We narrowed to 9,594 results for: bli

1. pdCas9-GFP-p65

   Plasmid

   #232630

   Purpose

   p65 activation domain coupled to catalytically inactive dCas9 for localization; contains GFP for labeling and as a spacer

   Depositor

   Karsten Rippe

   Insert

   dCas9-GFP-p65 (RELA Synthetic, Human)
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   March 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. Kif3A-EGFP-SNAP

   Plasmid

   #229852

   Purpose

   Kif3A (aa1-342)-Kif1A neck linker(17aa) fused to Kin1 coiled coil (aa345-406)-EGFP-SNAP-his. This Kif3A was used to link an oligo to the motor using the SNAP tag for single molecule experiments

   Depositor

   William Hancock

   Insert

   Kif3A (Kif3a Mouse)
   

   Tags

   6xHis, EGFP, and SNAP-tag

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLANT-2b

   Plasmid

   #231487

   Purpose

   Expresses His6-SUMO^NIT-FraB where "NIT" stands for "no internal translation", reflecting the mutation of the two Shine-Dalgarno-like sequences within the SUMO tag.

   Depositor

   Venkat Gopalan

   Inserts

   SUMO^NIT (SMT3 Budding Yeast)
   FraB
   

   Tags

   His6 tag and His6-SUMO^NIT

   Expression

   Bacterial

   Mutation

   Deleted amino acids 1-6 (MEPEES). and M83L, E84Q,…

   Promoter

   T7 promoter

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. E547* FL USP7

   Plasmid

   #229001

   Purpose

   E547-mutant of human USP7

   Depositor

   Irina Bezsonova

   Insert

   USP7 1-546 (USP7 Human)
   

   Tags

   6xHis cleavable with thrombin

   Expression

   Bacterial

   Mutation

   E547 mutated to stop codon

   Promoter

   T7

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAGGS-Twin-strep-TEV-DGKδ2

   Plasmid

   #223720

   Purpose

   Express human diacylglycerol kinase delta 2 protein (N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in mammalian cell

   Depositor

   Chiaki Murakami

   Insert

   DGKD (DGKD Human)
   

   Tags

   TEV cleavable site and Twin-Strep-tag

   Expression

   Mammalian

   Mutation

   deleted start codon

   Promoter

   CAG and chicken β-actin promoter

   Available Since

   Jan. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAGGS-PHOSPHO1-TEV-Twin-Strep

   Plasmid

   #222015

   Purpose

   Express human PHOSPHO1 protein (C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in mammalian cell

   Depositor

   Chiaki Murakami

   Insert

   PHOSPHO1 (PHOSPHO1, PHOSPHO1 Human)
   

   Tags

   TEV cleavable site and Twin-Strep-tag

   Expression

   Mammalian

   Mutation

   stop codon (TGA) is deleted

   Promoter

   chicken β-actin promoter

   Available Since

   Jan. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pWR58

   Plasmid

   #202795

   Purpose

   integrating bioluminescene reporter for Cas9 activity in S. stipitis

   Depositor

   J. Brian Robertson

   Article

   Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

   Inserts

   P-TDH3/coCBG(first portion)-YUM1
   P-TEF1/ShBle/T-ACT1
   YUM1-coCBG(last portion)/T-ADH2
   URA3 and flanking sequence
   

   Expression

   Yeast

   Available Since

   Dec. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDB179-LCC-ICCG-I6M

   Plasmid

   #218690

   Purpose

   Encodes codon optimized LCC-ICCG I6M enzyme for bacterial expression

   Depositor

   Gregg Beckham

   Article

   Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

   Insert

   LCC-ICCG
   

   Tags

   10xHis

   Expression

   Bacterial

   Available Since

   Nov. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDGB3_alpha2_BeYDV geminino 2.0 (no ATG)-eGFP (GB5178)

   Plasmid

   #225443

   Purpose

   Geminino 2.0-no ATG on CDS. BeYDV LIR1 + 2nd half intron ICON MP + eGFP w/o ATG + t35S:SIR + P35s + 1st half of intron from ICON MP + BeYDV LIR1.

