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pTHY_P0020_T2c_BCD_0.2K

Plasmid

#200056

Purpose

Bicistronic RBS 0.2K Part

Depositor

Ross Thyer

Article

Jansen et al ACS Synth Biol. 2023 Jun 16;12(6):1608-1

Insert

BCD 0.2K

Expression

Bacterial

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pTHY_P0026_T2c_BCD_8.5K

Plasmid

#200050

Purpose

Bicistronic RBS 8.5K Part

Depositor

Ross Thyer

Article

Jansen et al ACS Synth Biol. 2023 Jun 16;12(6):1608-1

Insert

BCD 8.5K

Use

Synthetic Biology

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-pnRFP

Plasmid

#201678

Purpose

Bacteria protein expression of pnRFP biosensor

Depositor

Huiwang Ai

Article

Pang et al ACS Chem Biol. 2023 Jun 16;18(6):1388-13

Insert

pnRFP

Tags

6xHis tag

Expression

Bacterial

Available Since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pAcBAC3-POLY-pnRFP

Plasmid

#201680

Purpose

Boost the expression of pnRFP in mammalian cells (e.g., macrophage RAW 264.7 cells)

Depositor

Huiwang Ai

Article

Pang et al ACS Chem Biol. 2023 Jun 16;18(6):1388-13

Insert

813

Expression

Mammalian

Available Since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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NK621

Plasmid

#196405

Purpose

Strong expression of firefly luciferase codon-optimized for S. rosetta

Depositor

Nicole King

Article

Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092

Insert

Sro5actin-firefly-Sro3actin

Use

Luciferase

Mutation

N/A (synthetic)

Available Since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-Ppyr9eh

Plasmid

#201443

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

Ppyr9eh

Use

Unspecified

Promoter

read through

Available Since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-mNG:Ppyr9eh

Plasmid

#201441

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mNG:Ppyr9eh

Use

Unspecified

Promoter

read through

Available Since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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Atg20-24 pST39

Plasmid

#200439

Purpose

His6 tagged Atg20 and untagged Atg24 from S. cerevisiae for co-expression in E. coli.

Depositor

Michael Ragusa

Article

Reinhart et al Biochemistry. 2022 Apr 14. doi: 10.1021/

Inserts

Atg20
Atg24

Tags

6xHis-TEV

Expression

Bacterial

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv6-puro-3Ty:mScarletI:3Ty

Plasmid

#201093

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mScaret-I

Use

Unspecified

Promoter

read through

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOTv6-hygro-3Ty:mScarletI:3Ty

Plasmid

#201092

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mScaret-I

Use

Unspecified

Promoter

read through

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOTv6-g418-His:mScarletI:His

Plasmid

#201098

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mScarlet-I

Use

Unspecified

Promoter

read through

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv6-g418-His:mNG:His

Plasmid

#201095

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mNeonGreen

Use

Unspecified

Promoter

read through

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOTv6-g418-3Ty:mScarletI:3Ty

Plasmid

#201091

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mScaret-I

Use

Unspecified

Promoter

read through

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOTv6-hygro-3Ty:tagRFPt:3Ty

Plasmid

#201088

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

tagRFPt

Use

Unspecified

Promoter

read through

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOTv6-g418-3Ty:tagRFPt:3Ty

Plasmid

#201087

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

tagRFPt

Use

Unspecified

Promoter

read through

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOTv6-blast-3Ty:tagRFPt:3Ty

Plasmid

#201086

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

tagRFPt

Use

Unspecified

Promoter

read through

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOTv7-g418-tagRFPt

Plasmid

#201085

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

tagRFPt

Use

Unspecified

Promoter

read through

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOTv7-hygro-tagRFPt

Plasmid

#201084

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

tagRFPt

Use

Unspecified

Promoter

read through

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTHY_P0023_T2c_BCD_1.5K

Plasmid

#200053

Purpose

Bicistronic RBS 1.5K Part

Depositor

Ross Thyer

Article

Jansen et al ACS Synth Biol. 2023 Jun 16;12(6):1608-1

Insert

BCD 1.5K

Expression

Bacterial

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pGL2_175

Plasmid

#199107

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

Dummy ORI plus NGS barcode 23, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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