We narrowed to 17,095 results for: Por

1. pCOTS-pyl-GFP(35TAG)

   Plasmid

   #92047

   Purpose

   This is a S. elongatus (PCC7942) cyanobacterial plasmid that encodes the pylRS orthogonal translation system. In addition it encodes for a GFP reporter with TAG mutation at site 35.

   Depositor

   Lital Alfonta

   Article

   Chemla et al Biochemistry. 2017 Apr 12. doi: 10.1021/

   Inserts

   EGFP
   Pyrrolysyl tRNA(cua)
   Pyrrolysyl tRNA synthetase (methanosarcina mazei)
   

   Use

   Replicative expression plasmid for cyanobacteria …

   Tags

   Histag

   Expression

   Mutation

   Tyrosine in position 35 of the GFP was mutated f…

   Promoter

   LeuP (native S. elongatus promoter), PpsbII, and …

   Available Since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-mCherry-hCdt1(1-100)ΔCy

   Plasmid

   #193759

   Purpose

   Expresses N-CRL4Cdt2 reporter(mCherry-hCdt1(1-100)ΔCy)

   Depositor

   Tobias Meyer

   Article

   Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

   Insert

   mCherry-hCdt1(1-100)ΔCy (CDT1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   human Cdt1 amino acids 1-100 w/ ΔCy mutation (aa6…

   Promoter

   EF1a

   Available Since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR221_SLC35F2

   Plasmid

   #132121

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC35F2 (SLC35F2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 25, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pUC19 EGFRup-CD3ε-mNeonGreen-EGFRdown

   Plasmid

   #188634

   Purpose

   HDR template for CD3ε-mNeonGreen knock-in at C-terminus of human EGFR

   Depositor

   Jared Toettcher

   Article

   Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

   Insert

   EGFR (EGFR Human, Synthetic)
   

   Use

   CRISPR

   Tags

   CD3ε-mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAVS1-TetOn-3XFLAG-renilla-beta-globin-control-AAVS1

   Plasmid

   #184396

   Purpose

   Donor plasmid for stable integration of Renilla luciferase control reporter at AAVS1

   Depositor

   Robert Bradley

   Article

   Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

   Insert

   Renilla luciferase beta-globin
   

   Use

   CRISPR; Donor plasmid for hdr

   Tags

   3X FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Tet-On 3G

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLex 983-Micu2

   Plasmid

   #50372

   Purpose

   Expression in mammalian cells

   Depositor

   Vamsi Mootha

   Article

   Plovanich et al PLoS One. 2013;8(2):e55785. doi: 10.1371

   Insert

   MICU2 (MICU2 Human)
   

   Use

   Lentivirus

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

7. p323.hSyn.Halo-mFMRP.Wpre

   Plasmid

   #185909

   Purpose

   Expresses FMRP fused to HaloTag for protein localization through fluorescence imaging

   Depositor

   Young J Yoon

   Article

   Monday et al Neuron. 2022 Aug 17;110(16):2588-2606.e6

   Insert

   HaloTag fused to mouse Fragile X mental retardation protein (Ctsa Mouse)
   

   Use

   Lentiviral

   Tags

   HaloTag

   Expression

   Mutation

   Promoter

   hSyn (human Synapsin promoter)

   Available Since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pXL003-ishRNA-beta-catenin-2

   Plasmid

   #36299

   Depositor

   Sean Palecek

   Article

   Lian et al Proc Natl Acad Sci U S A. 2012 May 29.

   Insert

   shRNA of beta-catenin-2 (CTNNB1 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   IRES-RFP-Puro

   Expression

   Mammalian

   Mutation

   Promoter

   TetO-H1

   Available Since

   Aug. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pJOP-HTT-HR18Q

   Plasmid

   #87228

   Purpose

   HTT HR arms with 18 CAG repeats and piggyBac selection cassette

   Depositor

   Mahmoud Pouladi

   Article

   Xu et al Stem Cell Reports. 2017 Feb 21. pii: S22

   Insert

   1.7kb HTT 5' homology arm, 2.5kb HTT 3' homology arm
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a driving selection cassette

   Available Since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMRXIP-GFP-OSBP(PHD)

    Plasmid

    #221745

    Purpose

    Stable expression of GFP-OSBP(PHD) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

    Insert

    OSBP (OSBP Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    amino acids 87–185

    Promoter

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCb.CAG-HaloTag-GA-ZF-KrabA-KIF5A 378-1027

    Plasmid

    #134611

    Purpose

    Mammalian expression of mouse KIF5A 378-1027

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KIF5A (Kif5a Mouse)
    

    Use

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    WT with deletion of the 377 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available Since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pB1H2 UV5 Zif268-5ala (2-hybrid bait plasmid)

    Plasmid

    #128165

    Purpose

    UV5 promoter drives expression of Zif268-5alanine peptide fusion. Pair with pGHUC w-ERBIN as a negative control (doesn't turn on HIS3/GFP).

    Depositor

    Marcus Noyes

    Article

    Ichikawa et al ACS Synth Biol. 2019 May 17;8(5):918-928

    Insert

    10 amino acid glycine/serine linker + 5alanines
    

    Use

    Synthetic Biology

    Tags

    FLAG and zif268

    Expression

    Bacterial

    Mutation

    Promoter

    UV5

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX VAMP7 (1-188)

    Plasmid

    #42315

    Depositor

    Thierry Galli

    Article

    Martinez-Arca et al J Cell Biol. 2000 May 15;149(4):889-900.

    Insert

    VAMP7 Cytoplasmic domain (1-181) (VAMP7 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Δ181-220

    Promoter

    Ptac

    Available Since

    March 12, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pLJM60-FLAG-SLC38A9.1 (delta110)

    Plasmid

    #71861

    Purpose

    lentiviral stable expression

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Jan 9;347(6218):188-94. do

    Insert

    SLC38A9.1 (SLC38A9 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    codon optimized, missing first 110 amino acids

    Promoter

    CMV

    Available Since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pDONR221_SLC35D3

    Plasmid

    #132110

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC35D3 (SLC35D3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 21, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pDONR221_SLC19A3

    Plasmid

    #132045

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC19A3 (SLC19A3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pCDF-Az

    Plasmid

    #197101

    Purpose

    To express the azidophenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into azidophenylalanine

    Depositor

    Kensaku Sakamoto

    Article

    Yamaguchi et al Bioconjug Chem. 2016 Jan 20;27(1):198-20

    Insert

    TyrRS variant, amber suppressor tRNA, kanamycin resistance gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Thr32, Asn107, Pro158, Leu159, Gln162, Arg286 (Ty…

    Promoter

    Available Since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-hSyn-miRFP-6xFLAG

    Plasmid

    #161614

    Purpose

    Cell morphology marker construct. Contains a near infrared fluorescent protein miRFP and six FLAG tags.

    Depositor

    Edward Boyden

    Article

    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

    Insert

    miRFP-6xFLAG
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Human synapsin 1 promoter

    Available Since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. TetO-FUW-Hes1

    Plasmid

    #61534

    Purpose

    Expresses Hes1 in mammalian cells

    Depositor

    Rudolf Jaenisch

    Article

    Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

    Insert

    Hes1 (Hes1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-hSyn-mRuby3-6xFLAG

    Plasmid

    #161613

    Purpose

    Cell morphology marker construct. Contains a red fluorescent protein mRuby3 and six FLAG tags.

    Depositor

    Edward Boyden

    Article

    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

    Insert

    mRuby3-6xFLAG
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Human synapsin 1 promoter

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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