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  1. Plasmid Cloning by PCR

    Type
    Blog Post
    ...Forward Primer will use the sequence 5'-ATGTGGCATATCTCGAAGTAC-3' for the region that binds the ORF and...primer, making our Forward Primer 5'-GAATTCATGTGGCATATCTCGAAGTAC-3'. Many restriction enzymes do not cut...final Forward Primer sequence of 5'-TAAGCAGAATTCATGTGGCATATCTCGAAGTAC-3'. For the Reverse Primer, the design...of the ORF, including the stop codon (5'-TGGCATATCTCGAAGTACTGA-3'), then adding NotI (GCGGCCGC) and then.... This gives us a sequence of 5'-TGGCATATCTCGAAGTACTGAGCGGCCGCTAAGCA-3' (30bp with 18bp of homology to...chose for our reverse primer (5’-TGGCATATCTCGAAGTACTGAGCGGCCGCTAAGCA-3’) into this calculator we get a...

  2. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...and the canonical loxP sequence is ATAACTTCGTATA-GCATACAT-TATACGAAGTTAT. The loxP sequence does not occur...

  3. TALENs for Endogenous Zebrafish Genes

    Type
    Collection
    ... apoa4 TAL3022 & TAL3025 TGGCAGGACCAACCAATGcccagcatggacctggTGAAAAATGCTTTCTGGA apoa4-like TAL3023 & TAL3024...CHCHD4 TAL3408 & TAL3409 TCTACCTAATGGCGACATaaactggaactgcccgtGTTTGGGCGGCATGGCCA cish.b-(socs8) TAL3044 &...cited2 TAL3228 & TAL3229 TGGTAGACCGCATGATGGcaatgaaccatggtcgTTTCCCAGATGCTGTGAA cited4a TAL3046 & TAL3047...TAL3047 TGAACCATGGTTCTGCTGgcggaggcctgcatggTTATCGCATGGGAATGAA cited4b TAL3048 & TAL3049 TGAACCACGGCCAGGGAAacgggggtctgcatggCTACCGGATGGGCATGAA...Factor H TAL3230 & TAL3231 TGATTACATGTGAACCTCcatcacgtgaccagcatgTGTCCACCTCCTTTTGGA cln3 TAL3054 & TAL3055 ...TCCCGTGCAAGGAATGAAtgcaaaaacatttcctTTCCCTTGTTTTGACGGA dcps TAL3416 & TAL3417 TGAACAGTCCACCGATAAcccagtggtagacttcCAGCTCACCCGCATTCTA ddx26b TAL3418 & TAL3419...TCAGAACTGCTCAAGTCCatttggtacgctttcaCAGCTCTGGATGTGGAGA DGKE TAL3238 & TAL3239 TATGGTGTCAAACCACAGtgcatgatgactgtctTTCCAGTCTGACCGATGA Dlk1 (site #1) TAL3240 &...

  4. Validated gRNA Sequences

    Type
    Collection
    ...Species PubMed ID Depositor OCT4 H. sapiens CTCCCATGCATTCAAACTG 66989 cut S. pyogenes 26028531 Huangfu OCT4...25337876 Ventura Amplicon, JAK2 H. sapiens GAGGCATATTCTTCTCCTGG 70660 cut S. pyogenes 26472758 Sabatini ... S. pyogenes 23849981 Qi CDH1 H. sapiens TGACTTGCGAGGGACGCATT 58781 cut S. pyogenes 24954249 Yamamoto ...23849981 Qi CYC1m promoter S. cerevisiae ACAGAGCACATGCATGCCAT 64385 activate S. pyogenes 23977949 Lu CYC1m...pyogenes 23287722 Church DNMT3A H. sapiens GCATGATGCGCGGCCCAAGG 41821 cut S. pyogenes 23287722 Church DNMT3B...pyogenes 24336571 Zhang EGFP A. victoria GGTGAACCGCATCGAGCTGA 51765 cut S. pyogenes 24336571 Zhang EGFP...pyogenes 23287722 Church GFP A. victoria GTGAACCGCATCGAGCTGAA 41819 cut S. pyogenes 23287722 Church GLuc...

