We narrowed to 14,429 results for: nts

1. PET-B-HypaSpCas9-NLS-6xHis

   Plasmid

   #207386

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-HypaSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   N692A, M694A, Q695A, H698A, amino acids 1005-1013…

   Promoter

   T7

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. G718A_BattB(GA)_TKterm_AttB(GT)_sGFP-PTEN-IRES-mCherry-P2A-HygroR_noTerm_attP(GA)_IRES-UnaG-2A-Puro-Term

   Plasmid

   #200634

   Purpose

   Plasmid self-excising recombination plasmid for Matreyek Bxb1(GT) landing pad

   Depositor

   Kenneth Matreyek

   Article

   Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

   Insert

   PTEN (PTEN Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV_CaMKII_NES-his-CaMPARI2-F391W-WPRE-SV40

   Plasmid

   #163698

   Purpose

   AAV vector expressing CaMPARI2_F391W primarily in excitatory neurons in rodent cortex

   Depositor

   Gina Turrigiano

   Article

   Trojanowski et al Neuron. 2021 Feb 17;109(4):663-676.e5. d

   Insert

   CaMKII_NES-his-CaMPARI2-F391W-WPRE-SV40
   

   Use

   AAV

   Tags

   FLAG-HA-myc and NES_his

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKII fragment

   Available since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. BCL9-BirA*

   Plasmid

   #109147

   Purpose

   To generate stable cell lines expressing BCL9-BirA* for BioID proximity labelling through co-transfection with pOG44 in Flp-In T-REx host cells

   Depositor

   Mariann Bienz

   Article

   van Tienen et al Elife. 2017 Mar 15;6. doi: 10.7554/eLife

   Insert

   BCL9 (BCL9 Human)
   

   Use

   Tags

   BirA(R118G)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/TetO2

   Available since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. VRQR-HF1 variant

   Plasmid

   #138563

   Purpose

   Expresses human codon-optimized SpCas9-VRQR-HF1 and blasticidin resistance: EFS promoter-VRQR-HF1-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   VRQR-HF1
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335…

   Promoter

   EFS

   Available since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDisplay-EGFP-2A-HA-Nrxn3-alpha- 2(-)/3(+)/4(+)/6(+)

   Plasmid

   #184334

   Purpose

   Expression of mouse Nrx3-alpha lacking insertion at the alternative splice site 4 together with EGFP protein separated by 2A peptide. Contains insertions at alternatively splice sites 1,2,3 and 6.

   Depositor

   Peter Scheiffele

   Article

   Hauser et al Neuron. 2022 May 8. pii: S0896-6273(22)0

   Insert

   Nrxn3-alpha (Nrxn3 Mouse)
   

   Use

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Pb-TRE3G-TYR-HygR

   Plasmid

   #195508

   Purpose

   inducible Piggybac vector expressing human TYR CDS with hygromycin selection

   Depositor

   Joanna Wysocka

   Article

   Bajpai et al Science. 2023 Aug 11;381(6658):eade6289.

   Insert

   TYR (TYR Human)
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFastBac HTB DPP9S D808N

   Plasmid

   #169947

   Purpose

   Purification of catalytically-dead DPP9S from insect cells

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   DPP9 (DPP9 Human)
   

   Use

   Tags

   HIS-TEV

   Expression

   Insect

   Mutation

   D808N

   Promoter

   polyhedrin

   Available since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSNR52-sgTET

   Plasmid

   #46923

   Purpose

   Yeast CEN/ARS vector (Ura3) that contains sgRNA controlled by SNR 52 promoter, targeting endogenous TRE elements of pTET07 promoter

   Depositor

   Jonathan Weissman, Stanley Qi

   Article

   Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

   Insert

   sgRNA targeting endogenous TRE elements of pTET07 promoter
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SNR52

   Available since

   Oct. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC19-E6MBW6

    Plasmid

    #103102

    Purpose

    Cas9 coding gene template with optimized sequence for human codon usage

    Depositor

    Duhee Bang

    Article

    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

    Insert

    E6MBW6(Cas9 coding gene from Staphylococcus lugdunensis M23590)
    

    Use

    CRISPR; Cloning vector

    Tags

    Expression

    Mutation

    human codon-optimized

    Promoter

    Available since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAG8H-EWS_1-264

    Plasmid

    #180467

    Purpose

    Expresses the construct His8-EWS_1-264 with a TEV cleavage site for the removal of the His8 tag

    Depositor

    David Libich

    Article

    Johnson et al Biomol NMR Assign. 2022 Apr;16(1):67-73.

    Insert

    EWSR1 (EWSR1 Human)
    

    Use

    Tags

    His8

    Expression

    Bacterial

    Mutation

    deleted 265-656

    Promoter

    T7

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJS699

    Plasmid

    #168779

    Purpose

    Encodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) and can be combined with pJS697 through homologous recombination

    Depositor

    Timothy Whitehead

    Article

    Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

    Insert

    Spike RBD (S betacoronavirus)
    

    Use

    Tags

    Cmyc

    Expression

    Mutation

    N343Q

    Promoter

    GAL1

    Available since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. 303Gal-FUS

    Plasmid

    #29614

    Depositor

    Aaron Gitler

    Article

    Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 18, 2011

    Availability

    Academic Institutions and Nonprofits only

14. PV225

    Plasmid

    #132334

    Purpose

    dCas9 plant transcriptional activator (AT-CBF1) linked constitutive expression cassette for Zea mays

    Depositor

    Joshua Young

    Article

    Young et al Commun Biol. 2019 Oct 18;2:383. doi: 10.

    Insert

    dCas9-AT-CBF1 (CBF1 Cas9 (Streptococcus pyogenes); AT-CBF1 (Arabidopsis thaliana))
    

    Use

    Tags

    AT-CBF1

    Expression

    Plant

    Mutation

    Codon optimize for expression and stability in Ze…

    Promoter

    Available since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTE4560

    Plasmid

    #107526

    Purpose

    Expresses As crRNA and mCherry in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Inserts

    As crRNA
    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and human U6

    Available since

    April 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRF+275Dux4

    Plasmid

    #21293

    Depositor

    Stephen Tapscott

    Article

    Snider et al Hum Mol Genet. 2009 Jul 1;18(13):2414-30

    Insert

    DUX 4 (DUX4 Human, DUX 4)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

17. pRC/CMV-TYK2P1104A-VSV

    Plasmid

    #139350

    Purpose

    Expression of the TYK2-P1104A variant, rs34536443

    Depositor

    Sandra Pellegrini

    Article

    Li et al J Immunol. 2013 Mar 1;190(5):2335-44. do

    Insert

    TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
    

    Use

    Tags

    VSV

    Expression

    Mammalian

    Mutation

    V362F-P1104A

    Promoter

    CMV

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR221 NLRP1 A66V

    Plasmid

    #166817

    Purpose

    Gateway pDONR vector encoding NLRP1 with the patient mutation A66V (Zhong et al., 2016) (no stop codon)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Expression

    Mutation

    A66V

    Promoter

    Available since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28-p53 (1-73) (72P)

    Plasmid

    #62083

    Purpose

    Expression of human p53 (1-73) (72P) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Vise et al Nucleic Acids Res. 2005 Apr 11;33(7):206

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    HIs

    Expression

    Bacterial

    Mutation

    contains TAD residues 1-73; 72P

    Promoter

    T7

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pYPQ262m

    Plasmid

    #161522

    Purpose

    Gateway entry clone (attL1 & attR5) for CRISPR-zSpCas9 ABE7.10 mediated A-G base editing

    Depositor

    Yiping Qi

    Article

    Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41

    Insert

    TadA(wt)-TadA(7.10)-zSpCas9(D10A)
    

    Use

    CRISPR; Gateway compatible tada(wt)-tada(7.10)-zs…

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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