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Showing: 821 - 840 of 8082 results

  5. pF1-Hs Cdk1/cyclin B1/Cks1

    Plasmid
    #177329
    Purpose
    Co-expression of the human active Cdk1/cyclin B1/Cks1 kinase complex
    Depositor
    Andreas Boland
    Article
    Yu et al Nature. 2021 Aug;596(7870):138-142. doi:
    Inserts
    Cyclin-dependent kinase 1 (CDK1 Human)
    G2/mitotic-specific cyclin-B1 (CCNB1 Human)
    Cyclin-dependent kinases regulatory subunit 1 (CKS1B Human)
    Use
    Tags
    2 x Strep-tag and 8 x His
    Expression
    Insect
    Mutation
    Promoter
    p10 and polh
    Available since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    177329-plasmid-map-sequence-id-348397

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  7. pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP (AAV5)

    Viral Prep
    #35509-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP (#35509). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP plasmid DNA. EF1a-driven, Cre-dependent, humanized channelrhodopsin E123T/T159C mutant fused to EYFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Article
    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do
    Promoter
    EF1a
    Tags
    EYFP (Cre-dependent)
    Available since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only

  8. pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP (AAV9)

    Viral Prep
    #35509-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP (#35509). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP plasmid DNA. EF1a-driven, Cre-dependent, humanized channelrhodopsin E123T/T159C mutant fused to EYFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Article
    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do
    Promoter
    EF1a
    Tags
    EYFP (Cre-dependent)
    Available since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only

  9. pAAV-EF1a-C-CreintG (AAV1)

    Viral Prep
    #69571-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-EF1a-C-CreintG (#69571). In addition to the viral particles, you will also receive purified pAAV-EF1a-C-CreintG plasmid DNA. EF1a-driven expression of optimized C-terminal component of Cre recombinase dependent on GFP (Cre-DOG OPT); to be coinjected with pAAV-EF1a-N-CretrcintG (Addgene catalog# 69570) and GFP (or for use with transgenic GFP animals). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Connie Cepko
    Article
    Tang et al Nat Neurosci. 2015 Sep;18(9):1334-41. do
    Promoter
    EF1a
    Tags
    None
    Available since
    Feb. 19, 2020
    Availability
    Academic Institutions and Nonprofits only

  10. pAAV-EF1a-N-CretrcintG (AAV1)

    Viral Prep
    #69570-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-EF1a-N-CretrcintG (#69570). In addition to the viral particles, you will also receive purified pAAV-EF1a-N-CretrcintG plasmid DNA. EF1a-driven expression of optimized N-terminal component of Cre recombinase dependent on GFP (Cre-DOG OPT); to be coinjected with pAAV-EF1a-C-CreintG (Addgene catalog# 69571) and GFP (or for use with transgenic GFP animals). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Connie Cepko
    Article
    Tang et al Nat Neurosci. 2015 Sep;18(9):1334-41. do
    Promoter
    EF1a
    Tags
    None
    Available since
    Feb. 19, 2020
    Availability
    Academic Institutions and Nonprofits only

  11. pAAV-CaMKIIa-hChR2(E123A)-mCherry (AAV9)

    Viral Prep
    #35506-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-CaMKIIa-hChR2(E123A)-mCherry (#35506). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hChR2(E123A)-mCherry plasmid DNA. CaMKIIa-driven, humanized channelrhodopsin E123A mutant fused to mCherry for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Article
    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do
    Promoter
    CaMKII
    Tags
    mCherry
    Available since
    March 30, 2018
    Availability
    Academic Institutions and Nonprofits only

  12. pAAV.GFAP.SF-iGluSnFR.A184S (AAV1)

    Viral Prep
    #106192-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV.GFAP.SF-iGluSnFR.A184S (#106192). In addition to the viral particles, you will also receive purified pAAV.GFAP.SF-iGluSnFR.A184S plasmid DNA. GFAP-driven expression of SF-iGluSnFR.A184S. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do
    Promoter
    GFAP
    Tags
    None
    Available since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only

  13. RepX_pET28a(+)

    Plasmid
    #172199
    Purpose
    Expresses 6xHis-tagged RepX in E. coli BL21(DE3), kanamycin resistance, optimal growth at 20 degrees.
    Depositor
    Taekjip Ha
    Article
    Arslan et al Science. 2015 Apr 17;348(6232):344-7. do
    Insert
    RepX superhelicase
    Use
    Tags
    6xHis tag
    Expression
    Bacterial
    Mutation
    C18L, C43S, C167V, S400C, C612A
    Promoter
    Available since
    March 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    172199-plasmid-map-sequence-id-356977

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  15. pSR78

    Plasmid
    #90210
    Purpose
    Expresses the light sensors CcaS and Cph8 as well as the phycocyanobilin biosynthesis genes Ho1 and PcyA
    Depositor
    Jeffrey Tabor
    Article
    Olson et al Mol Syst Biol. 2017 Apr 24;13(4):926. do
    Inserts
    CcaS
    Cph8
    Ho1
    PcyA
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    90210-plasmid-map-sequence-id-178317

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Showing: 821 - 840 of 8082 results