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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 18,614 results for: SHE

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Showing: 8221 - 8240 of 18614 results

  1. CUP1-∆ssCPY*-mGFP

    Plasmid
    #212197
    Purpose
    Fluorescent reporter for cytoplasmic aggregates
    Depositor
    Mark Leake, Chris MacDonald
    Insert
    Carboxypeptidase Y variant (PRC1 Budding Yeast)
    Tags
    mEGFP (monomeric version EGFP, A206K mutation)
    Expression
    Yeast
    Mutation
    Deletion signal peptide, G255A mutation
    Promoter
    CUP1
    Available Since
    Nov. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    212197-plasmid-map-sequence-id-450955

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  2. pIND4-RC-C2-M210TAG+M252V-oCNF

    Plasmid
    #222995
    Purpose
    Expresses 3 subunits of photosynthetic reaction center from R. sphaeroides and aaRS-tRNA pair for ortho-phenylalanine variants
    Depositor
    Steven Boxer
    Inserts
    puhA
    pufL
    pufM
    Phe variants PylRs
    Mm Pyl tRNA for oF incorporation
    Tags
    6xHis
    Mutation
    changed Tyr M210 to amber stop codon (TAG) for am…
    Promoter
    rrnB
    Available Since
    Sept. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    222995-plasmid-map-sequence-id-444743

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  3. 8TBX

    Plasmid
    #218228
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    DDX1 (DDX1 Human)
    Use
    Baculovirus expression
    Tags
    6xHis-TEV
    Promoter
    Polyhedrin
    Available Since
    April 17, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    218228-plasmid-map-sequence-id-430075

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  4. pET50_G495R∆2CARD

    Plasmid
    #216805
    Purpose
    Expresses a single point mutant of melanoma differentiation-associated protein 5 lacking its 2 CARD domains
    Depositor
    Sun Hur
    Insert
    melanoma differentiation-associated protein 5 delta 2 CARD domains (IFIH1 Human)
    Expression
    Bacterial
    Mutation
    G495R/∆1-287
    Available Since
    April 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    216805-plasmid-map-sequence-id-428836

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  5. 8GCY

    Plasmid
    #214440
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CBLB:PBC020-G02:C248501 (CBLB Human)
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 11, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    214440-plasmid-map-sequence-id-424893

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  6. 8DYS

    Plasmid
    #214439
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    EIF2A:PBC022-C06:C245159 (EIF2A Human)
    Use
    Baculovirus
    Tags
    6xHis-TEV
    Promoter
    Polyhedrin
    Available Since
    Feb. 28, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    214439-plasmid-map-sequence-id-424355

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  7. pET28-mEGFP(A206K)-TwStrep

    Plasmid
    #208622
    Purpose
    for E. coli expression of C-ter Twin Strep tagged mEGFP(A206K), EGFP cloned from pEGFP-C1, A206K introduced by seamless (Gibson) clone
    Depositor
    Quanfu Ma
    Insert
    mEGFP(A206K)
    Tags
    6xHis and Twin Strep
    Expression
    Bacterial
    Mutation
    (compared with pEGFP-C1, protein level) A206K
    Promoter
    T7
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208622-plasmid-map-sequence-id-415916

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  8. pGB-05-06-GST-ATG14

    Plasmid
    #187987
    Purpose
    Plasmid for the protein expression of GST-ATG14 in a bacterial and insect expression system. Internal_Reference: SMC1321
    Depositor
    Sascha Martens
    Insert
    ATG14 (ATG14 Human)
    Tags
    GST
    Expression
    Bacterial and Insect
    Available Since
    Feb. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    187987-plasmid-map-sequence-id-374440

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  12. Ef1a-DIO-somAion-citrine

    Plasmid
    #192573
    Purpose
    AAV-vector for optogenetic long-term silencing of Cre-positive neurons
    Depositor
    Simon Wiegert
    Insert
    somAion
    Use
    AAV
    Tags
    Citrine - soma-targeting motif from Kv2.1 channel
    Expression
    Mammalian
    Mutation
    T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…
    Promoter
    Ef1-alpha
    Available Since
    Dec. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    192573-plasmid-map-sequence-id-379368

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  13. pB6-Hipp11-td-sfGFP-cre reporter-3pA stop-FNF-DTA

    Plasmid
    #183026
    Purpose
    B6J Hipp11-FNF-pCAG-loxP-3xSV40pA-loxP-td-sfGFP-WPRE-bpA allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    td-sfGFP
    Use
    Cre/Lox and Mouse Targeting
    Expression
    Bacterial and Mammalian
    Available Since
    April 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    183026-plasmid-map-sequence-id-360495

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  14. pB6-Hipp11-tdTomato-cre reporter-3pA stop-FNF-DTA

    Plasmid
    #183025
    Purpose
    B6J Hipp11-FNF-pCAG-loxP-3xSV40pA-loxP-tdTomato-WPRE-bpA allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    tdTomato
    Use
    Cre/Lox and Mouse Targeting
    Expression
    Bacterial and Mammalian
    Available Since
    April 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    183025-plasmid-map-sequence-id-360497

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  15. pB6-Hipp11-tdTomato cre reporter-FNF-DTA

    Plasmid
    #183024
    Purpose
    B6J Hipp11-pCAG-loxP-FNF-bGHpA-3xSV40pA-loxP-tdTomato-WPRE-bpA allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    tdTomato
    Use
    Cre/Lox and Mouse Targeting
    Expression
    Bacterial and Mammalian
    Available Since
    April 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    183024-plasmid-map-sequence-id-360499

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  16. MDKA-c014

    Plasmid
    #175464
    Purpose
    Protein expression/secretion in mammalian cells. Entire CDS with ectopic signal peptide, V21-D143. Fused to IgG Fc.
    Depositor
    Opher Gileadi
    Insert
    MDK (MDK Human)
    Tags
    HRV 3C-Fc-His6 and PTPRS (Gg) SP
    Expression
    Mammalian
    Available Since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175464-plasmid-map-sequence-id-353255

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  17. EPB41L3A-c021

    Plasmid
    #175460
    Purpose
    Protein expression in bacterial cells. FERM domain, P107-L390. Used for crystallography (PDB:6IBE).
    Depositor
    Opher Gileadi
    Insert
    EPB41L3 (EPB41L3 Human)
    Tags
    His6-TEV
    Expression
    Bacterial
    Available Since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175460-plasmid-map-sequence-id-353154

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  20. pDK362

    Plasmid
    #134194
    Purpose
    Retroviral plasmid with SKAP insert
    Depositor
    Iain Cheeseman
    Insert
    SKAP 166-316-LAP with G5 and Jens siRNA hardening (KNSTRN Human)
    Use
    Retroviral
    Tags
    LAP (GFP)
    Expression
    Mammalian
    Mutation
    aa 166-316 with G5 and Jens siRNA hardening
    Available Since
    Feb. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    134194-plasmid-map-sequence-id-272444

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Showing: 8221 - 8240 of 18614 results