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pVRa14_1324

Plasmid

#49665

Depositor

Christopher Voigt

Publication

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF14_1324

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pVRa04_1617

Plasmid

#49658

Depositor

Christopher Voigt

Publication

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF04_1617

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV.U6-sasgRNA(SapI)_Ple155-CI-EGFP-W3SL_BbsI(GGA)

Plasmid

#231367

Purpose

Ple155-driven EGFP, also encoding U6-driven sgRNA cassette, with SapI sites to clone crRNA sequence

Depositor

Viviana Gradinaru

Publication

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

EGFP

Use

AAV

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.U6-sasgRNA(SapI)_Ple155-CI-mRuby2-W3SL_BbsI(GGA)

Plasmid

#231368

Purpose

Ple155-driven mRuby2, also encoding U6-driven sgRNA cassette, with SapI sites to clone crRNA sequence

Depositor

Viviana Gradinaru

Publication

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

mRuby2

Use

AAV

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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delCMV-mCitrine

Plasmid

#214144

Purpose

Volume marker / Control sensor

Depositor

Leif Dehmelt

Publication

Nanda et al Nat Commun. 2023 Dec 15;14(1):8356. doi:

Insert

mCitrine

Expression

Mammalian

Promoter

delCMV

Available since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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pHis8-4b-McDAH

Plasmid

#140129

Purpose

Expresses McDAH with N-terminal 8xHis-tag in BL21 E. coli

Depositor

Jing-Ke Weng

Publication

Kim et al Nat Commun. 2020 Apr 20;11(1):1867. doi:

Insert

Dechloroacutumine halogenase from Menispermum canadense

Tags

8x-His, TEV protease site (ENLYFQ)

Expression

Bacterial

Promoter

T7

Available since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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pVRc33_375

Plasmid

#49748

Depositor

Christopher Voigt

Publication

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

AS33_375

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pLux

Available since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pscAAV.T7-SP6-BC(p11-14)-CAG-EGFP-SV40pA-T7

Plasmid

#231350

Purpose

Self complementary AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses EGFP from CAG promoter.

Depositor

Viviana Gradinaru

Publication

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

T7-SP6-BC(p11-14)

Use

AAV

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.T7-SP6-BC(p11-14)-mDLX-minBG-CI-mRuby2-W3SL-T7

Plasmid

#231352

Purpose

Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses mRuby2 from minBG promoter with mDLX enhancer.

Depositor

Viviana Gradinaru

Publication

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

T7-SP6-BC(p11-14)

Use

AAV

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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Ppst93-MCRaeo-NG mcrBGA

Plasmid

#192769

Purpose

Expresses NG-tagged MCRaeoBGA in Methanococcus maripaludis

Depositor

William B. Whitman

Publication

Shao et al Commun Biol. 2022 Oct 20;5(1):1113. doi:

Insert

Methyl coenzyme M reductase

Use

Synthetic Biology; Heterologous expression in met…

Tags

Flag-Strep2

Available since

Dec. 7, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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Ppst93-MCRaeo-NG mcrBCGA

Plasmid

#192768

Purpose

Expresses NG-tagged MCRaeoBCGA in Methanococcus maripaludis

Depositor

William B. Whitman

Publication

Shao et al Commun Biol. 2022 Oct 20;5(1):1113. doi:

Insert

Methyl coenzyme M reductase

Use

Synthetic Biology; Heterologous expression in met…

Tags

Flag-Strep2

Available since

Dec. 7, 2022

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
Ppst93-MCRaeo-NG mcrBDGA

Plasmid

#192767

Purpose

Expresses NG-tagged MCRaeoBDGA in Methanococcus maripaludis

Depositor

William B. Whitman

Publication

Shao et al Commun Biol. 2022 Oct 20;5(1):1113. doi:

Insert

Methyl coenzyme M reductase

Use

Synthetic Biology; Heterologous expression in met…

Tags

Flag-Strep2

Available since

Dec. 7, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pCAG-eSpCas9-2A-GFP-sgRNA-RDH11

Plasmid

#161924

Purpose

To generate RDH11 KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against RDH11 exon 2.

Depositor

Sébastien Britton

Publication

Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

Insert

sgRNA targeting RDH11 exon 2

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0061

Plasmid

#177026

Purpose

Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

Depositor

Nicola Patron

Publication

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

iridoid oxidase; CYP76A26

Use

Synthetic Biology

Mutation

amplified from cDNA, contains T359S relative to K…

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pSUPER.retro.puro-shLARP7

Plasmid

#113537

Purpose

Retroviral vector designed to knock down LARP7.

Depositor

Blerta Xhemalce

Publication

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

shLARP7 (LARP7 Human)

Use

Retroviral

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGEX-5X-1-MePCE_aa470-689

Plasmid

#113544

Purpose

Produces an N-terminal GST-tagged MePCE protein consisting of amino acids 470 - 689.

Depositor

Blerta Xhemalce

Publication

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

MePCE (MEPCE Human)

Tags

GST

Expression

Bacterial

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGEX-5X-1_MePCE_aa432-689

Plasmid

#113543

Purpose

Produces an N-terminal GST-tagged MePCE protein consisting of amino acids 432 - 689.

Depositor

Blerta Xhemalce

Publication

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

MePCE (MEPCE Human)

Tags

GST

Expression

Bacterial

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGEX-5X-1_MePCE_aa400-689

Plasmid

#113542

Purpose

Produces an N-terminal GST-tagged MePCE protein consisting of amino acids 400 - 689.

Depositor

Blerta Xhemalce

Publication

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

MePCE (MEPCE Human)

Tags

GST

Expression

Bacterial

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGEX-5X-1_MePCE_aa1-400

Plasmid

#113541

Purpose

Produces an N-terminal GST-tagged MePCE protein consisting of amino acids 1 - 400.

Depositor

Blerta Xhemalce

Publication

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

MePCE (MEPCE Human)

Tags

GST

Expression

Bacterial

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pVRa31_2963

Plasmid

#49688

Depositor

Christopher Voigt

Publication

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF31_2963

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge