We narrowed to 59,446 results for: cloning

1. pENTR-TER+-shLKB1-Ms

   Plasmid

   #61234

   Purpose

   Entry clone encoding shRNA to mouse LKB1

   Depositor

   Reuben Shaw

   Article

   Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

   Insert

   shLkb1 (Ms/Hu) (STK11, Stk11 Human, Mouse)
   

   Use

   Entr clone

   Promoter

   H1/TO

   Available Since

   Jan. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pAPcrRNA_Eco

   Plasmid

   #106276

   Purpose

   E.coli CRISPR Type I-E crRNA cloning vector for mammalian expression driven by U6 promoter

   Depositor

   Charles Gersbach

   Article

   Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. kank3_pJet1.2

   Plasmid

   #121981

   Purpose

   for generating kank3 riboprobes for WISH in zebrafish

   Depositor

   GuangJun Zhang

   Article

   Hensley et al Sci Rep. 2016 Jun 13;6:27816. doi: 10.10

   Insert

   kank3 (kank3 Zebrafish)
   

   Use

   Cloning vector

   Promoter

   T7

   Available Since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. FB012

   Plasmid

   #119709

   Purpose

   Complete TU for hygromicin resistance in fungi, domesticated into pUPD2 with AATG/GCTT barcodes. Used for gene KO with dual selection, according to FungalBraid modular DNA assembly for ATMT

   Depositor

   Jose Marcos

   Article

   Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

   Insert

   PtrpC:hph:Ttub
   

   Use

   Synthetic Biology; Domestication of dna parts for…

   Available Since

   March 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCELF PAI-RBP v3

   Plasmid

   #45507

   Depositor

   Thomas Gelehrter

   Article

   Heaton et al J Biol Chem. 2001 Feb 2;276(5):3341-7. E

   Insert

   PAI-RBP v3 (SERBP1 Human, Rat)
   

   Tags

   GST tag

   Expression

   Mammalian

   Available Since

   June 20, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pCELF PAI-RBP v2

   Plasmid

   #45505

   Depositor

   Thomas Gelehrter

   Article

   Heaton et al J Biol Chem. 2001 Feb 2;276(5):3341-7. E

   Insert

   PAI-RBP v2 (SERBP1 Human, Rat)
   

   Tags

   GST tag

   Expression

   Mammalian

   Available Since

   June 20, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pQTEV-C9orf58

   Plasmid

   #34819

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

   Insert

   C9orf58 (AIF1L Human)
   

   Tags

   His and TEV

   Expression

   Bacterial

   Available Since

   March 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. KHBD00416

   Plasmid

   #34523

   Depositor

   Bart Deplancke

   Article

   Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

   Insert

   CG17950 (HmgD Fly)
   

   Use

   Gateway donor vector

   Available Since

   Jan. 24, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA5/FRT/TO GFP DNAJB10

   Plasmid

   #19507

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   DNAJB10 (DNAJB2 Mouse)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Oct. 7, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCSDest2-2XNLS-SpCas9-WT-NLS-3XHA-NLS-TALentry

    Plasmid

    #69232

    Purpose

    Wild type SpCas9 expression plasmid to clone TALEs through BbsI site (generates compatible overhangs with Acc65I and BamHI sites)

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    2X NLS, BbsI TALE entry cassette, and NLS-3XHA-NL…

    Expression

    Mammalian

    Available Since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5/FRT/TO HIS DNAJB10

    Plasmid

    #19560

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DNAJB10 (DNAJB2 Mouse)
    

    Tags

    His

    Expression

    Mammalian

    Available Since

    Oct. 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5/FRT/TO DNAJB10

    Plasmid

    #19479

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DNAJB10 (DNAJB2 Mouse)
    

    Expression

    Mammalian

    Available Since

    Oct. 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMDC32B-B/c

    Plasmid

    #227963

    Purpose

    Expression plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.

    Depositor

    Alberto Carbonell

    Article

    Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

    Insert

    B/c
    

    Expression

    Plant

    Promoter

    35S

    Available Since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFNC-5

    Plasmid

    #233297

    Purpose

    Plasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. TcR, easily curable via sucrose counterselection.

    Depositor

    Pablo Ivan Nikel

    Article

    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

    Inserts

    BxbI phage integrase
    sacB
    

    Expression

    Bacterial

    Promoter

    PEM7 and unknown

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNDGG010

    Plasmid

    #231315

    Purpose

    Vector part for BEVA; BEVA2.0 Position 4 attB recombinase site, AmpR. Also includes FRT EF module for L1 GG cloning.

    Depositor

    Barney Geddes

    Article

    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

    Insert

    BEVA2.0 Position 4 attB recombinase site, AmpR. Also includes FRT EF module for L1 GG cloning.
    

    Expression

    Bacterial

    Available Since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNDGG022

    Plasmid

    #231306

    Purpose

    Vector part for BEVA; BEVA2.0 Position 1 Level 1 BsaI cloning site with alternate CAGA 3' fusion site, without T0, AmpR

    Depositor

    Barney Geddes

    Article

    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

    Insert

    BEVA2.0 Position 1 Level 1 BsaI cloning site with alternate CAGA 3' fusion site, without T0, AmpR
    

    Expression

    Bacterial

    Available Since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNDGG009

    Plasmid

    #231314

    Purpose

    Vector part for BEVA; BEVA2.0 Position 4 FRT recombinase site, AmpR. Also includes attB EF module for L1 GG cloning.

    Depositor

    Barney Geddes

    Article

    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

    Insert

    BEVA2.0 Position 4 FRT recombinase site, AmpR. Also includes attB EF module for L1 GG cloning.
    

    Expression

    Bacterial

    Available Since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFNC-6

    Plasmid

    #227669

    Purpose

    Plasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. GmR, easily curable via sucrose counterselection.

    Depositor

    Pablo Ivan Nikel

    Article

    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

    Inserts

    BxbI phage integrase
    sacB
    

    Expression

    Bacterial

    Promoter

    PEM7

    Available Since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR221-DXO

    Plasmid

    #177992

    Purpose

    For gateway cloning of DXO

    Depositor

    Karla Feijs

    Article

    Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

    Insert

    DXO (DXO Human)
    

    Available Since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDONR221-PARP11

    Plasmid

    #184469

    Purpose

    For gateway cloning of PARP11

    Depositor

    Karla Feijs

    Article

    Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

    Insert

    PARP11 (PARP11 Human)
    

    Available Since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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