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Showing: 801 - 820 of 9690 results

  3. PX552

    Plasmid
    #60958
    Purpose
    pAAV-U6sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). AAV plasmid for sgRNA cloning. GFP-KASH fusion facilitates FACS sorting of cells and nuclei.
    Depositor
    Feng Zhang
    Article
    Swiech et al Nat Biotechnol. 2014 Oct 19. doi: 10.103
    Inserts
    U6_(SpaI)_sgRNA
    Syn_EGFP-KASH
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Synapsin and U6
    Available since
    Nov. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    60958-plasmid-map-sequence-id-382829

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  6. pFETCh_HHEX

    Plasmid
    #72353
    Purpose
    Donor vector for 3' FLAG tag of human HHEX
    Depositor
    Richard M. Myers, Eric Mendenhall
    Insert
    HHEX Homology Arms (HHEX Human)
    Use
    CRISPR
    Tags
    3XFLAG-P2A-NeoR
    Expression
    Mutation
    Promoter
    Available since
    June 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    72353-plasmid-map-sequence-id-149456

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  7. pDGB3 alpha2 AtUBQ10:LbCas12a:Tnos (GB2479)

    Plasmid
    #160629
    Purpose
    TU for the expression of LbCas12a
    Depositor
    Diego Orzaez
    Insert
    AtUBQ10-:LbCpf1(Cas12a):Tnos
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    UBQ10
    Available since
    March 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    160629-plasmid-map-sequence-id-321990

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  8. Lenti-EF1alpha-dCas9-KRAB_Hygro

    Plasmid
    #192663
    Purpose
    3rd generation lenti vector encoding dCas9-KRAB with 2A Hygro resistance marker
    Depositor
    Jeffrey Miner
    Insert
    dCas9-KRAB
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D10A, D839A, H840A and N863A in Cas9
    Promoter
    EF1a
    Available since
    Dec. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    192663-plasmid-map-sequence-id-379915

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  9. FU_tetO_dCas9-VP192-T2A-EGFP

    Plasmid
    #121173
    Purpose
    Lentiviral construct for inducible expression of dCas9-VP192 transcriptional activator and EGFP.
    Depositor
    Timo Otonkoski
    Insert
    dCas9-VP192-T2A-EGFP
    Use
    CRISPR and Lentiviral; Inducible by doxycycline
    Tags
    T2A-EGFP-ires-puro
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    121173-plasmid-map-sequence-id-234995

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  10. Lenti-EF1alpha-VP64-dCas9-VP64-ZsG

    Plasmid
    #192667
    Purpose
    3rd generation lenti vector encoding VP64-dCas9-VP64 with 2A ZsGreen1
    Depositor
    Jeffrey Miner
    Insert
    VP64-dCas9-VP64, ZsGreen1
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D10A and N863A in Cas9
    Promoter
    EF1a
    Available since
    Dec. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    192667-plasmid-map-sequence-id-379870

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  11. pIVTR(T7A)-Cas9n-RT-T2A-PuroR

    Plasmid
    #172296
    Purpose
    In vitro transcription of Cas9n-RT-T2A-PuroR synthetic construct for PRIME editing
    Depositor
    Patrik Verstreken
    Insert
    Cas9n-RT-T2A-PuroR
    Use
    CRISPR
    Tags
    Expression
    Mutation
    H840A (Cas9)
    Promoter
    T7 promoter A-initiating
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    172296-plasmid-map-sequence-id-340322

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  12. pX330 Mouse 3' Hp1a gRNA

    Plasmid
    #127898
    Purpose
    WT Cas9 Vector targeting the 3' end of the mouse Hp1a gene
    Depositor
    Mark Groudine
    Insert
    gRNA/Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    127898-plasmid-map-sequence-id-251076

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  13. pCDNA3.3 CAS9 IRES-Dsred

    Plasmid
    #129444
    Purpose
    expression of FnCas9 and DsRed
    Depositor
    Oskar Laur
    Insert
    FnCas9 IRES-Dsred
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    129444-plasmid-map-sequence-id-256492

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  14. pX330 Mouse 5' Npm1 gRNA

    Plasmid
    #127900
    Purpose
    WT Cas9 Vector targeting the 5' end of the mouse Npm1 gene
    Depositor
    Mark Groudine
    Insert
    gRNA/Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    127900-plasmid-map-sequence-id-249686

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  15. pCSDC2-donor

    Plasmid
    #113813
    Purpose
    CRISPR donor plasmid to tag human transcription factor CSDC2 with GFP
    Depositor
    Kevin White
    Insert
    CSDC2 homology arms flanking EGFP-IRES-Neo cassette (CSDC2 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    113813-plasmid-map-sequence-id-229910

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  16. pZNF215-donor

    Plasmid
    #113804
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF215 with GFP
    Depositor
    Kevin White
    Insert
    ZNF215 homology arms flanking EGFP-IRES-Neo cassette (ZNF215 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    rs2239733, rs2239732, hg38:11:6956348:T>G, rs1…
    Promoter
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    113804-plasmid-map-sequence-id-231033

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  17. pZNF41-donor

    Plasmid
    #112375
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF41 with GFP
    Depositor
    Kevin White
    Insert
    ZNF41 homology arms flanking EGFP-IRES-Neo cassette (ZNF41 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:X:47447496:G>T, hg38:X:47448269:C>A, h…
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112375-plasmid-map-sequence-id-229739

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  18. pELF4-donor

    Plasmid
    #112371
    Purpose
    CRISPR donor plasmid to tag human transcription factor ELF4 with GFP
    Depositor
    Kevin White
    Insert
    ELF4 homology arms flanking EGFP-IRES-Neo cassette (ELF4 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112371-plasmid-map-sequence-id-229726

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  19. pZBTB26-donor

    Plasmid
    #112356
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZBTB26 with GFP
    Depositor
    Kevin White
    Insert
    ZBTB26 homology arms flanking EGFP-IRES-Neo cassette (ZBTB26 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:9:122919395:A>G, hg38:9:122919458:T>G,…
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112356-plasmid-map-sequence-id-229674

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  20. pZNF84-donor

    Plasmid
    #112330
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF84 with GFP
    Depositor
    Kevin White
    Insert
    ZNF84 homology arms flanking EGFP-IRES-Neo cassette (ZNF84 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    rs623100, hg38:12:133058872:T>C, hg38:12:13305…
    Promoter
    Available since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    112330-plasmid-map-sequence-id-228558

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Showing: 801 - 820 of 9690 results