We narrowed to 19,051 results for: Tec;

1. pDEST_FLAG-HUWE1_UBA-mut

   Plasmid

   #187146

   Purpose

   mammalian cell expression of FLAG-HUWE1 M1328A/F1330A

   Depositor

   Eric Fischer

   Article

   Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

   Insert

   HUWE1 (HUWE1 Human)
   

   Tags

   1xFLAG

   Expression

   Mammalian

   Mutation

   M1328A/F1330A

   Available Since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET32a-Spike-S-4P

   Plasmid

   #165094

   Purpose

   protein expression in E. coli

   Depositor

   Rick Tarleton

   Article

   Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

   Insert

   Severe acute respiratory syndrome coronavirus 2 spike protein site 550-570, 785- 805, 810-830 and 1146 1166 (S SARS-CoV2)
   

   Expression

   Bacterial

   Available Since

   March 2, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pINTO-NSA::hSNRNP70

   Plasmid

   #61726

   Purpose

   Expression of human SNRNP70 fused to monomeric streptavidin (N-terminus)

   Depositor

   Roberto Bonasio

   Article

   Beck et al J Proteome Res. 2014 Dec 5;13(12):6135-4

   Insert

   SNRP70 (SNRNP70 Human)
   

   Tags

   mSA (monomeric streptavidin)

   Expression

   Mammalian

   Mutation

   Two silent mutations to ensure restriction site c…

   Promoter

   T-REx (CMV + 2xTetO2)

   Available Since

   Jan. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pYB-Dual(mNG(yeast-opt))

   Plasmid

   #216727

   Purpose

   Dual-species protein expression vector for high-level and inducible protein expression of mNeonGreen (codon optimized for yeast) in both yeast and bacteria from the same construct.

   Depositor

   Emil Jensen

   Article

   Waneskog et al Biotechnol Prog. 2024 May 17:e3482. doi:

   Insert

   mNeonGreen
   

   Expression

   Bacterial and Yeast

   Promoter

   pGAL1(yeast) and pT7-RNAP(bacteria)

   Available Since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Polymerase expression - pCMV-Phi29 (LM2708)

   Plasmid

   #208965

   Purpose

   Unfused Phi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   Phi29-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   Phi29(-exo;D169A)

   Promoter

   CMV and T7

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET-pro-siRNA-V2-TP53

   Plasmid

   #111089

   Purpose

   pET-pro-siRNA-V2 based plasmid for production of recombinant siRNAs (pro-siRNAs) against human TP53 gene.

   Depositor

   Linfeng HUANG

   Article

   Kaur et al Biotechnol Bioeng. 2018 Jun 5. doi: 10.1

   Insert

   TP53 (TP53 Human)
   

   Expression

   Bacterial

   Available Since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV-hA3A-BE3-Y132D

   Plasmid

   #113425

   Purpose

   Expresses hA3A-BE3-Y132D in mammalian cells

   Depositor

   Jia Chen

   Article

   Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

   Insert

   hA3A-BE3-Y132D (APOBEC3A Human, S. pyogenes and Bacteriophage PBS2)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   hAPOBEC3A_Y132D

   Promoter

   CMV

   Available Since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCALNL-Kir2.1

   Plasmid

   #125578

   Purpose

   Cre-dependent expression of Kir2.1 (FLAG-tagged) in mammalian cells.

   Depositor

   Takeshi Imai

   Article

   Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

   Insert

   Kir2.1 (Kcnj2 Mouse)
   

   Use

   Cre/Lox

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   July 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA-Flag-LmoCas8b2

   Plasmid

   #126482

   Purpose

   Encodes L.monocytogenes CRISPR Type I-B Cas8b2 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

   Insert

   LmoCas8b2
   

   Use

   CRISPR

   Tags

   Flag, NLS

   Expression

   Mammalian

   Promoter

   CMV and U6

   Available Since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA-Flag-LmoCas7

    Plasmid

    #126483

    Purpose

    Encodes L.monocytogenes CRISPR Type I-B Cas7 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    LmoCas7
    

    Use

    CRISPR

    Tags

    Flag, NLS

    Expression

    Mammalian

    Promoter

    CMV and U6

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA-Flag-LmoCas5

    Plasmid

    #126484

    Purpose

    Encodes L.monocytogenes CRISPR Type I-B Cas5 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    LmoCas5
    

    Use

    CRISPR

    Tags

    Flag, NLS

    Expression

    Mammalian

    Promoter

    CMV and U6

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1 GW-mCit-PA

    Plasmid

    #113449

    Purpose

    ProteinA-mCitrine Gateway shuttle vector for C-terminal fusions

    Depositor

    Erich Wanker

    Article

    Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

    Type

    Empty backbone

    Use

    Gateway shuttle vector

    Tags

    mCitrine-ProteinA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. B*58:02:01

    Plasmid

    #203262

    Purpose

    Overexpress HLA allele

    Depositor

    Derin Keskin, Catherine Wu

    Article

    Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

    Insert

    B*58:02:01 (HLA-B Human)
    

    Expression

    Mammalian

    Available Since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCDNA_B2AR-q-pep

    Plasmid

    #47437

    Purpose

    FRET sensor for beta2-adrenergic receptor

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Malik et al J Biol Chem. 2013 Jun 14;288(24):17167-7

    Insert

    beta2-adrenergic receptor (ADRB2 Human)
    

    Tags

    10 nm ER/K helix, HA, mCerulean, mCitrine, and q-…

    Expression

    Mammalian

    Promoter

    pCMV

    Available Since

    Sept. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pVC297 VEGF Site#1

    Plasmid

    #47505

    Purpose

    human gRNA expression vector targeting VEGF

    Depositor

    Keith Joung

    Article

    Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

    Insert

    gRNA-VEGF site 1 (VEGFA Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. TRIM27 gRNA (BRDN0001145893)

    Plasmid

    #77516

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TRIM27

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TRIM27 (TRIM27 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ROCK2 gRNA (BRDN0001147800)

    Plasmid

    #76349

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ROCK2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    ROCK2 (ROCK2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV-mA1-BE3

    Plasmid

    #113421

    Purpose

    Expresses mA1-BE3 in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

    Insert

    mA1-BE3 (Apobec1 S. pyogenes and Bacteriophage PBS2, Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pYPQ265G2

    Plasmid

    #164723

    Purpose

    Gateway entry clone (attL1 & attR5) A3A/Y130F-iSpyMacCas9-CBE_V01 for C-T base editing

    Depositor

    Yiping Qi

    Article

    Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

    Insert

    A3A(Y130F)-ziSpyMac(D10A)-UGI)
    

    Use

    CRISPR; Gateway compatible a3a/y130f-ispymaccas9-…

    Expression

    Plant

    Available Since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pVC228 VEGF Site#3

    Plasmid

    #47507

    Purpose

    human gRNA expression vector targeting VEGF

    Depositor

    Keith Joung

    Article

    Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

    Insert

    gRNA-VEGF site 3 (VEGFA Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge