We narrowed to 9,577 results for: bli

1. SmBiT-KRAS4B(G12D) BiBiT-Ready Vector

   Plasmid

   #236798

   Purpose

   Express SmBiT-KRAS4B(G12D) Fusion Protein in Mammalian Cells under a CMV promoter

   Depositor

   Promega Corporation

   Has Service

   DNA

   Insert

   KRAS4B(G12D) (KRAS Human)
   

   Tags

   SmBiT

   Expression

   Mammalian

   Mutation

   G12D

   Promoter

   CMV

   Available Since

   July 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pSG297-(Sp-gRNA)-(Sa-gRNA)-(PuroR_T2A_BFP(C>T screening))

   Plasmid

   #239448

   Purpose

   Orthogonal dual Cas9 gRNA and BFP reporter lentivirus cassette plasmid. For use with S. pyogenes and S. aureus gRNAs and includes a BFP reporter which reports on C-to-T editing.

   Depositor

   Alexis Komor

   Inserts

   S.p. gRNA backbone
   S.a. gRNA backbone
   PuroR-T2A-BFP(C>T_screening)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   BFP: T65S, S72S, V145F, K206K, H231L

   Promoter

   EF1alpha, U6, and mU6

   Available Since

   June 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. rTetR-GFP-p65

   Plasmid

   #232633

   Purpose

   p65 activation domain coupled to reverse Tet repressor; labeled with GFP; binds tetO sites upon doxycycline addition

   Depositor

   Karsten Rippe

   Insert

   rTetR-GFP-p65 (RELA Human, Synthetic)
   

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   March 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pdCas9-GFP-p65

   Plasmid

   #232630

   Purpose

   p65 activation domain coupled to catalytically inactive dCas9 for localization; contains GFP for labeling and as a spacer

   Depositor

   Karsten Rippe

   Insert

   dCas9-GFP-p65 (RELA Human, Synthetic)
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   March 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. Kif3A-EGFP-SNAP

   Plasmid

   #229852

   Purpose

   Kif3A (aa1-342)-Kif1A neck linker(17aa) fused to Kin1 coiled coil (aa345-406)-EGFP-SNAP-his. This Kif3A was used to link an oligo to the motor using the SNAP tag for single molecule experiments

   Depositor

   William Hancock

   Insert

   Kif3A (Kif3a Mouse)
   

   Tags

   6xHis, EGFP, and SNAP-tag

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLANT-2b

   Plasmid

   #231487

   Purpose

   Expresses His6-SUMO^NIT-FraB where "NIT" stands for "no internal translation", reflecting the mutation of the two Shine-Dalgarno-like sequences within the SUMO tag.

   Depositor

   Venkat Gopalan

   Inserts

   SUMO^NIT (SMT3 Budding Yeast)
   FraB
   

   Tags

   His6 tag and His6-SUMO^NIT

   Expression

   Bacterial

   Mutation

   Deleted amino acids 1-6 (MEPEES). and M83L, E84Q,…

   Promoter

   T7 promoter

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. E547* FL USP7

   Plasmid

   #229001

   Purpose

   E547-mutant of human USP7

   Depositor

   Irina Bezsonova

   Insert

   USP7 1-546 (USP7 Human)
   

   Tags

   6xHis cleavable with thrombin

   Expression

   Bacterial

   Mutation

   E547 mutated to stop codon

   Promoter

   T7

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAGGS-Twin-strep-TEV-DGKδ2

   Plasmid

   #223720

   Purpose

   Express human diacylglycerol kinase delta 2 protein (N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in mammalian cell

   Depositor

   Chiaki Murakami

   Insert

   DGKD (DGKD Human)
   

   Tags

   TEV cleavable site and Twin-Strep-tag

   Expression

   Mammalian

   Mutation

   deleted start codon

   Promoter

   CAG and chicken β-actin promoter

   Available Since

   Jan. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAGGS-PHOSPHO1-TEV-Twin-Strep

   Plasmid

   #222015

   Purpose

   Express human PHOSPHO1 protein (C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in mammalian cell

