We narrowed to 14,429 results for: nts

1. HiBiT-FZD10

   Plasmid

   #195848

   Purpose

   Expresses human FZD10 with an N-terminal HiBiT

   Depositor

   Gunnar Schulte

   Article

   Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

   Insert

   FZD10 (FZD10 Human)
   

   Use

   Tags

   HiBiT

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET20b_Pa-PiuA

   Plasmid

   #128955

   Purpose

   expresses Pseudomonas aeruginosa PiuA in E. coli

   Depositor

   James Naismith

   Article

   Moynie et al Antimicrob Agents Chemother. 2017 Mar 24

   Insert

   iron transport outer membrane receptor (PiuA) (PA4514 Pseudomonas aeruginosa PAO1)
   

   Use

   Tags

   6xHis and pelB leader

   Expression

   Bacterial

   Mutation

   Signal peptide sequence has been removed

   Promoter

   T7

   Available since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-MTS-WT-c-Src-FLAG

   Plasmid

   #44652

   Depositor

   Yoshimi Homma

   Article

   Ogura et al Biochem J. 2012 Oct 15;447(2):281-9.

   Insert

   v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)
   

   Use

   Tags

   FLAG tag and mitochondria-targeting sequence (res…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   April 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCC_01 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9-NLS-2A-Puro-WPRE

   Plasmid

   #139086

   Purpose

   Expresses human codon-optimized SpCas9 nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.

   Depositor

   Neville Sanjana

   Article

   Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

   Insert

   SpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCEP4-myc-HLA-B57

   Plasmid

   #135516

   Purpose

   Mammalian expression of myc-tagged HLA-B*57:01

   Depositor

   Erik Procko

   Article

   McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

   Insert

   HLA-B*57:1 (HLA-B Human)
   

   Use

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-TRE-flex-Clover

   Plasmid

   #135177

   Purpose

   AAV expression of TRE(TRE3G)-driven, Cre-dependent, Clover expression for fluorescent labling

   Depositor

   Tetsuo Yamamori

   Article

   Ebina et al Proc Natl Acad Sci U S A. 2019 Nov 5;116

   Inserts

   Clover
   TRE3G
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   a variant of GFP

   Promoter

   TRE3G

   Available since

   Jan. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA5-FRT/TO-N-mEGFP-PARP11

   Plasmid

   #177998

   Purpose

   Expresses N-terminally mEGFP-tagged PARP11

   Depositor

   Karla Feijs

   Article

   Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

   Insert

   PARP11 (PARP11 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. PB713B-pCMV-MITD-NeoMini-T2A-tCD19-WPRE

   Plasmid

   #174610

   Purpose

   PiggyBack transposon expressing a fusion of a tandem minigene antigens in KRAS, BRAF and CMV fused to an endosomal targeting sequence and translationally linked to human truncated CD19

   Depositor

   Stanley Riddell

   Article

   Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

   Insert

   human CD19, antigen minigene
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. px330-CD4

   Plasmid

   #136938

   Purpose

   NHEJ assay. sgRNA/Cas9 plasmid. Target DSB at human CD4; induce CD4+ deletion rearrangement by pairing w/ px330-GAPDH

   Depositor

   Jeremy Stark

   Article

   Bhargava et al J Biol Chem. 2019 Nov 21. pii: RA119.010

   Insert

   sgRNA targeting CD4 (CD4 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCS2-mkgDUX4

    Plasmid

    #21156

    Depositor

    Stephen Tapscott

    Article

    Snider et al Hum Mol Genet. 2009 Jul 1;18(13):2414-30

    Insert

    DUX4 (DUX4 Human)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. LentiU6-hASCL1 gRNA_1-MS2-Puro

    Plasmid

    #192670

    Purpose

    Lentiviral expression of sgRNA targeting hASCL1 promoter to activate human ASCL1 transcription

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Human ASCL1 activating gRNA #1 (ASCL1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAG27

    Plasmid

    #226742

    Purpose

    Expresses a reporter for a mitochondrial matrix protein

    Depositor

    Jonathan Weissman

    Article

    Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

    Insert

    COX4 (COX4I1 Human)
    

    Use

    Lentiviral

    Tags

    GFP1-10

    Expression

    Mutation

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCEP4-myc-HLA-B15

    Plasmid

    #135513

    Purpose

    Mammalian expression of myc-tagged HLA-B*15:01

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-B*15:01 (HLA-B Human)
    

    Use

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLB038 - PHS1∆P

    Plasmid

    #136285

    Purpose

    entry vector for GreenGate cloning method, C module, open reading frame of phosphatase-inactive PROPYZAMIDE-HYPERSENSITIVE 1 (PHS1) (PHS1DP)

    Depositor

    Alexis Maizel

    Article

    Vilches Barro et al Curr Biol. 2019 Aug 5;29(15):2443-2454.e

    Insert

    PHS1∆P
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYPQ266E

    Plasmid

    #161521

    Purpose

    Gateway entry clone (attL1 & attR5) for CRISPR-zSpRYCas9 PmCDA1 mediated C-T base editing

    Depositor

    Yiping Qi

    Article

    Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41

    Insert

    zSpRYCas9(D10A)-PmCDA1-UGI
    

    Use

    CRISPR; Gateway compatible zsprycas9(d10a)-pmcda1…

    Tags

    3X FLAG, NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYPQ262B-ABE8e

    Plasmid

    #161524

    Purpose

    Gateway entry clone (attL1 & attR5) for CRISPR-zSpRYCas9 ABE8e mediated A-G base editing

    Depositor

    Yiping Qi

    Article

    Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41

    Insert

    TadA8e-zSpRYCas9(D10A)
    

    Use

    CRISPR; Gateway compatible tada8e-zsprycas9(d10a)…

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYPQ265E6

    Plasmid

    #213469

    Purpose

    Gateway entry clone (attL1 & attR5) TadA-CDa-32aa linker-zSpCas9-D10A-2xUGI for C-T base editing

    Depositor

    Yiping Qi

    Article

    Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:

    Insert

    TadA-CDa-32aa linker-zSpCas9-D10A-2xUGI
    

    Use

    CRISPR; Gateway compatible tada-cda-32aa linker-z…

    Tags

    No

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3_GLP1R-V2R-NTEV-TCS-GV-2xHA

    Plasmid

    #194380

    Purpose

    Split TEV assays

    Depositor

    Michael Wehr

    Article

    Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

    Insert

    GLP1R-V2R-NTEV-TCS-GV-2xHA (GLP1R Human, Synthetic)
    

    Use

    Tags

    2xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMOD_C2515

    Plasmid

    #91083

    Purpose

    Module C, Promoter: AtU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    AtU6

    Available since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEBB HA CCDC22

    Plasmid

    #74903

    Purpose

    Express CCDC22 in mammalian cells

    Depositor

    Ezra Burstein

    Article

    Starokadomskyy et al J Clin Invest. 2013 May;123(5):2244-56.

    Insert

    CCDC22 (CCDC22 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1alpha promoter

    Available since

    June 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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