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Showing: 801 - 820 of 827 results

  6. pLV-RnSyt1-sgRNA3-dCas9-KRAB-TagBFP2 (Identifier AAAA-0247)

    Plasmid
    #202556
    Purpose
    Synaptotagmin-1 sgRNA3 with TagBFP2
    Depositor
    Frederic Meunier
    Article
    Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:
    Insert
    CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ - CACCTCCTCCTGCAGCGGCAGCATCGG) (Syt1 Rat)
    Use
    CRISPR and Lentiviral
    Tags
    3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2
    Expression
    Mutation
    Promoter
    Available since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    202556-plasmid-map-sequence-id-404689

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  7. hNTo1-qgRNA-pYJA5

    Plasmid
    #217781
    Purpose
    Non-targeting control 1 qgRNA-pYJA5 plasmid from the T.spiezzo library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloning
    Depositor
    Adriano Aguzzi
    Article
    Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038
    Insert
    Quadruple sgRNA-expression cassette for nontargeting control for gene ablation
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    human U6, mouse U6, human H1, human 7SK
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    217781-plasmid-map-sequence-id-466019

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  8. hNTa2-qgRNA-pYJA5

    Plasmid
    #217780
    Purpose
    Non-targeting control 2 qgRNA-pYJA5 plasmid from the T.gonfio library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloning
    Depositor
    Adriano Aguzzi
    Article
    Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038
    Insert
    Quadruple sgRNA-expression cassette for nontargeting control for gene activation and epigenetic silencing
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    human U6, mouse U6, human H1, human 7SK
    Available since
    April 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    217780-plasmid-map-sequence-id-469426

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  14. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs11257655-guide3

    Plasmid
    #118202
    Purpose
    CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs11257655 (CDC123/CAMK1D GWAS locus)
    Depositor
    Jorge Ferrer
    Article
    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    hPGK and U6
    Available since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118202-plasmid-map-sequence-id-240385

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  15. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR GLIS3 TSS-guide3

    Plasmid
    #118175
    Purpose
    CRISPR-mediated repression of GLIS3. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.
    Depositor
    Jorge Ferrer
    Article
    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    hPGK and U6
    Available since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118175-plasmid-map-sequence-id-240390

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  16. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR C2CD4AB-enh3-guide3

    Plasmid
    #125410
    Purpose
    CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs17205526 (C2CD4A/B GWAS locus)
    Depositor
    Jorge Ferrer
    Article
    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    hPGK and U6
    Available since
    July 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    125410-plasmid-map-sequence-id-250168

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  18. Musunuru-Cowan TALEN Kit

    Plasmid Kit
    #1000000034
    Purpose
    Used to efficiently assemble TALEN constructs with custom repeat arrays, each containing 15 repeats. Contains pre-assembled trimer and tetramer RVD domains designed for rapid TALEN assembly.
    Depositor
    Kiran Musunuru
    Available since
    July 19, 2013
    Availability
    Academic Institutions and Nonprofits only

  19. Ekker Lab TALEN kit

    Plasmid Kit
    #1000000038
    Purpose
    Contains all 256 possible combinations of pFUS_B4 clones for Golden Gate assembly of 15-RVD TALENs for high-throughput mutagenesis
    Depositor
    Stephen Ekker
    Available since
    March 14, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 801 - 820 of 827 results