We narrowed to 8,835 results for: sgRNA

1. pLentiCRISPR V2 HMBS_sg1

   Plasmid

   #244859

   Purpose

   Knockout of human HMBS

   Depositor

   Alexis Jourdain

   Article

   Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

   Insert

   HMBS sgRNA (HMBS Human)
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   Oct. 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLentiCRISPR IMMP2L sg2

   Plasmid

   #244852

   Purpose

   Knockout of human IMMP2L

   Depositor

   Alexis Jourdain

   Article

   Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

   Insert

   IMMP2L sgRNA (IMMP2L Human)
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   Oct. 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLentiCRISPR IMMP1L sg2

   Plasmid

   #244850

   Purpose

   Knockout of human IMMP1L

   Depositor

   Alexis Jourdain

   Article

   Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

   Insert

   IMMP1L sgRNA (IMMP1L Human)
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   Oct. 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLentiCRISPR IMMP1L sg1

   Plasmid

   #244849

   Purpose

   Knockout of human IMMP1L

   Depositor

   Alexis Jourdain

   Article

   Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

   Insert

   IMMP1L sgRNA (IMMP1L Human)
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   Oct. 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLentiCRISPR IMMP2L sg1

   Plasmid

   #244851

   Purpose

   Knockout of human IMMP2L

   Depositor

   Alexis Jourdain

   Article

   Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

   Insert

   IMMP2L sgRNA (IMMP2L Human)
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   Oct. 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKMW123_SpRY-Cas9_U6-entry

   Plasmid

   #244820

   Purpose

   Mammalian expression of SpRY Cas9 with Golden Gate-compatible sgRNA spacer cassette

   Depositor

   Jennifer Doudna

   Article

   Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

   Insert

   SpRY Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG-SV40 NLS and Nucleoplasmin NLS

   Expression

   Mammalian

   Mutation

   A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1…

   Promoter

   CAG, U6

   Available Since

   Sept. 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLKO-U6-sgRXRa4-1; EF1a-mScarlet

   Plasmid

   #242889

   Purpose

   For lentiviral Crispr/Cas9 mediated knockout of mouse RXRa, using a guide RNA against exon 4, coupled to EF1a-driven mScarlet

   Depositor

   Elaine Fuchs

   Article

   Stewart et al Nature. 2024 Sep;633(8029):407-416. doi:

   Insert

   sgRNA targeting Rxra
   

   Use

   CRISPR and Lentiviral

   Tags

   mScarlet

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSpCas9 (BB)-2A-GFP-RB1sg

   Plasmid

   #229857

   Purpose

   single guide RNA targeting human RB1 gene

   Depositor

   Maria Alvarado-Kristensson

   Article

   Lindstrom et al FASEB J. 2025 Mar 15;39(5):e70431. doi:

   Insert

   RB1sgRNA (RB1 Human)
   

   Tags

   The gamma-tubulin sgRNA is coexpressed with 3xFLA…

   Expression

   Mammalian

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-U6-sgLacZ-U6-sgGFP-hSyn1-mCherry

   Plasmid

   #208834

   Purpose

   Control plasmid expresses mCherry under the hSyn1 promoter with sgRNAs against LacZ and GFP

   Depositor

   Ben Emery

   Article

   Duncan et al Nat Commun. 2024 Oct 23;15(1):9148. doi:

   Insert

   mCherry
   

   Use

   AAV and CRISPR

   Expression

   Bacterial and Mammalian

   Promoter

   hSyn1

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV-U6-sgMap3K12(1)-U6-sgMap3K12(2)-hSyn1-mCherry

    Plasmid

    #208835

    Purpose

    Knockout of Map3K12 (DLK) using tandem sgRNAs, expresses mCherry under the hSyn1 promoter

    Depositor

    Ben Emery

    Article

    Duncan et al Nat Commun. 2024 Oct 23;15(1):9148. doi:

    Insert

    mCherry
    

    Use

    AAV and CRISPR

    Expression

    Bacterial and Mammalian

    Promoter

    hSyn1

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-U6-sgMap3K13(1)-U6-sgMap3K13(2)-hSyn1-mCherry

    Plasmid

    #208836

    Purpose

    Knockout of Map3K13 (LZK) using tandem sgRNAs, expresses mCherry under the hSyn1 promoter

    Depositor

    Ben Emery

    Article

    Duncan et al Nat Commun. 2024 Oct 23;15(1):9148. doi:

    Insert

    mCherry
    

    Use

    AAV and CRISPR

    Expression

    Bacterial and Mammalian

    Promoter

    hSyn1

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAVS1-TRE3G-SOX2-3xHA-P2A-tagBFP

    Plasmid

    #163701

    Purpose

    Dox-inducible SOX2-3xHA HDR knock-in cassette into the AAVS1 locus with a tagBFP fluorescent marker linked by a self-cleaving P2A peptide.

    Depositor

    Jennifer Mitchell

    Article

    Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

    Inserts

    SOX2-3xHA-P2A-tagBFP (SOX2 Human)
    rtTA
    T2-sgRNA
    

    Use

    AAV and CRISPR

    Tags

    3x-HA and P2A-tagBFP

    Expression

    Mammalian

    Promoter

    CAG and TRE3G

    Available Since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMXS-IRES-BLAST flag-CAD R2187A

    Plasmid

    #169827

    Purpose

    Expresses C-terminal flag-tagged CAD with mutation at reported trimerization interface of the ATCase domain of CAD

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    R2187A, T456A S1406A S1859A; TCCC -> AGTC sile…

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMXS-IRES-BLAST flag-CAD M1601E

    Plasmid

    #169828

    Purpose

    Expresses C-terminal flag-tagged CAD with mutation at reported dimerization interface of the DHOase domain

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    M1601E; TCCC -> AGTC silent mutations at nt527…

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMXS-IRES-BLAST flag-CAD H1734A H1741A

    Plasmid

    #169830

    Purpose

    Expresses C-terminal flag-tagged CAD with mutations at reported dimerization interface of the DHOase domain

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    H1734A H1741A; TCCC -> AGTC silent mutations a…

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMXS-IRES-BLAST flag-CAD delta Loop1

    Plasmid

    #169831

    Purpose

    Expresses C-terminal flag-tagged CAD with deletion of amino acids F1366 - P1376 in allosteric domain

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Deletion of amino acids F1366 - P1376; TCCC ->…

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMXS-IRES-BLAST flag-CAD delta Loop2

    Plasmid

    #169832

    Purpose

    Expresses C-terminal flag-tagged CAD with deletion of amino acids R1398 - S1407 in allosteric domain

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Deletion of amino acids R1398 - S1407; TCCC ->…

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMXS-IRES-BLAST flag-CAD S1406A

    Plasmid

    #169820

    Purpose

    Expresses C-terminal flag-tagged CAD with mutation of reported PKA phosphorylation site

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    S1406A; TCCC -> AGTC silent mutations at nt527…

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMXS-IRES-BLAST flag-CAD S1859E

    Plasmid

    #169822

    Purpose

    Expresses C-terminal flag-tagged CAD with mutation of reported S6K phosphorylation site

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    S1859E; TCCC -> AGTC silent mutations at nt527…

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMXS-IRES-BLAST flag-CAD T456A S1406A

    Plasmid

    #169823

    Purpose

    Expresses C-terminal flag-tagged CAD with mutation of reported Erk1/2 and PKA phosphorylation site

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    T456A S1406A; TCCC -> AGTC silent mutations at…

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge