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Plasmid#182643PurposeMammalian expression vector encoding a protein fusion composed of an N-terminal ER targeting sequence of hemagglutinin (HA), followed by mCherry and murine CDMPR (without its signal peptide)DepositorInsertCDMPR
UseRetroviralTagsN-terminal ER targeting sequence of hemagglutinin…ExpressionMammalianPromoterCMVAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA/golgin-160/myc
Plasmid#180632PurposeExpression of WT golgin-160DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgolgin-160 (GOLGA3 Human)
TagsMyc-HisExpressionMammalianAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-CDK13_IDG-K
Plasmid#135266PurposeGateway destination clone of CDK13 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-CDK13 (CDK13 Human)
UseLentiviral; Gateway destinationTagsV5-miniTurboExpressionMammalianPromoterUbiquitinAvailable sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
AC27 pEGFPN1 anti-AMIGO-1 nanobody
Plasmid#135226PurposeMammalian expression of anti-AMIGO-1 (Mouse) recombinant llama nanobody for use as an intrabodyDepositorInsertanti-AMIGO-1 (Mouse) recombinant llama nanobody AC27 (Amigo1 L. glama (llama))
Tags6xHis-HA-EGFPExpressionMammalianAvailable sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIMVAd40R
Plasmid#98309PurposeEnhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of GAL promoters; integrated into ERG9 locus;DepositorInsertERG9C-terminal-TURA3- PGAL7>MVD1>TPRM9-PGAL2>-ERG12>TNATTIDP1ExpressionYeastAvailable sinceFeb. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMSXND1-hPPT1
Plasmid#85817PurposeExpression of hPPT in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman PPT1 (PPT1 Human)
ExpressionMammalianAvailable sinceJan. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
Sema4c-Fc-His
Plasmid#72156PurposeExpresses the extracellular region of the Sema4C protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pESC-URA-hPOLISc
Plasmid#60819Purposeto express human Pol iota recoded to yeast codons in yeastDepositorInsertPOLI (POLI Human)
Tagsc-mycExpressionYeastMutationoptimized to yeast codon usagePromoterGAL1Available sinceMarch 9, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYIAL31
Plasmid#60807Purposeused to make mutations in yeast POL1DepositorPublicationAvailable sinceFeb. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
PhyB-mCherry-SNF7
Plasmid#51574PurposeConstitutively express endosome localized PhyB in Saccharomyces cerevisiaeDepositorInsertPhyB (PHYB Mustard Weed)
TagsSNF7 and mCherryExpressionYeastMutation1-908aa onlyPromoterADH1prAvailable sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTK64_GFP-NuMA-Bonsai
Plasmid#46351DepositorInsertNuMA deleted 414-1549 (NUMA1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationdeleted amino acid 414-1549; L1589F mutation in N…PromoterpBABEAvailable sinceAug. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEMS1062
Plasmid#29011PurposeThe plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.DepositorInsertPle155-Cre/EGFP-NLS
UseHigh copy number homologous recombination plasmid…ExpressionMammalianPromoterMiniPromoter Ple155 from the human PCP2 geneAvailable sinceFeb. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDEST51-LRRK2-R1441H
Plasmid#25083DepositorPublicationInsertLRRK2 (LRRK2 Human)
UseGateway CloningTags6XHis and V5ExpressionMammalianMutationArg 1441 mutated to HisAvailable sinceJune 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMT440
Plasmid#8609DepositorPublicationInsertbarnase, barstar
Tagsas pMT416ExpressionBacterialMutationV36 codon of barnase of pMT416 replaced by polyli…Available sinceJan. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
DCT.VV.Orf42
Plasmid#12730PurposeThis plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertVV.Orf42
ExpressionMammalianAvailable sinceDec. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
ALIX-His(1-868, P801G)
Plasmid#186808PurposeCodon-optimized bacterial expression plasmid. Expresses ALIX-His(1-868, P801G).DepositorInsertALIX-His (1-868, P801G) (PDCD6IP Human)
TagsHis6ExpressionBacterialMutationProline 801 to GlycineAvailable sinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
MEK1C222
Plasmid#164638PurposeBacterial expression plasmid for His6-MEK1C222DepositorInsertmitogen-activated protein kinase kinase 1 (MAP2K1 Human)
TagsHis6-tagExpressionBacterialMutationG(-19)F/S222C/C277S/C376SPromoterT7Available sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
B108-Otx2-IRES-Cre
Plasmid#73993PurposeB108 enhancer of Blimp1 gene drives Otx2 (isoform b) and Cre expression.DepositorInsertB108-Otx2 (isoform b)-IRES-Cre (Otx2 Mouse)
ExpressionMammalianAvailable sinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
B108-GFP-IRES-AP
Plasmid#73988PurposeB108 enhancer of Blimp1 gene drives EGFP expression.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertB108 enhancer
ExpressionMammalianAvailable sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV5 KSR1 T274V
Plasmid#25975DepositorInsertKinase suppressor of Ras 1 (Ksr1 Mouse)
TagsFlagExpressionMammalianMutationchanged threonine 274 to valineAvailable sinceAug. 24, 2010AvailabilityAcademic Institutions and Nonprofits only