We narrowed to 18,167 results for: STO;

1. pENTR221 UBR5

   Plasmid

   #81062

   Purpose

   Gateway entry vector encoding full length UBR5

   Depositor

   Darren Saunders

   Article

   Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

   Insert

   Ubiquitin protein ligase E3 component N-recognin 5 (UBR5) (UBR5 Human)
   

   Use

   Cloning

   Mutation

   K503R

   Promoter

   N/A

   Available Since

   Jan. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEJS2601_AAV-Nme2-ABE8e-i1-U6-2xSapI

   Plasmid

   #201683

   Purpose

   All-in-one AAV plasmid expressing Nme2-ABE8e-i1 and U6 sgRNA cassette. SapI enables cloning new spacers.

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

   Insert

   Nme2-ABE8e-i1
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Promoter

   U1a

   Available Since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLKO.1-puro_CMV_NES-Caprola_on-mEGFP

   Plasmid

   #194694

   Purpose

   CMV driven expression of the consitutively active calcium recorder positive control Caprola_on fused to mEGFP for neuronal expression through lentivirus transduction

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_on-mEGFP
   

   Use

   Lentiviral

   Tags

   mEGFP

   Promoter

   CMV

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-pTH-iCre-WPREpA

   Plasmid

   #213130

   Purpose

   Truncated rat TH promoter expressing iCre

   Depositor

   Ulrik Gether

   Article

   Lycas et al Cell Rep. 2022 Sep 27;40(13):111431. doi

   Insert

   iCre
   

   Use

   AAV and Mouse Targeting

   Tags

   None

   Expression

   Mammalian

   Promoter

   Truncated TH promoter from rat (Rattus norvegicus…

   Available Since

   Feb. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Jag1-HA pIRES

   Plasmid

   #17336

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

   Insert

   Jagged1 (Jag1 Rat)
   

   Tags

   HA X3

   Expression

   Mammalian

   Mutation

   A silent C->G mutation at 3237

   Available Since

   April 24, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. 5_T7-GAP43-mScarlet3

   Plasmid

   #225932

   Purpose

   In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the GAP43 membrane localisation signal

   Depositor

   Michalis Averof

   Article

   Karapidaki et al bioRxiv 2024.11.12.623055

   Insert

   GAP43-mScarlet3
   

   Use

   In vitro transcription

   Promoter

   T7

   Available Since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX-5X-1-LARP7

   Plasmid

   #113545

   Purpose

   Produces an N-terminal GST-tagged full length LARP7 protein.

   Depositor

   Blerta Xhemalce

   Article

   Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

   Insert

   LARP7 (LARP7 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGL4.26-Ccnd2PromoterRegion2(0.5kb)

   Plasmid

   #228059

   Purpose

   0.5 kb from mouse Ccnd2 promoter in front of firefly luciferase reporter

   Depositor

   Kenneth Chen

   Article

   Fraire et al bioRxiv [Preprint]. 2024 Apr 30:2024.04.

   Insert

   Ccnd2 promoter (Ccnd2 Mouse)
   

   Use

   Luciferase

   Available Since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL4.26-Igf2Promoter

   Plasmid

   #228060

   Purpose

   0.9 kb from mouse Igf2 promoter in front of firefly luciferase reporter

   Depositor

   Kenneth Chen

   Article

   Fraire et al bioRxiv [Preprint]. 2024 Apr 30:2024.04.

   Insert

   Igf2 promoter (Igf2 Mouse)
   

   Use

   Luciferase

   Available Since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti CMV SNAP MDC1

    Plasmid

    #227375

    Purpose

    SNAP–MDC1 expression

    Depositor

    David Pellman

    Article

    Papathanasiou et al Nature. 2023 Jul;619(7968):184-192. doi:

    Insert

    MDC1, mediator of DNA damage checkpoint 1 (MDC1 Human)
    

    Use

    Lentiviral

    Tags

    SNAP

    Expression

    Mammalian

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDule-3-nitroTyrosine (5B)

    Plasmid

    #85498

    Purpose

    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.

    Depositor

    Ryan Mehl

    Article

    Cooley et al Biochemistry. 2014 Apr 1;53(12):1916-24.

    Insert

    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    

    Tags

    None

    Expression

    Bacterial

    Mutation

    Y32H H70C D158S I159A L162R

    Promoter

    lpp (constitutive)

    Available Since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-UBC-ERT2-iCre-ERT2

    Plasmid

    #178060

    Purpose

    Conditionally active form of Cre recombinase (activated in response to tamoxifen) under UBC promoter.

    Depositor

    Edward Boyden

    Article

    Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    ERT2-iCre-ERT2
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Human ubiquitin C (UBC) promoter

    Available Since

    Oct. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHS0810 pcDNA3.1(+) CMV-SV40 NLS-OgeuIscB-nucleoplasmin NLS-3xHA

    Plasmid

    #176540

    Purpose

    Mammalian expression of nuclear localized OgeuIscB

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

    Insert

    OgeuIscB
    

    Tags

    Nucleoplasmin NLS-3xHA and SV40 NLS

    Expression

    Mammalian

    Available Since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNLF1-C_hCOL4A5-Nluc

    Plasmid

    #198113

    Purpose

    Express hCOL4A5 in mammalian cells

    Depositor

    Jeffrey Miner

    Article

    Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

    Insert

    COL4A5 (COL4A5 Human)
    

    Tags

    Nluc

    Expression

    Mammalian

    Promoter

    CMV, beta-globin/IgG chimeric intron

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. FLAG-FOXO3a TM

    Plasmid

    #8361

    Depositor

    Michael Greenberg

    Article

    Brunet et al Science 2004 Mar 26;303(5666):2011-5. Ep

    Insert

    FOXO3a (FOXO3 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    T32A, S253A, S315A

    Available Since

    Feb. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pT2/sh-Col1a1/GFP4_Seq1.2

    Plasmid

    #201403

    Purpose

    Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

    Depositor

    Maria Castro

    Article

    Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

    Insert

    COL1A1
    

    Expression

    Mammalian

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-flag-YTHDF2-N

    Plasmid

    #52302

    Purpose

    Flag-tagged YTHDF2 N-terminal domain, made by mutating to E384(GAA) to stop codon(TAA)

    Depositor

    Chuan He

    Article

    Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

    Insert

    YTHDF2 (YTHDF2 Human)
    

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Changed E384 to stop codon

    Promoter

    T7

    Available Since

    April 14, 2014

    Availability

    Academic Institutions and Nonprofits only

18. AAV-actin prom-dsRed-scramble shRNA

    Plasmid

    #67879

    Purpose

    AAV vector to express dsRed sh RNA (scramble)

    Depositor

    William Wisden

    Article

    Zhang et al Nat Neurosci. 2015 Apr;18(4):553-61. doi

    Insert

    actin prom-dsRed-scramble shRNA
    

    Use

    AAV and RNAi

    Expression

    Mammalian

    Promoter

    chicken beta actin

    Available Since

    Aug. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pAS_4xMmaPylT(UUA)_EF1_MmaPylRS

    Plasmid

    #174896

    Purpose

    Mma PylRS expression for ochre suppression in mammalian cells; transient or piggy bac mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    Mma PylRS (pylS Methanosarcina mazei)
    

    Expression

    Mammalian

    Promoter

    EF1

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pmScarlet-H_Giantin_C1

    Plasmid

    #85049

    Purpose

    In vivo visualization of the Golgi apparatus (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    Giantin (GOLGB1 Human)
    

    Expression

    Mammalian

    Mutation

    aa 3131-3259

    Promoter

    CMV

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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