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Plasmid#59892Purposesynthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene with two seed sites mutatedDepositorInsertmCherry with intron containing the mouse mir-124–3
UseTagsPEST and VAMP 3 UTRExpressionMammalianMutationVAMP 3 UTR--3rd and fourth seed site removedPromoterdoxycycline (Dox)-inducible promoter (CMV/TO)Available sinceApril 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
sgFFL_d3
Plasmid#59891Purposesynthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene with one seed site mutated.DepositorInsertmCherry with intron containing the mouse mir-124–3
UseTagsPEST and VAMP 3 UTRExpressionMammalianMutationVAMP 3 UTR--3rd seed site removedPromoterdoxycycline (Dox)-inducible promoter (CMV/TO)Available sinceApril 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pg-HIF-1alpha-EGFP
Plasmid#87204PurposeC-terminally EGFP-tagged human HIF-1aDepositorInsertHIF-1a (HIF1A Human)
UseTagsEGFPExpressionMammalianMutationL40F, M88V, Q814RPromoterCMVAvailable sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO_FOXA1_#1
Plasmid#70095PurposeExpression of shRNA to human FOXA1, puromycin selectionDepositorInsertFOXA1 (FOXA1 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6Available sinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLKO_FOXA1_#2
Plasmid#70096PurposeExpression of shRNA to human FOXA1, puromycin selectionDepositorInsertFOXA1 (FOXA1 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoteru6Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
FLAG-PRMT6(V86K/D88A)
Plasmid#164698PurposeExpression of PRMT6(V86K/D88A) with an N-terminal FLAG tagDepositorInsertPRMT6 (PRMT6 Human)
UseTagsFLAGExpressionMammalianMutationV86K, D88APromoterCMVAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDYT001
Plasmid#186549PurposeLineage tracing vector (with barcoded Target Sites and triple sgRNAs)DepositorInsert14-bp random integration barcode and three target sites and 3x sgRNAs
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pg-ODD-EGFP
Plasmid#87211PurposeHIF-1a's ODD domain fused to EGFPDepositorInsertHIF-1a ODD domain (HIF1A Human)
UseTagsEGFPExpressionMammalianMutationODD domain (AA 530-652)PromoterCMVAvailable sinceMarch 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV-IRES_Tomato
Plasmid#107229PurposeTomato expressing plasmidDepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
MCP-MSN
Plasmid#210704PurposeThis plasmid express EF1a promoter driven MCP-MSN transactivation moduleDepositorInsertMCP fused to transactivation domain from Human transcription factor MRTF-A, STAT-1 and NRF2
UseLentiviralTagsEGFPExpressionMammalianMutationN55K in MCPPromoterEF1aAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMH0006
Plasmid#135448PurposeHuman expression vector containing Ubiquitous Chromatin Opening Element (UCOE) upstream of EF1alpha promoter, dCas9 that is fused to NLS, tagBFP and a KRAB domain.DepositorInsertdCas9-BFP-KRAB
UseLentiviralTagstagBFPExpressionMammalianMutationPromoterEF1AAvailable sinceJuly 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEv-Fat4-citrine
Plasmid#112854PurposeConstitutive expression of human fat4 protein fused to citrine fluorescent proteinDepositorInsertFAT4 (FAT4 Human)
UseTagsCitrineExpressionMammalianMutationPromoterCMVAvailable sinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-mKOR
Plasmid#179285PurposeExpress mouse kappa opioid receptor (GFP tagged) in mammalian cellsDepositorInsertkappa opioid receptor (Oprk1 Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLX302_HOXB13
Plasmid#70089PurposeExpression of human HOXB13 from CMV promoter, puromycin selection markerDepositorInsertHOXB13 (HOXB13 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 HA-PHLPP1 full length
Plasmid#37100PurposeExpresses HA tagged, full-length human PHLPP1 in mammalian cellsDepositorInsertPHLPP1 (PHLPP1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
MCP-eN3x9
Plasmid#210706PurposeThis plasmid express EF1a promoter driven MCP-eN3x9 transactivation moduleDepositorInsertMCP fused to transactivation domain comprising eNRF2 and 3x 9aa Transactivation domain
UseLentiviralTagsEGFPExpressionMammalianMutationN55K in MCPPromoterEF1aAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Myc-AIM2
Plasmid#73958PurposeExpress c-Myc-tagged human AIM2 in mammalian cellsDepositorInsertabsent in melanoma 2 (AIM2) (AIM2 Human)
UseTagsc-mycExpressionMammalianMutationPromoterCMV iEAvailable sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSLIK 3xFLAG-MAVS hygro
Plasmid#158634PurposeLentiviral expression vector for an inducible 3xFLAG-tagged MAVS constructDepositorInsertMAVS (MAVS Human)
UseLentiviralTags3XFLAGExpressionMutationPromoterAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
AA0250-mCherry-2a-hM4D-nrxn1a rev
Plasmid#60544PurposeCo-expresses hM4Dnrxn and a red fluorescent label from a Cre-dependent virusDepositorInsertsUseAAV and Cre/LoxTags2xHAExpressionMutationPromoterCAGAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHR EGFR-CD3ε-FusionRed
