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Tol2-LyzC-TagRFP-T-EEA1

Plasmid

#254776

Purpose

Neutrophil specific Early Endosome Antigen 1 expression in tol2 backbone

Depositor

Qing Deng

Article

Kim et al J Leukoc Biol. 2026 Feb 9;118(2):qiaf174

Insert

EEA1 (EEA1 Human)

Use

Zebrafish expression

Tags

TagRFP-T

Promoter

Lyzc

Available Since

May 4, 2026

Availability

Academic Institutions and Nonprofits only

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AAVS1 locus donor plasmid

Plasmid

#252870

Purpose

Plasmid encoding FRT recombination site and TetOn3G transactivator flanked by AAVS1 homology arms

Depositor

Norman Davey

Insert

TetOn3G transactivator and FRT recombination site

Expression

Mammalian

Available Since

March 24, 2026

Availability

Academic Institutions and Nonprofits only

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pYO979

Plasmid

#235756

Purpose

Protein expression of ScVPS38, untagged + ScVPS30 FRK to DDD at 430-432, untagged

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Inserts

VPS38 (VPS38 Budding Yeast)
VPS30 (VPS30 Budding Yeast)

Expression

Yeast

Mutation

FRK430-432DDD

Available Since

July 7, 2025

Availability

Academic Institutions and Nonprofits only

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pYO539

Plasmid

#235752

Purpose

Protein expression of ScVPS30 and ScVPS38

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Inserts

VPS30 (VPS30 Budding Yeast)
VPS38 (VPS38 Budding Yeast)

Expression

Yeast

Promoter

GAL-TDH3

Available Since

July 7, 2025

Availability

Academic Institutions and Nonprofits only

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MAC_C_CSF1R

Plasmid

#187767

Purpose

MAC-tagged gene expression of human CSF1R

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

CSF1R (CSF1R Human)

Expression

Mammalian

Available Since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pGST2-yVps23-UEV_C133A

Plasmid

#45924

Depositor

James Hurley

Article

Ren et al EMBO J. 2011 Jun 1;30(11):2130-9. doi: 1

Insert

yeast Vps23-UEV(1-160) (STP22 Budding Yeast)

Tags

GST

Expression

Bacterial

Mutation

C133A

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
BPK1520-sgRNA GLB1

Plasmid

#184378

Purpose

Expresses a sgRNA to edit GLB1 gene NM_000404.2_c.907A>G

Depositor

David Gilot

Article

Leclerc et al CRISPR J. 2023 Feb;6(1):17-31. doi: 10.1

Insert

sgRNA targeting GLB1 (GLB1 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pBoBi-hSEC61B-C-PEN2

Plasmid

#183648

Purpose

Lentiviral expression of a ER-residential PEN2.

Depositor

Sheng-cai Lin

Article

Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

Insert

PEN2 and SEC61B (PSENEN, SEC61B Human)

Use

Lentiviral

Tags

HA

Mutation

WT

Promoter

CMV

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pBoBi-hPEN2 2A

Plasmid

#183640

Purpose

Lentiviral expression of a PEN2 mutant losing its binding affinity to metformin at the N-terminus (cytosolic face).

Depositor

Sheng-cai Lin

Article

Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

Insert

PEN2 (PSENEN Human)

Use

Lentiviral

Tags

HA

Mutation

residues 35 and 40 of PEN2 mutated to alanine

Promoter

CMV

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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Negative feedback (NF) plasmid

Plasmid

#169747

Purpose

Standalone NF circuit plasmid that has the same implementation of the NF subcircuit in Equalizers.

Depositor

Francois St-Pierre

Article

Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

Insert

tetR-P2A-eGFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV-tetO2

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-Akt2(K14A/R25E)-Halo-PS

Plasmid

#86632

Purpose

Expresses eGFP-Akt2-HaloTag-PS fusion (cytosolic tandem)

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt2(K14A/R25E)-Halo-PS (AKT2 )

Expression

Mammalian

Available Since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

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pOGGFPR

Plasmid

#64398

Purpose

Oat globulin Glo1 promoter driving expression of GFP

Depositor

Claudia Vickers

Article

Vickers et al Plant Mol Biol. 2006 Sep;62(1-2):195-214

Insert

Oat globulin Glo1 promoter

Tags

GFP

Expression

Plant

Promoter

Oat globulin Glo1

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only
pT.cruzi-polyubiquitin_6xHis

Plasmid

#256080

Purpose

Production of a T. cruzi antigen, which can be used in serological assays

Depositor

Rick Tarleton

Article

Bustamante et al PLoS Negl Trop Dis. 2022 Oct 31;16(10):e

Insert

Polyubiquitin

Tags

6xHis-STag

Expression

Bacterial

Promoter

T7lac

Available Since

May 19, 2026

Availability

Academic Institutions and Nonprofits only

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Tol2-Lyzc-mCherry-Rab4 DN

Plasmid

#254774

Purpose

Neutrophil specific Dominant Negative Rab4 in tol2 backbone

Depositor

Qing Deng

Article

Kim et al J Leukoc Biol. 2026 Feb 9;118(2):qiaf174

Insert

Rab4 (RAB4A Human)

Use

Zebrafish expression

Mutation

DN(S27N)

Promoter

Lyzc

Available Since

May 4, 2026

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-FL Rabep1-T2A-RFP-Caax

Plasmid

#254770

Purpose

Neutrophil specific Full length Rabep1 in tol2 backbone

Depositor

Qing Deng

Article

Kim et al J Leukoc Biol. 2026 Feb 9;118(2):qiaf174

Insert

Rabep1 (RABEP1 Human)

Use

Zebrafish expression

Promoter

Lyzc

Available Since

May 4, 2026

Availability

Academic Institutions and Nonprofits only

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WoFabI-pNIC-Bsa4

Plasmid

#240437

Purpose

Expresses enoyl-ACP reductase FabI [Wolbachia endosymbiont of Brugia malayi] under T7 with HIS tag in bacterial cells)

Depositor

Josh Beckham

Insert

Enoyl-acyl carrier protein reductase

Tags

6x His-tag

Expression

Bacterial

Promoter

T7

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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Dendra-Rab11a

Plasmid

#184038

Purpose

Expresses red to green photoconvertible Rab11a

Depositor

Heidi Hehnly

Article

Krishnan et al Life Sci Alliance. 2022 Mar 18;5(7). pii

Insert

Rab11 (RAB11A Human)

Tags

Dendra

Expression

Mammalian

Promoter

CMV

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pMJA105

Plasmid

#128521

Purpose

Expresses amyloid human Lysozyme in Pichia pastoris

Depositor

Marcos Alcocer

Article

Johnson et al J Mol Biol. 2005 Sep 30;352(4):823-36. d

Insert

Artificial gene for human lysozyme T70N (LYZ Human)

Expression

Yeast

Promoter

AOX1

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pET12a-AL-103 delProIns

Plasmid

#47095

Depositor

Marina Ramirez-Alvarado

Insert

AL-103 delProIns (IGKV3-20 Human)

Tags

ompT

Expression

Bacterial

Mutation

delete aa95

Promoter

T7

Available Since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only
pT.cruzi-microtubuleAP_6xHis

Plasmid

#256081

Purpose

Production of a T. cruzi antigen, which can be used in serological assays

Depositor

Rick Tarleton

Article

Bustamante et al PLoS Negl Trop Dis. 2022 Oct 31;16(10):e

Insert

Microtubule-associated protein

Tags

6xHis-STag

Expression

Bacterial

Promoter

T7lac

Available Since

May 18, 2026

Availability

Academic Institutions and Nonprofits only

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