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pCMV 3xFLAG-LC3C G126

Plasmid

#123096

Purpose

Expresses 3xFLAG-LC3C G126 in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3C (MAP1LC3C Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Deleted amino acids 127-147. Stop codon after G12…

Promoter

CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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CKAP2-mNeonGreen pHAT

Plasmid

#175947

Purpose

Bacterial expression of mmCKAP2-mNeonGreen, N-terminal 6xHis-tag, C-terminal Strep-tagII

Depositor

Susanne Bechstedt

Article

McAlear et al Elife. 2022 Jan 14;11. pii: 72202. doi:

Insert

CKAP2 (Ckap2 Mouse)

Tags

6His, StrepTagII, and mNeonGreen

Expression

Bacterial

Promoter

T7

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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Bin1 delta SH3

Plasmid

#168283

Purpose

Express SH3-deleted Bin1 mutant (aa 1-404) in pEGFP-N1 vector.

Depositor

Cheng-han Yu

Article

Cao et al Commun Biol. 2020 Mar 13;3(1):117. doi:

Insert

Bin1 (Bin1 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

aa 1-404

Available Since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 FOXM

Plasmid

#67118

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

FOXM (FOXM1 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
KK001: pMVP/SB/Hygro-DEST

Plasmid

#121867

Purpose

pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Hygro selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Sleeping beauty transposon, pmvp gateway destinat…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2067_pCAG-PV1-GAI-L1-CTD-ENTRY-BGHpA

Plasmid

#108845

Purpose

Insert protein fragment into KpnI/EcoRI to fuse to GAI tag on N-terminus

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Type

Empty backbone

Use

Synthetic Biology

Tags

GAI

Expression

Mammalian

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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p3XFlag-CARMIL2

Plasmid

#118740

Purpose

To express CARMIL2 with Flag tag on N-terminus

Depositor

John Cooper

Article

Lanier et al Mol Biol Cell. 2015 Dec 15;26(25):4577-8

Insert

CARMIL2 (CARMIL2 Human)

Tags

3x Flag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pBW2065_pCAG-PV1-GID1-L1-CTD-ENTRY-BGHpA

Plasmid

#108843

Purpose

Insert protein fragment into KpnI/EcoRI to fuse to GID1 tag on N-terminus

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Type

Empty backbone

Use

Synthetic Biology

Tags

GID1

Expression

Mammalian

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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attB‐mCh‐Poly(A)-PuroR

Plasmid

#65529

Purpose

PuroR containing plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

attB-mCherry

Use

Mouse Targeting; Bxb1

Expression

Mammalian

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 GTF2E1

Plasmid

#67089

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

GTF2E1 (GTF2E1 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
IX301: pMVP (L3-L2) myc epitope tag + polyA

Plasmid

#121753

Purpose

pMVP L3-L2 entry plasmid, contains myc epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

myc epitope tag + polyA

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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LN-entry

Plasmid

#128012

Purpose

Vector containing the lambdaN-entry expression cassette and a separate expression cassette driving mCherry via the traffic jam enhancer

Depositor

Julius Brennecke

Article

Batki et al Nat Struct Mol Biol. 2019 Aug;26(8):720-

Insert

LN-3xFlag

Use

Lamdan entry vector

Tags

LN-3xFlag

Available Since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 EBNA1BP2

Plasmid

#67072

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

EBNA1BP2 EBNA1BP2 EBNA1 binding protein 2 (EBNA1BP2 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
(B) HYG marker(R) (SBE264)

Plasmid

#195013

Purpose

hygromycin (HYG) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tGPD

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

pXYL+HYG+tGPD

Expression

Bacterial

Promoter

pXYL

Available Since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

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(B) BLE marker(R) (SBE262)

Plasmid

#195012

Purpose

bleomycin (BLE) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tGPD

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

pXYL+BLE+tGPD

Expression

Bacterial

Promoter

pXYL

Available Since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

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Cntn4.2-AP-His

Plasmid

#71942

Purpose

Expresses the entire Contactin 2, isoform 2 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Cntn4.2 (Cntn4 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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FB027

Plasmid

#119712

Purpose

Multipartite assembly obtained by combining FB parts FB026+FB003 into pDGB3omega1. Used for fluorescent tagging of fungi (Hyg resistance +YFP) according to FungalBraid modular DNA assembly for ATMT

Depositor

Jose Marcos

Article

Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

Insert

PgpdA::YFP::TtrpC(←)-PtrpC::hph::Ttub (→)

Use

Synthetic Biology

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-C2_3X Flag

Plasmid

#194161

Purpose

Expresses eGFP fluorescent protein with a 3X flag tag

Depositor

Stefan Stamm

Article

Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129

Insert

GFP with 3X Flag

Tags

3x Flag

Expression

Mammalian

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pWZL Blast H-Ras G12V E37G

Plasmid

#12277

Depositor

Bob Weinberg

Article

Rangarajan et al Cancer Cell. 2004 Aug . 6(2):171-83.

Insert

H-Ras G12V E37G (HRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

Contains G12V activating mutation. The E37G mutat…

Available Since

Aug. 3, 2006

Availability

Academic Institutions and Nonprofits only
pSF4 TetCMV intron 4xGCN4 Renilla FKBP STOP 24xMS2v5 SV40 CTE polyA

Plasmid

#104984

Purpose

Expresses ST-Renilla reporter in mammalian cells

Depositor

Jeffrey Chao

Article

Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.

Insert

Renilla luciferase

Tags

24xMS2v5 stem loops in 3'UTR, 4xGCN4 repeats…

Expression

Bacterial and Mammalian

Promoter

TetCMV

Available Since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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