Plasmid#188626PurposeHuman EGFR labeled with CD3ε ITAMs for monitoring with CD3ε/ZtSH2 pYtag biosensorDepositorInsertEGFR (EGFR Synthetic, Human)
UseLentiviralTagsCD3ε-FusionRedExpressionMammalianMutationPromoterSFFVAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
MCP-NMS
Plasmid#210705PurposeThis plasmid express EF1a promoter driven MCP-NMS transactivation moduleDepositorInsertMCP fused to transactivation domain from Human transcription factor NRF2, MRTF-A and STAT1
UseLentiviralTagsEGFPExpressionMammalianMutationN55K in MCPPromoterEF1aAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLT 653
Plasmid#59996PurposeExpresses a hybrid dsRNA corresponding to the Caenorhabditis elegans dhc-3/klp-16 genes in bacteria; ingestion of the bacteria by C elegans produces an RNAi response & leads to more male progenyDepositorUseTagsExpressionBacterialMutationpartial genomicPromoterAvailable sinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHR EGFR-SLP76-TagBFP
Plasmid#188630PurposeHuman EGFR labeled with SLP76 for monitoring with SLP76/VISH2 pYtag biosensorDepositorInsertEGFR (EGFR Synthetic, Human)
UseLentiviralTagsSLP76-TagBFPExpressionMammalianMutationPromoterSFFVAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
H2B-GFP-Aurora B WT
Plasmid#184042PurposeExpresses H2B-GFP fused to Aurora B WTDepositorInsertAURKB (AURKB Human)
UseTagsEGFP and H2BExpressionMammalianMutationPromoterCMVAvailable sinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRecLV105
Plasmid#107238PurposeA lentiviral vector for expression in mammalian cellsDepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDYT002
Plasmid#186550PurposeLineage tracing vector (with barcoded Target Sites and triple sgRNAsDepositorInsert14-bp random integration barcode and three target sites
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
6xHis-MBP-TEV-mDvl2PDZ(264-353)_pCDFduet
Plasmid#216386PurposeBacterial expression vector for MBP-tagged mouse Dvl2 PDZ domain (residues 264-353)DepositorInsertDishevelled-2 (Dvl2 Mouse)
UseTags6xHis-MBP-TEVExpressionBacterialMutationPromoterT7Available sinceApril 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
6xHis-MBP-TEV-mDvl2DEP(416-510)_pCDFduet
Plasmid#216387PurposeBacterial expression vector for MBP-tagged mouse Dvl2 DEP domain (residues 416-510)DepositorInsertDishevelled-2 (Dvl2 Mouse)
UseTags6xHis-MBP-TEVExpressionBacterialMutationPromoterT7Available sinceApril 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
FU-mILa-CRW
Plasmid#84556Purposelentiviral expression of mouse IL1a and mRFPDepositorInsertIL1a (Il1a Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC19 EGFRup-CD3ε-mNeonGreen-EGFRdown
Plasmid#188634PurposeHDR template for CD3ε-mNeonGreen knock-in at C-terminus of human EGFRDepositorInsertEGFR (EGFR Synthetic, Human)
UseCRISPRTagsCD3ε-mNeonGreenExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLT 651
Plasmid#59998PurposeExpresses a hybrid dsRNA corresponding to the Caenorhabditis elegans klp-16/him-8 genes in bacteria; ingestion of the bacteria by C elegans produces an RNAi response & leads to more male progenyDepositorUseTagsExpressionBacterialMutationpartial genomicPromoterAvailable sinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
6xHis-TEV-ATG3 (Human autophagy E2-like enzyme)
Plasmid#169079PurposeHuman ATG3DepositorInsertATG3 (ATG3 Human)
UseTags6X Histidine Tag, TEV cleavage siteExpressionBacterialMutationPromoterT7 lac promoterAvailable sinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pg-proPHD2-PHD2-EGFP
Plasmid#87210PurposePHD2-EGFP expressed under the control of the PHD2 promoterDepositorInsertPHD2 (EGLN1 Human)
UseTagsEGFPExpressionMammalianMutationH313NPromoterPHD2Available sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBabepuro3-p18 A61D
Plasmid#24937DepositorInsertp18 (CDKN2C Human)
UseRetroviralTagsExpressionMammalianMutationA61DPromoterAvailable sinceJune 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMIR-Report-Luc-KLF4-FL
Plasmid#34597DepositorInsertKLF4 (KLF4 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSB137 - pL2_pSB90_pNOS::rLUC-I::tNOS_tOCS::fLUC-I::pSTR1
Plasmid#123194Purposebinary plant vector for transient dual luciferase (rLUC & fLUC with introns) expressionDepositorInsertpNOS::rLUC-I::tNOS tOCS::fLUC-I::pSTR1
UseLuciferaseTagsExpressionPlantMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
MCP-3x9aaTAD
Plasmid#210707PurposeThis Plasmid express EF1a promoter driven MCP-3x 9aa transactivation moduleDepositorInsertMCP fused to 3x 9aa transactivation domain derived from MRTF-B and MYOCD1
UseLentiviralTagsEGFPExpressionMammalianMutationN55K in MCPPromoterEF1aAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLX304-MARK3
Plasmid#107235PurposeLentiviral gene expression vector for phospho-MARK3 expressionDepositorInsertMARK3
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3xFLAG-MAVS
Plasmid#158628PurposeGateway entry vector for an inducible 3xFLAG-tagged MAVSDepositorInsertMAVS (MAVS Human)
UseGateway entry vectorTags3XFLAGExpressionMutationPromoterAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only