   Depositor

   Chiaki Murakami

   Insert

   PHOSPHO1 (PHOSPHO1, PHOSPHO1 Human)
   

   Tags

   TEV cleavable site and Twin-Strep-tag

   Expression

   Mammalian

   Mutation

   stop codon (TGA) is deleted

   Promoter

   chicken β-actin promoter

   Available Since

   Jan. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pWR58

    Plasmid

    #202795

    Purpose

    integrating bioluminescene reporter for Cas9 activity in S. stipitis

    Depositor

    J. Brian Robertson

    Article

    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

    Inserts

    P-TDH3/coCBG(first portion)-YUM1
    P-TEF1/ShBle/T-ACT1
    YUM1-coCBG(last portion)/T-ADH2
    URA3 and flanking sequence
    

    Expression

    Yeast

    Available Since

    Dec. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDB179-LCC-ICCG-I6M

    Plasmid

    #218690

    Purpose

    Encodes codon optimized LCC-ICCG I6M enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    LCC-ICCG
    

    Tags

    10xHis

    Expression

    Bacterial

    Available Since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDGB3_alpha2_BeYDV geminino 2.0 (no ATG)-eGFP (GB5178)

    Plasmid

    #225443

    Purpose

    Geminino 2.0-no ATG on CDS. BeYDV LIR1 + 2nd half intron ICON MP + eGFP w/o ATG + t35S:SIR + P35s + 1st half of intron from ICON MP + BeYDV LIR1.

    Depositor

    Diego Orzaez

    Insert

    BeYDV LIR1 + 2nd half intron ICON MP + eGFP w/o ATG + t35S:SIR + P35s + 1st half of intron from ICON MP + BeYDV LIR1
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. hU6-DR_BsmBI_DR-EFS-RfxCas13d-NLS-2A-Puro-WPRE CasRx pre-gRNA backbone

    Plasmid

    #219823

    Purpose

    EF1α-driven expression of RfxCas13d in mammalian cells. For cloning of pre-guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. (Adapted from plasmid #138147)

    Depositor

    Philipp Maass

    Insert

    RfxCas13d
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a core promoter

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDB179-PES-H1-L92F-Q94Y

    Plasmid

    #218705

    Purpose

    Encodes codon optimized PES-H1 L92F-Q94Y enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    PES-H1
    

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    L92F-Q94Y

    Available Since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pWR23

    Plasmid

    #202770

    Purpose

    episomal bioluminescent reporter plasmid for S. stipitis

    Depositor

    J. Brian Robertson

    Article

    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

    Inserts

    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coKanMX
    ACT1 terminator (S. stipitis)
    TDH3 promoter (S. stipitis)
    coCBG
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    

    Expression

    Yeast

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pWR10

    Plasmid

    #202710

    Purpose

    episomal bioluminescent reporter plasmid for S. stipitis

    Depositor

    J. Brian Robertson

    Article

    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

    Inserts

    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coShBle
    ACT1 terminator (S. stipitis)
    TDH3 promoter (S. stipitis)
    coCBG
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    

    Expression

    Yeast

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pWR9

    Plasmid

    #202645

    Purpose

    episomal bioluminescent reporter plasmid for S. stipitis

    Depositor

    J. Brian Robertson

    Article

    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

    Inserts

    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coHPH
    ACT1 terminator (S. stipitis)
    TDH3 promoter (S. stipitis)
    coCBG
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    

    Expression

    Yeast

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDB179-LCC-ICCG-DAQI

    Plasmid

    #218689

    Purpose

    Encodes codon optimized LCC-ICCG DAQI enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    LCC-ICCG-DAQI
    

    Tags

    10xHis

    Expression

    Bacterial

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDB179-TfCut2-L32E-S113E-T237Q

    Plasmid

    #218696

    Purpose

    Encodes codon optimized TfCut2 L32E-S113E-T237Q enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    TfCut2
    

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    L32E-S113E-T237Q

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDB179-LCC-ICCG-RIP

    Plasmid

    #218688

    Purpose

    Encodes codon optimized LCC-ICCG RIP enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    LCC-ICCG-RIP
    

    Tags

    10xHis

    Expression

    Bacterial

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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