We narrowed to 9,135 results for: sgRNA

Showing: 8041 - 8060 of 9135 results

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pCas45

Plasmid

#82390

Purpose

sgRNA targeting YFP with additional bases 5' end like pCas34, but from pJ23119 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Promoter

PJ23119

Available Since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCas57

Plasmid

#82395

Purpose

sgRNA targeting YFP +111 from TSS; template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Promoter

PEZ3

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCas56

Plasmid

#82394

Purpose

sgRNA targeting YFP +46 from TSS; template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Promoter

PEZ3

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCas55

Plasmid

#82393

Purpose

sgRNA targeting YFP +172 from TSS; non-template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Promoter

PEZ3

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCas52

Plasmid

#82392

Purpose

sgRNA targeting YFP +52 from TSS; non-template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Promoter

PEZ3

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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CMV-sgE1TSS-short-3'box

Plasmid

#92165

Purpose

sgRNA expression vector for Evx1as RNA tethering assay

Depositor

Xiaohua Shen

Article

Luo et al Cell Stem Cell. 2016 May 5;18(5):637-52.

Insert

Evx1as short isoform

Use

CRISPR

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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dual-sgRNA CRISPRi library for PARP inhibitor response

Pooled Library

#238134

Purpose

This library can be used in genetic interaction experiments for mapping genetic interaction network in DNA repair and PARP inhibitor response.

Depositor

Brittany Adamson

Article

Simpson et al bioRxiv [Preprint]. 2023 Aug 20:2023.08.

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available Since

Jan. 27, 2026

Availability

Academic Institutions and Nonprofits only
Pooled sgRNA Libraries Set 1 for Pseudomonas aeruginosa PA14

Pooled Library

#234855

Purpose

A CRISPRi library for inducible, genome-wide gene repression in Pseudomonas aeruginosa PA14.

Depositor

Xue Liu

Article

Zhang et al Nat Commun. 2025 Jul 1;16(1):5870. doi:

Expression

Bacterial

Available Since

June 5, 2025

Availability

Academic Institutions and Nonprofits only
pENTR1A_mmFbxw7(res) (closed)

Plasmid

#160105

Purpose

Expresses murine Fbxw7 alpha with a T199 silent mutation in mammalian cells. The T199 silent mutation confers resistance to the 5'-TGAACATGGTACAAGGCCAG-3' sgRNA

Depositor

Rizwan Haq

Article

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Insert

Fbxw7alpha (Fbxw7 Mouse)

Use

Gateway Cloning

Expression

Mammalian

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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Mouse CRISPRi sgRNA library Dolomiti in backbone XPR_050, Set B

Pooled Library

#104091

Purpose

Crispri

Depositor

John Doench, David Root

Article

Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

Use

Lentiviral

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only
pAAV-CMV-FLEX-SaCas9-U6-sgTh

Plasmid

#159901

Purpose

Mutagenesis of Th

Depositor

Larry Zweifel

Insert

Th (Th Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgPenk

Plasmid

#159916

Purpose

Mutagenesis of Penk

Depositor

Larry Zweifel

Insert

Penk (Penk Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pXPR_023d sgCiPELO #1

Plasmid

#228932

Purpose

Expression of dCas9-KRAB and sgRNA targeting PELO

Depositor

Francisca Vazquez

Article

Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

Insert

PELO gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pXPR_023d sgCiPELO #8

Plasmid

#228934

Purpose

Expression of dCas9-KRAB and sgRNA targeting PELO

Depositor

Francisca Vazquez

Article

Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

Insert

PELO gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD051d_sgCiPELO #2

Plasmid

#229018

Purpose

Expression of CRISPRi PELO sgRNA #2

Depositor

Francisca Vazquez

Article

Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

Insert

PELO (PELO Human)

Use

CRISPR

Expression

Mammalian

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.minBG-EGFP-W3SL_BbsI(GGA)

Plasmid

#231362

Purpose

minBG-driven EGFP, containing cassette for BbsI-based Golden Gate assembly (e.g. of sgRNA cassette(s)). Can be used as 'no guide' control.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

EGFP

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.Ple155-CI-EGFP-W3SL_BbsI(GGA)

Plasmid

#231363

Purpose

Ple155-driven EGFP, containing cassette for BbsI-based Golden Gate assembly (e.g. of sgRNA cassette(s)). Can be used as 'no guide' control.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

EGFP

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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SAM-DNMT3A empty

Plasmid

#213164

Purpose

Empty vector (no sgRNA) for induction of global DNA methylation.

Depositor

Sefi Rosenbluh

Article

Hosseinpour et al Nat Commun. 2024 Dec 1;15(1):10449. doi:

Type

Empty backbone

Use

CRISPR, Lentiviral, and Synthetic Biology

Expression

Mammalian

Promoter

E1Fa and U6

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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SAM-DNMT3A-inactiveEmpty

Plasmid

#213168

Purpose

Empty Vector (no sgRNA) used as a negative control (inactive DNMT3A) for induction of global DNA methylation.

Depositor

Sefi Rosenbluh

Article

Hosseinpour et al Nat Commun. 2024 Dec 1;15(1):10449. doi:

Type

Empty backbone

Use

CRISPR, Lentiviral, and Synthetic Biology

Expression

Mammalian

Promoter

E1Fa and U6

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS1831 All-in-one AAV-U1a-NmeABE-8e-2xBPSV40-miniU6-Fah

Plasmid

#199264

Purpose

Single AAV vector for expressing N-terminal fusion Nme2Cas9-ABE8e and one miniU6 driven sgRNA targeting the point mutation in the mouse Fah gene of a HT1 mouse model

Depositor

Erik Sontheimer

Article

Zhang et al GEN Biotechnol. 2022 Jun 1;1(3):285-299.

Insert

NmeABE8e

Use

AAV and CRISPR

Tags

NLS

Expression

Mammalian

Available Since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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