We narrowed to 19,051 results for: Tec

1. A*24:07:01

   Plasmid

   #203283

   Purpose

   Overexpress HLA allele

   Depositor

   Derin Keskin, Catherine Wu

   Article

   Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

   Insert

   A*24:07:01 (HLA-A Human)
   

   Expression

   Mammalian

   Available Since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV_APOBEC1-UdgX-NG-nCas9

   Plasmid

   #163533

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   APOBEC1-UdgX-NG-nCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

   Available Since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV_UdgX-EE-UdgX-nCas9-UdgX

   Plasmid

   #163566

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   UdgX-EE-UdgX-nCas9-UdgX
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)EE (R126E, R132E)

   Available Since

   Jan. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-Cerulean3-Cerulean3-FLARE-Cameleon

   Plasmid

   #123353

   Purpose

   Cyan-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   Cerulean3-Cerulean3-FLARE-Cameleon
   

   Tags

   6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-mA3-BE3

   Plasmid

   #113419

   Purpose

   Expresses mA3-BE3 in mammalian cells

   Depositor

   Jia Chen

   Article

   Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

   Insert

   mA3-BE3 (Apobec3 Mouse, S. pyogenes and Bacteriophage PBS2)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV_POLD2-APOBEC1-UdgX-nCas9-UdgX

   Plasmid

   #163543

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   POLD2-APOBEC1-UdgX-nCas9-UdgX
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)

   Available Since

   Dec. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-mCherry-mCherry-FLARE-AKAR

   Plasmid

   #123333

   Purpose

   Red-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   mCherry-mCherry-FLARE-AKAR
   

   Tags

   6xHIS, T7 tag (gene 10 leader), Xpress (TM) tag, …

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. MuHKI-pGFPN3

   Plasmid

   #21919

   Depositor

   Hossein Ardehali

   Article

   Sun et al Mol Cell Biol. 2008 Feb . 28(3):1007-17.

   Insert

   Mutant huamn HKI (with a mutation in the catalytic site in the C-terminal half of the enzyme) in pGFP-N3 (HK1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   This is the full length human HKI cDNA sequence w…

   Available Since

   Nov. 2, 2009

   Availability

   Academic Institutions and Nonprofits only

9. pCMV-hA3G-BE3

   Plasmid

   #113415

   Purpose

   Expresses hA3G-BE3 in mammalian cells

   Depositor

   Jia Chen

   Article

   Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

   Insert

   hA3G-BE3 (APOBEC3G S. pyogenes and Bacteriophage PBS2, Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJS647

    Plasmid

    #183176

    Purpose

    PYR1 mutagenesis plasmid compatible with pJS646 Y2H vector

    Depositor

    Timothy Whitehead

    Article

    Beltran et al Nat Biotechnol. 2022 Jun 20. pii: 10.103

    Insert

    PYR1 (PYR1 Mustard Weed)
    

    Expression

    Bacterial

    Available Since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-dCpf1-eBE

    Plasmid

    #107688

    Purpose

    Expresses dLbCpf1-eBE in mammalian cells

    Depositor

    Jia Chen

    Article

    Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    dLbCpf1-eBE
    

    Expression

    Mammalian

    Mutation

    dLbCpf1(D832A/E925A/D1148A)

    Promoter

    CMV

    Available Since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. MSP2135

    Plasmid

    #72248

    Purpose

    Human expression plasmid for SpCas9-HF2 variant: CMV-T7-humanSpCas9-HF2(N497A, R661A, Q695A, Q926A, D1135E)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 HF2(N497A/R661A/Q695A/Q926A/D1135E)-NLS-3xFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, Q926A, and D1135E

    Promoter

    CMV

    Available Since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. non-targeting control gRNA (BRDN0001145694)

    Plasmid

    #80191

    Purpose

    3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    Non-targeting control gRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. SpCas9-NRCH-ABE8.17-m+V106W

    Plasmid

    #198551

    Purpose

    Expresses human codon-optimized SpCas9-NRCH-ABE8.17-m+V106W and blasticidin resistance: EFS promoter-SpCas9-NRCH-ABE8.17-m+V106W-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NRCH-ABE8.17-m+V106W
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA_B2AR-s-pep

    Plasmid

    #47438

    Purpose

    FRET sensor for beta2-adrenergic receptor

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Malik et al J Biol Chem. 2013 Jun 14;288(24):17167-7

    Insert

    beta2-adrenergic receptor (ADRB2 Human)
    

    Tags

    10 nm ER/K helix, HA, mCerulean, mCitrine, and s-…

    Expression

    Mammalian

    Promoter

    pCMV

    Available Since

    Sept. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

16. p168-759

    Plasmid

    #62619

    Purpose

    ePathBrick pETM6 vector encoding CsF3H, AaDFR, DuLAR with Protein Scaffold GBD:SH3:PDZ (1:1:4)

    Depositor

    Mattheos Koffas

    Article

    Zhao et al Metab Eng. 2014 Dec 17;28C:43-53. doi: 1

    Inserts

    flavaone 3B-hydroxylase
    dihydroflavonol 4-reductase
    leucoanthocyanidin reductase
    Scaffold Protein encoding GBD:SH3:PDZ (1:1:4)
    

    Use

    Synthetic Biology

    Tags

    GBD, PDZ, and SH3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-hSyn-GACh2.0

    Plasmid

    #121921

    Purpose

    express the genetically-encoded fluorescent acethycholine(ACh) sensor GACh2.0

    Depositor

    Yulong Li

    Article

    Jing et al Nat Biotechnol. 2018 Sep;36(8):726-737.

    Insert

    GPCR activation based ACh sensor GACh2.0
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. OMM-long-CFAST10

    Plasmid

    #233598

    Purpose

    Expression of CFAST10 on the OMM membrane after a long linker

    Depositor

    Riccardo Filadi

    Article

    Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

    Insert

    CFAST10
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CaMKII-iGECI-NES

    Plasmid

    #160424

    Purpose

    AAV plasmid for CaMKII-driven expression of a genetically-encoded calcium indicator iGECI with a nuclear export signal sequence

    Depositor

    Vladislav Verkhusha

    Article

    Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

    Insert

    iGECI-NES
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CaMKII

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-Venus-Venus-FLARE-Cameleon

    Plasmid

    #123351

    Purpose

    Yellow-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    Venus-Venus-FLARE-Cameleon
    

    Tags

    6xHIS, T7 tag (gene 10 leader), Venus, and Xpress…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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