  5. Zinc Finger Consortium: Zinc Finger Arrays

    Type
    Collection
    ...OPEN gAGCATCACCtcgctGGAGGTGAGc PTRF/Cavin1 OZ513 and OZ514 OPEN OPEN gACAGCCGTCtgcatctGGAGGTGCCc pitrm1...CODA OPEN cAACCTCAGCATAAAGCTGGAGGAg psen2 OZ577 and OZ578 CODA OPEN cGGCATCTCCAGAGCCCGAGGCGGACc zgc:162148...hydroxylase (dbh) OZ525 and OZ526 OPEN OPEN tCCCATCCTAtcgttGCATGAGCTc mdka (midkine-related growth factor) OZ527...lipoprotein receptor) OZ539 and OZ540 OPEN OPEN tTGCATCCACggcagTCGGCAGTGc Encephalopsin (Opn3) OZ541 and OZ542 OPEN...tTACTTCTGCaatgatGTGGTGGCTc vipr1 OZ559 and OZ560 OPEN OPEN gGCCATCAGCtcgcattGGAGAAGTAg NRF2b (nfe212b) OZ561 and OZ562 OPEN ...tTGCTGCCGCGGATGCTGTGGCGGTTa cx52.9 OZ583 and OZ584 OPEN OPEN cTTCATCTTCCGCATGCTGGTGCTg flt1 (A4QNT7_DANRE) OZ585 and OZ586 CODA...

  6. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...Forward Primer will use the sequence 5'-ATGTGGCATATCTCGAAGTAC-3' for the region that binds the ORF and...primer, making our Forward Primer 5'-GAATTCATGTGGCATATCTCGAAGTAC-3'. Many restriction enzymes do not cut...final Forward Primer sequence of 5'-TAAGCAGAATTCATGTGGCATATCTCGAAGTAC-3'. For the Reverse Primer, the design...of the ORF, including the stop codon (5'-TGGCATATCTCGAAGTACTGA-3'), then adding NotI (GCGGCCGC) and then.... This gives us a sequence of 5'-TGGCATATCTCGAAGTACTGAGCGGCCGCTAAGCA-3' (30bp with 18bp of homology to...chose for our reverse primer (5’-TGGCATATCTCGAAGTACTGAGCGGCCGCTAAGCA-3’) into this calculator we get a...

  7. Sequencing Primers

    Type
    Guide
    ... forward primer Full Primer List 3'AOX1 GCAAATGGCATTCTGACATCC (Invitrogen) For Pichia vectors with AOX1...5C promoter, forward primer Alpha-factor TACTATTGCCAGCATTGCTGC (Invitrogen) Alpha factor signal sequence...immediate early promoter, forward primer CRE-R GCAAACGGACAGAAGCATTT 5' end of Cre recombinase, reverse primer...cerevisiae GPD promoter, forward primer GW-3' GCATGATGACCACCGATATG 3' end of Gateway cassette, forward primer...MCS in L4440 vector, forward primer LacI-R GGCATACTCTGCGACATCGT 5' end of LacI, reverse primer LacZ-R GACAGTATCGGCCTCAGGAA...stem cell virus, forward primer MSCV-rev CAGCGGGGCTGCTAAAGCGCATGC Murine stem cell virus, reverse primer... Mouse U6 promoter, forward primer Myc GCATCAATGCAGAAGCTGATCTCA (BD Biosciences) Myc tag, forward primer...

  8. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ... 5’ CCGG AATGCCTACGTTAAGCTATAC CTCGAG GTATAGCTTAACGTAGGCATT TTTTTG 3’ Reverse oligo: 5’...AATTCAAAAA AATGCCTACGTTAAGCTATAC CTCGAG GTATAGCTTAACGTAGGCATT 3’ Back to Top C. Cloning Oligos into pLKO...
Showing: 1 - 8 of 8 results