmid-map-sequence-id-381604

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Showing: 781 - 800 of 4839 results