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pk335-CARGO-6mer-35kb-DSF-6-11

Plasmid

#227488

Purpose

6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

Depositor

Joanna Wysocka

Article

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Insert

CARGO to 35kb Downstream Fgf5

Use

CRISPR

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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11_T7-SP-mScarlet3-GPI

Plasmid

#229806

Purpose

In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with a signal peptide and a GPI attachment site

Depositor

Michalis Averof

Article

Karapidaki et al Development. 2026 Apr 15;153(8):dev20541

Insert

SP-mScarlet3-GPI

Use

In vitro transcription

Mutation

T7 promoter

Available Since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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Anti-Kv3.4 K+ channel [N72/11.1R]

Plasmid

#225405

Purpose

Mammalian Expression Plasmid of anti-Kv3.4 K+ channel (Rat) IgG2a R-mAb. Derived from hybridoma N72/11.1.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Kv3.4 K+ channel (Rattus norvegicus) recombinant (Mouse) monoclonal antibody. (Kcnc4 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pOPINO_11-H6

Plasmid

#184280

Purpose

Expresses nanobody H11-H6 in E. coli

Depositor

Ray Owens

Article

Mikolajek et al Proc Natl Acad Sci U S A. 2022 Aug 2;119

Insert

Nanobody H11-H6

Tags

His6

Expression

Bacterial, Insect, and Mamm…

Promoter

T7

Available Since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4.11_Nued2_E1_dAP1-2

Plasmid

#203837

Purpose

Luciferase reporter plasmid for Igf1 enhancer 1 with both AP-1 sites knocked out

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

dAP1-2

Expression

Mammalian

Mutation

KO of both AP1 sites

Available Since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pGL4.11_Nued2_E1

Plasmid

#203830

Purpose

Luciferase reporter plasmid for Igf1 enhancer 1

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

E1

Expression

Mammalian

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pGL4.11_Nued2_E1_dAP1

Plasmid

#203835

Purpose

Luciferase reporter plasmid for Igf1 enhancer 1 containing only the downstream AP1 site

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

dAP1

Expression

Mammalian

Mutation

KO of AP1 upstream

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGL4.11_Nued2_E4

Plasmid

#203833

Purpose

Luciferase reporter plasmid for Igf1 enhancer 4

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

E4

Expression

Mammalian

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGL4.11_Nued2_E5

Plasmid

#203834

Purpose

Luciferase reporter plasmid for Igf1 enhancer 5

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

E5 (Igf1 Mouse)

Expression

Mammalian

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGL4.11_Nued2_E2

Plasmid

#203831

Purpose

Luciferase reporter plasmid for Igf1 enhancer 2

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

E2

Expression

Mammalian

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGL4.11_Nued2_E3

Plasmid

#203832

Purpose

Luciferase reporter plasmid for Igf1 enhancer 3

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

E3

Expression

Mammalian

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGL4.11_Nued2_E1_dAP2

Plasmid

#203836

Purpose

Luciferase reporter plasmid for Igf1 enhancer 1 containing only the upstream AP1 site

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

dAP1

Expression

Mammalian

Mutation

KO of AP1 downstream

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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BASIC_SEVA_69.11

Plasmid

#194214

Purpose

BASIC DNA assembly vector

Depositor

Paul Freemont

Article

Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

Insert

mScarlet counter-selection cassette

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 26, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pGLDV_11 [18e3G]

Plasmid

#199074

Purpose

Even assembly vector with sfGFP dropout for Even level assemblies

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pT2/Col1a1/GFP4_Seq 1.1

Plasmid

#201400

Purpose

Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

Depositor

Maria Castro

Article

Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

Insert

COL1A1

Expression

Mammalian

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-1-Ugi1-11

Plasmid

#197690

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

Ugi1 enhancer-blocking insulator

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-1-Ugi3-11

Plasmid

#197691

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

Ugi3 enhancer-blocking insulator

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-1-Ugi4-11

Plasmid

#197692

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

Ugi4 enhancer-blocking insulator

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-9-NbAct3-11

Plasmid

#197761

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

NbAct3 terminator

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-9-NbHSP-11

Plasmid

#197762

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

NbHSP terminator

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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