We narrowed to 14,283 results for: cas9
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Tol2-LyzC-Rac-WT(guide resistant)-2a-mcherry-U6ac-Rac guides
Plasmid#168242Purpose"Rescue Rac expression in neutrophil specific knockout"DepositorInsertRac(guide resistant)-WT
UseZebrafish expressionTagsmcherryPromoterLyzCAvailable SinceMarch 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTR(EF)-tRNA(Q)
Plasmid#170517PurposePCR template vector for amplifying gRNAcore(EF)-tRNA(Q) fragment; used together with pAC-U63-tgRNA-nlsBFP or pAC-U63-tgRNA-Gal80DepositorInsertgRNAcore(EF)-tRNA(Q)
UsePcr template vector for amplifying grnacore(ef)-t…Available SinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAT-9208
Plasmid#124221PurposeCloning plasmid for a self-targeting gRNA libraryDepositorInsertself-targeting gRNA
UseCRISPRExpressionBacterialPromoterJ23119Available SinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPTG-GG-sg2+sg3
Plasmid#127199PurposePlasmid expressing sgRNA2 (TACCACATTTGTAGAGGTT) & sgRNA3 (CAATGTATCTTATCATGTC) as polycistronic tRNA-gRNA from single U6 promoterDepositorInserttRNA-gRNA
UseEmpty sgrna plasmidExpressionMammalianPromoterU6Available SinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pORANGE GFP-Cacnb1 KI
Plasmid#139660PurposeEndogenous tagging of CaVβ1: N-terminal (amino acid position: S8)DepositorInsertgRNA and GFP donor
ExpressionMammalianPromoterU6 and CbhAvailable SinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pORANGE Cacnb2-GFP KI
Plasmid#139661PurposeEndogenous tagging of CaVβ2: C-terminal (amino acid position: Q655)DepositorInsertgRNA and GFP donor
ExpressionMammalianPromoterU6 and CbhAvailable SinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBS-PtH
Plasmid#133391PurposePiggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR)DepositorTypeEmpty backboneExpressionMammalianAvailable SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBS-BtH
Plasmid#133392PurposePiggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR)DepositorTypeEmpty backboneExpressionMammalianAvailable SinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
8xCTS1_delta-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro_SV40polyA
Plasmid#124540PurposeCloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.DepositorInsertdelta-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro
ExpressionMammalianMutationd11-41, d11-41PromoterMinimal ADEAvailable SinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
8xCTS1_deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro_SV40polyA
Plasmid#124538PurposeCloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.DepositorInsertdeltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro
ExpressionMammalianMutationd11-41 C55G, d11-41PromoterMinimal ADEAvailable SinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
8xCTS1_BpiI-cloning-site_SV40polyA
Plasmid#124547PurposeDestination cloning vector for tRNA-flanked sgRNA. A BpiI placeholder is present between a minimal ADE promoter and SV40 polyA sequence.DepositorTypeEmpty backboneExpressionMammalianAvailable SinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV CMV-driven AcrIIA4-scaffold (2xBsmBI sites)
Plasmid#120297PurposeAAV vector for expression of AcrIIA4 (no miR binding sites, control vector)DepositorInsertAcrIIA4
UseAAV and CRISPRTagsFLAGExpressionMammalianAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV CMV-driven AcrIIA4-2xmiR-122 target sites
Plasmid#120298PurposeAAV vector for expression of AcrIIA4 with two miR-122 binding sitesDepositorInsertAcrIIA4-2xmiR-122 binding sites
UseAAV and CRISPRTagsFLAGExpressionMammalianAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV CMV-driven AcrIIC3-scaffold (2xBsmBI sites)
Plasmid#120301PurposeAAV vector for expression of AcrIIC3 (no miR binding sites, control vector)DepositorInsertAcrIIC3
UseAAV and CRISPRExpressionMammalianAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONOR-PARK6e5-I368N
Plasmid#86154PurposeDonor plasmid for PARK6 exon5 I368N sequence. Also contains TagBFP and dTomatoDepositorInsertPINK1 exon 5 homology arms (PINK1 Human)
ExpressionBacterial and MammalianAvailable SinceJune 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONOR-SNCAe3-WT
Plasmid#85847PurposeDonor plasmid for SNCA exon3 wild type sequence. lso contains TagBFP and dTomatoDepositorInsertSNCA exon 3 homology arms (SNCA Human)
ExpressionBacterial and MammalianAvailable SinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
1184_pAAV-U6-ApoB-gRNA2-CB-EmGFP
Plasmid#89062PurposeAAV-gRNA targeting the murine Apob geneDepositorInsertEmGFP
UseAAV and CRISPRExpressionMammalianPromoterChicken beta actin with partial CMV enhancerAvailable SinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
non-targeting control gRNA (BRDN0001147929)
Plasmid#80254Purpose3rd generation lentiviral gRNA plasmid, non-targeting control gRNADepositorInsertNon-targeting control gRNA
UseCRISPR and LentiviralExpressionMammalianPromoterhU6Available SinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
non-targeting control gRNA (BRDN0001147380)
Plasmid#80225Purpose3rd generation lentiviral gRNA plasmid, non-targeting control gRNADepositorInsertNon-targeting control gRNA
UseCRISPR and LentiviralExpressionMammalianPromoterhU6Available SinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only