   Depositor

   Diego Orzaez

   Insert

   BeYDV LIR1 + 2nd half intron ICON MP + eGFP w/o ATG + t35S:SIR + P35s + 1st half of intron from ICON MP + BeYDV LIR1
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. hU6-DR_BsmBI_DR-EFS-RfxCas13d-NLS-2A-Puro-WPRE CasRx pre-gRNA backbone

    Plasmid

    #219823

    Purpose

    EF1α-driven expression of RfxCas13d in mammalian cells. For cloning of pre-guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. (Adapted from plasmid #138147)

    Depositor

    Philipp Maass

    Insert

    RfxCas13d
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a core promoter

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDB179-PES-H1-L92F-Q94Y

    Plasmid

    #218705

    Purpose

    Encodes codon optimized PES-H1 L92F-Q94Y enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    PES-H1
    

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    L92F-Q94Y

    Available Since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pWR23

    Plasmid

    #202770

    Purpose

    episomal bioluminescent reporter plasmid for S. stipitis

    Depositor

    J. Brian Robertson

    Article

    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

    Inserts

    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coKanMX
    ACT1 terminator (S. stipitis)
    TDH3 promoter (S. stipitis)
    coCBG
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    

    Expression

    Yeast

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pWR10

    Plasmid

    #202710

    Purpose

    episomal bioluminescent reporter plasmid for S. stipitis

    Depositor

    J. Brian Robertson

    Article

    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

    Inserts

    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coShBle
    ACT1 terminator (S. stipitis)
    TDH3 promoter (S. stipitis)
    coCBG
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    

    Expression

    Yeast

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pWR9

    Plasmid

    #202645

    Purpose

    episomal bioluminescent reporter plasmid for S. stipitis

    Depositor

    J. Brian Robertson

    Article

    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

    Inserts

    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coHPH
    ACT1 terminator (S. stipitis)
    TDH3 promoter (S. stipitis)
    coCBG
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    

    Expression

    Yeast

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDB179-LCC-ICCG-DAQI

    Plasmid

    #218689

    Purpose

    Encodes codon optimized LCC-ICCG DAQI enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    LCC-ICCG-DAQI
    

    Tags

    10xHis

    Expression

    Bacterial

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDB179-TfCut2-L32E-S113E-T237Q

    Plasmid

    #218696

    Purpose

    Encodes codon optimized TfCut2 L32E-S113E-T237Q enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    TfCut2
    

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    L32E-S113E-T237Q

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDB179-LCC-ICCG-RIP

    Plasmid

    #218688

    Purpose

    Encodes codon optimized LCC-ICCG RIP enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    LCC-ICCG-RIP
    

    Tags

    10xHis

    Expression

    Bacterial

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. SUMO CRYGS 16-177 Q16C

    Plasmid

    #217673

    Purpose

    N-terminally truncated human gamma S crystallin residues 16-177 with Q16C mutation for native chemical ligation

    Depositor

    Larry David

    Insert

    CRYGS (CRYGS Human)
    

    Tags

    6XHis and SUMO

    Expression

    Bacterial

    Mutation

    Glutamine 16 changed to Cysteine, and amino acids…

    Promoter

    T7

    Available Since

    May 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMamCol1

    Plasmid

    #215184

    Purpose

    Allows for stable expression of recombinant (tagged/untagged) proteins in AAVS1 locus of Human cells and transient Protein Expression in E. coli

    Depositor

    Krishna Kishore Inampudi

    Insert

    tsPurple
    

    Use

    AAV, CRISPR, and Synthetic Biology ; Recombinant …

    Tags

    SNAC-StrepII tag

    Expression

    Bacterial and Mammalian

    Promoter

    TetON 3G Bidirectional for Mammalian and Trc with…

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV/CMV-SCLM

    Plasmid

    #216758

    Purpose

    AAV2 transfer vector with the CMV promoter for ubiquitous expression of SuperClomeleon

    Depositor

    George Augustine

    Insert

    SuperClomeleon followed by WPRE and human growth hormone polyA terminator
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    S30R and 11 AA deletion in the Cerulean CFP moiet…

    Promoter

    CMV (cytomegalovirus)

    Available Since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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