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We narrowed to 16,071 results for: jun

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Showing: 8301 - 8320 of 16071 results

  2. gCBEv2

    Plasmid
    #220617
    Purpose
    vector for encoding an engineered glycosylase-based cytosine (C) base editor (gCBEv2) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Publication
    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hUNG2-nSpCas9
    Expression
    Mammalian
    Mutation
    hUNG2(del1-88;N213D,K184A,A214V), SpCas9(D10A)
    Promoter
    hU6, EF1a
    Available since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220617-plasmid-map-sequence-id-436955

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  3. pαH-rS2d-HexaPro

    Plasmid
    #183515
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein rS2d-HexaPro with (682-685) furin side replaced with GSAS and mutation S383C, D985C, F817P, A892P, A899P, A942P, K986P,V987P
    Depositor
    Priyamvada Acharya
    Publication
    Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Spike (S-GSAS-rS2d.HexaPro) (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Available since
    May 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    183515-plasmid-map-sequence-id-362649

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  5. pCDH-EF1-E-'No'Mi-Red-T2A-Puro

    Plasmid
    #246642
    Purpose
    EF-1alpha driven EV reporter E-'No'Mi-R, a re-engineered tetraspanin scaffold tagged with bioluminescent and fluorescent reporter proteins. Nanoluc and Flag tag are removable with TEVp.
    Depositor
    Koen Breyne
    Publication
    Obuchi et al J Extracell Vesicles. 2025 Jun;14(6):e70
    Insert
    cleavable E-NoMi-Red
    Use
    Lentiviral
    Tags
    FLAG-Nanoluciferase and mCherry
    Expression
    Mammalian
    Promoter
    EF-1alpha
    Available since
    Oct. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    246642-plasmid-map-sequence-id-489517

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  6. PX458-3xHA-en31FnCas9ABEmax8.17d

    Plasmid
    #201956
    Purpose
    Mammalian expression plasmid of en31FnCas9ABEmax8.17d base editor with T2A-EGFP and cloning backbone for sgRNA
    Depositor
    Debojyoti Chakraborty
    Publication
    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    bpNLS-en31FnCas9ABEmax8.17d-bpNLS-3xHA-T2A-EGFP
    Use
    CRISPR
    Tags
    3xHA, NLS, and T2A-EGFP
    Expression
    Mammalian
    Promoter
    Cbh
    Available since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    201956-plasmid-map-sequence-id-405311

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  10. pCMV_EE-NG-nCas9

    Plasmid
    #163542
    Purpose
    Mammalian CG-to-GC base editing
    Depositor
    David Liu
    Publication
    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EE-NG-nCas9
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    nCas9 (D10A)EE (R126E, R132E)NG (L111R, D1135V, G…
    Available since
    Dec. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    163542-plasmid-map-sequence-id-320177

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  11. mE-GFP2A

    Plasmid
    #236247
    Purpose
    Middle entry fluorescent GFP reporter with a viral 2A peptide for use with the Gateway cloning system; allows 3' transgene to be transcribed on the same mRNA but translated separately
    Depositor
    James Amatruda, Genevieve Kendall
    Publication
    Kendall et al eLife. 2018 Jun 5;7. pii: 33800. doi: 10
    Insert
    eGFP
    Expression
    Mammalian
    Mutation
    Added a viral2A sequence with no stop codon
    Available since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    236247-plasmid-map-sequence-id-486040

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  13. pIL6-PMEL-hbb

    Plasmid
    #239868
    Purpose
    Template for in vitro transcription of PMEL (gp100), with signal peptide replaced by the IL6 signal peptide, flanked by the human haemoglobin beta 5' and 3' UTRs.
    Depositor
    Catherine Jopling
    Publication
    Smart et al Mol Ther Nucleic Acids. 2025 Jun 24;36(3
    Insert
    Full premelanosome coding sequence with IL6 signal peptide, flanked by human haemoglobin beta 5' and 3' UTRs (PMEL Human)
    Expression
    Mammalian
    Mutation
    Valine to Glycine change is at amino acid 26 of n…
    Promoter
    T7 (included as part of insert)
    Available since
    July 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    239868-plasmid-map-sequence-id-477826

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  14. pET30a-SSDH

    Plasmid
    #239573
    Purpose
    Expresses E. coli succinate semialdehyde dehydrogenase (SSDH/gabD) for recombinant protein purification. Contains a His6-tag on its C-terminus for Ni2+ affinity chromatography.
    Depositor
    William Plaxton
    Publication
    Benidickson et al Biol Methods Protoc. 2025 Jun 20;10(1):b
    Insert
    Succinate-semialdehyde dehydrogenase (gabD E. coli)
    Tags
    His6
    Expression
    Bacterial
    Available since
    June 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    239573-plasmid-map-sequence-id-476594

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  18. pET-30a (+)_CDK2-7 chimera (I10L,T14Q,V64I,K88E,K89V,D92K,Q131N)

    Plasmid
    #217014
    Purpose
    Expression of CDK2-7 chimera in E.coli
    Depositor
    Manjeet Mukherjee
    Publication
    Mukherjee et al Structure. 2024 Jun 9:S0969-2126(24)0018
    Insert
    CDK2-7 chimera (I10L,T14Q,V64I,K88E,K89V,D92K,Q131N) (CDK7, CDK2 Human)
    Tags
    C3 protease cleavable GST tag
    Expression
    Bacterial
    Mutation
    CDK2 (I10L,T14Q,V64I,K88E,K89V,D92K,Q131N)
    Available since
    July 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    217014-plasmid-map-sequence-id-431050

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  19. pREMI-ZA

    Plasmid
    #183736
    Purpose
    Plasmid for a restriction enzyme mediated integration (REMI) in Komagataella phaffii
    Depositor
    Jun Ishii
    Publication
    Ito et al Commun Biol. 2022 Jun 8;5(1):561. doi: 1
    Insert
    Sh ble
    Use
    Random insertional gene deletion in komagataella …
    Promoter
    TEF1 promoter
    Available since
    March 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    183736-plasmid-map-sequence-id-372719

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Showing: 8301 - 8320 of 16071 results