We narrowed to 15,991 results for: LEA

1. pAAV-TRE-FRT-2xtTA

   Plasmid

   #191205

   Purpose

   Flpo dependent tTA expression under the control of TRE promotor

   Depositor

   Janelia Viral Tools

   Article

   Glaser et al Nat Methods. 2022 May;19(5):613-619. doi

   Insert

   2xtTA
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. ATX3 13Q- I77K Q78K W87K

   Plasmid

   #185908

   Purpose

   Expresses ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track-I77K, Q78K,W87K (N-terminal ) fused with His-Tag and TEV cleavage sequence (C-terminal).

   Depositor

   Sandra Macedo Ribeiro

   Article

   Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111

   Insert

   ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track and point mutations I77K, Q78K and W87K (ATXN3 Synthetic, Human)
   

   Tags

   6x His-Tag and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Point mutations in Atx3 gene I77K Q78K and W87K

   Available Since

   Aug. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-T7-NLS(SV40)-anCas(FCA)-6xHis-NLS(SV40)

   Plasmid

   #185701

   Purpose

   CMV and T7 promoter expression plasmid for human codon usage anCas(FCA) with an N-terminal NLS and C-terminal NLS

   Depositor

   Raul Perez-Jimenez

   Article

   Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

   Insert

   anCas(FCA)
   

   Tags

   6xHis-NLS and NLS

   Expression

   Mammalian

   Mutation

   optimized for human codon usage

   Promoter

   CMV and T7

   Available Since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-T7-NLS(SV40)-anCas(PDCA)-6xHis-NLS(SV40)

   Plasmid

   #185705

   Purpose

   CMV and T7 promoter expression plasmid for human codon usage anCas(PDCA) with an N-terminal NLS and C-terminal NLS

   Depositor

   Raul Perez-Jimenez

   Article

   Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

   Insert

   anCas(PDCA)
   

   Tags

   6xHis-NLS and NLS

   Expression

   Mammalian

   Mutation

   optimized for human codon usage

   Promoter

   CMV and T7

   Available Since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-T7-NLS(SV40)-anCas(SCA)-6xHis-NLS(SV40)

   Plasmid

   #185703

   Purpose

   CMV and T7 promoter expression plasmid for human codon usage anCas(SCA) with an N-terminal NLS and C-terminal NLS

   Depositor

   Raul Perez-Jimenez

   Article

   Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

   Insert

   anCas(SCA)
   

   Tags

   6xHis-NLS and NLS

   Expression

   Mammalian

   Mutation

   optimized for human codon usage

   Promoter

   CMV and T7

   Available Since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-T7-NLS(SV40)-anCas(PCA)-6xHis-NLS(SV40)

   Plasmid

   #185704

   Purpose

   CMV and T7 promoter expression plasmid for human codon usage anCas(PCA) with an N-terminal NLS and C-terminal NLS

   Depositor

   Raul Perez-Jimenez

   Article

   Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

   Insert

   anCas(PCA)
   

   Tags

   6xHis-NLS and NLS

   Expression

   Mammalian

   Mutation

   optimized for human codon usage

   Promoter

   CMV and T7

   Available Since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV-T7-NLS(SV40)-anCas(BCA)-6xHis-NLS(SV40)

   Plasmid

   #185702

   Purpose

   CMV and T7 promoter expression plasmid for human codon usage anCas(BCA) with an N-terminal NLS and C-terminal NLS

   Depositor

   Raul Perez-Jimenez

   Article

   Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

   Insert

   anCas(BCA)
   

   Tags

   6xHis-NLS and NLS

   Expression

   Mammalian

   Mutation

   optimized for human codon usage

   Promoter

   CMV and T7

   Available Since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pENTR4 CDS A. thaliana IMPORTIN ALPHA 3 / MOS6

   Plasmid

   #175820

   Purpose

   pENTR plasmid with CDS of Arabidopsis thaliana IMPORTIN ALPHA ISOFORM 3 / MOS6, lacks start ATG and includes stop codon for N-terminal epitope tags

   Depositor

   Lennart Wirthmueller

   Article

   Ludke et al Plant J. 2021 Feb;105(4):994-1009. doi:

   Insert

   IMPORTIN ALPHA ISOFORM 3 (MOS6 Mustard Weed)
   

   Use

   Pentr plasmid for gateway cloning

   Tags

   none

   Promoter

   none

   Available Since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBIG1b_nsp10-6His-3xFlag (SARS-CoV-2)

   Plasmid

   #169162

   Purpose

   Baculoviral transfer vector to express SARS-CoV-2 nsp10 in insect cells

   Depositor

   John Diffley

   Article

   Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

   Insert

   nsp10-6His-3xFlag (ORF1ab SARS-CoV-2, Synthetic)
   

   Tags

   6His-3xFlag

   Expression

   Insect

   Mutation

   Codon optimised for insect cells (Spodoptera frug…

   Promoter

   Polyhedrin

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBIG1a_3xFlag-6His-nsp14 (SARS-CoV-2)

    Plasmid

    #169163

    Purpose

    Baculoviral transfer vector to express SARS-CoV-2 nsp14 in insect cells

    Depositor

    John Diffley

    Article

    Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

    Insert

    3xFlag-6His-nsp14 (ORF1ab SARS-CoV-2, Synthetic)
    

    Tags

    3xFlag-6His

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pINTO-N3::hTET3 V908L

    Plasmid

    #136370

    Purpose

    Expresses epitope-tagged human TET3 V908L variant in mammalian cells

    Depositor

    Roberto Bonasio

    Article

    Beck et al Am J Hum Genet. 2020 Jan 3. pii: S0002-9

    Insert

    TET3 (TET3 Human)
    

    Tags

    2xStrep-tagII, FLAG, and HA

    Expression

    Mammalian

    Mutation

    V908L

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pINTO-N3::hTET3 V1089M

    Plasmid

    #136369

    Purpose

    Expresses epitope-tagged human TET3 V1089M variant in mammalian cells

    Depositor

    Roberto Bonasio

    Article

    Beck et al Am J Hum Genet. 2020 Jan 3. pii: S0002-9

    Insert

    TET3 (TET3 Human)
    

    Tags

    2x Strep-tagII, FLAG, and HA

    Expression

    Mammalian

    Mutation

    V1089M

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pINTO-N3::hTET3 A1076T

    Plasmid

    #136368

    Purpose

    Expresses epitope-tagged human TET3 A1076C variant in mammalian cells

    Depositor

    Roberto Bonasio

    Article

    Beck et al Am J Hum Genet. 2020 Jan 3. pii: S0002-9

    Insert

    TET3 (TET3 Human)
    

    Tags

    2xStrepTagII, FLAG, and HA

    Expression

    Mammalian

    Mutation

    A1076T

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pINTO-N3::hTET3 R752C

    Plasmid

    #136367

    Purpose

    Expresses epitope-tagged human TET3 R752C variant in mammalian cells

    Depositor

    Roberto Bonasio

    Article

    Beck et al Am J Hum Genet. 2020 Jan 3. pii: S0002-9

    Insert

    TET3 (TET3 Human)
    

    Tags

    2xStrepTagII, FLAG, and HA

    Expression

    Mammalian

    Mutation

    R752C

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pINTO-N3::hTET3 F1072C

    Plasmid

    #136366

    Purpose

    Expresses epitope-tagged human TET3 F1072C variant in mammalian cells

    Depositor

    Roberto Bonasio

    Article

    Beck et al Am J Hum Genet. 2020 Jan 3. pii: S0002-9

    Insert

    TET3 (TET3 Human)
    

    Tags

    2xStrepTagII, FLAG, and HA

    Expression

    Mammalian

    Mutation

    F1072C

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLY57

    Plasmid

    #130926

    Purpose

    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA5B5 with sfgfp::ASV).

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Inserts

    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    

    Use

    Synthetic Biology

    Tags

    ASV tag and pspFΔHTH::λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Anderson promoter: J23106, PpspA-LEA5B5, and Ptet

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLY56

    Plasmid

    #130925

    Purpose

    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA4B4 with sfgfp::ASV).

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Inserts

    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    

    Use

    Synthetic Biology

    Tags

    ASV tag and pspFΔHTH::λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Anderson promoter: J23106, PpspA-LEA4B4, and Ptet

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLY55

    Plasmid

    #130924

    Purpose

    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA3B3 with sfgfp::ASV).

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Inserts

    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    

    Use

    Synthetic Biology

    Tags

    ASV tag and pspFΔHTH::λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Anderson promoter: J23106, PpspA-LEA3B3, and Ptet

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLY53

    Plasmid

    #130911

    Purpose

    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA1B1 with sfgfp::ASV).

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Inserts

    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    

    Use

    Synthetic Biology

    Tags

    ASV tag and pspFΔHTH::λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Anderson promoter: J23106, PpspA-LEA1B1, and Ptet

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mTet1 flox donor

    Plasmid

    #117147

    Purpose

    Making Tet1 conditional knock out

    Depositor

    Jacob Hanna

    Article

    Zviran et al ssrn.3155731

    Insert

    Tet1 (Tet1 Mouse)
    

    Use

    Mouse Targeting

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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