We narrowed to 1,610 results for: Flp

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pAAV-VTKD5-V5-APEX2-NES

Plasmid

#178699

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-OFF) of V5-APEX2-NES in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

V5-APEX2-NES

Use

AAV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF Coff/Fon DREADD Gq-mCherry

Plasmid

#183534

Purpose

INTRSECT Chemogenetics - expresses DREADD Gq-mCherry in cells expressing Flp but not Cre

Depositor

Karl Deisseroth

Insert

DREADD Gq-mCherry

Use

AAV

Mutation

None

Promoter

nEF

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF Con/Fon DREADD Gq-mCherry

Plasmid

#183532

Purpose

INTRSECT Chemogenetics - expresses DREADD Gq-mCherry in cells expressing Cre AND Flp

Depositor

Karl Deisseroth

Insert

DREADD Gq-mCherry

Use

AAV

Mutation

None

Promoter

nEF

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF Con/Foff DREADD Gq-mCherry

Plasmid

#183533

Purpose

INTRSECT Chemogenetics - expresses DREADD Gq-mCherry in cells expressing Cre but not Flp

Depositor

Karl Deisseroth

Insert

DREADD Gq-mCherry

Use

AAV

Mutation

None

Promoter

nEF

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO/Flag-mKO2

Plasmid

#110371

Purpose

Mammalian expression of mKO2 with FLAG tag, Flp-In T-Rex system

Depositor

Sandra Martha Gomes Dias

Insert

monomeric Kusabira Orange 2

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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TRE-FRT-MCS-WPRE-SV40 - GCH27

Plasmid

#230828

Purpose

AAV cloning vector for Flpo-dependent expression under the TRE promoter.

Depositor

Kimberly Ritola

Type

Empty backbone

Use

AAV; Cloning vector, flp/frt

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO/Flag-tGFP

Plasmid

#110375

Purpose

Mammalian expression of turboGFP with FLAG tag, Flp-In T-Rex system

Depositor

Sandra Martha Gomes Dias

Insert

turboGFP

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pMOD4-mT2A-mutated_rpsL-BSD-F

Plasmid

#182338

Purpose

Template plasmid for PCR amplification of initial recombineering cassette

Depositor

Mario Capecchi

Insert

BSD

Use

Mouse Targeting; Flp / frt

Expression

Bacterial

Available Since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

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pTO_CHMP4B_LAP

Plasmid

#101850

Purpose

Tet-inducible expression of human CHMP4B with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integration

Depositor

Daniel Gerlich

Article

Mierzwa et al Nat Cell Biol. 2017 Jul;19(7):787-798. d

Insert

Charged Multivesicular Body Protein 4B (CHMP4B Human)

Use

Gateway cloning, flp-frt recombination, tet-induc…

Tags

Localization and Affinity Purification (LAP) tag

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Dec. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pTO_CHMP2B_LAP

Plasmid

#101848

Purpose

Tet-inducible expression of human CHMP2B with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integration

Depositor

Daniel Gerlich

Article

Mierzwa et al Nat Cell Biol. 2017 Jul;19(7):787-798. d

Insert

CHMP2B (CHMP2B Human)

Use

Flp-frt recombination, tet-inducible expression

Tags

Localization and Affinity Purification (LAP) tag

Expression

Bacterial and Mammalian

Mutation

silent mutation in Thr 104; A to T

Promoter

CMV

Available Since

Dec. 12, 2017

Availability

Academic Institutions and Nonprofits only

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mT2A-tdTomato-FNF

Plasmid

#182340

Purpose

Recombineering cassette plasmid for T2A-tdTomato-FNF

Depositor

Mario Capecchi

Insert

T2A-tdTomato-FNF

Use

Mouse Targeting; Flp / frt

Expression

Mammalian

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TRE3GS-eGFP

Plasmid

#252871

Purpose

TRE3GS expression plasmid for Flp/FRT recombination expressing eGFP

Depositor

Norman Davey

Insert

eGFP

Expression

Mammalian

Promoter

TRE3GS

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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F3 Neo* F3

Plasmid

#140235

Purpose

F3 (FRT variant)-flanked pPgk-pEM7-Neo*-bpA positive selection cassette

Depositor

Mario Capecchi

Insert

Neo*

Use

Mouse Targeting; Flp/ frt

Expression

Bacterial and Mammalian

Available Since

Nov. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Con/Fon DREADD Gi-mCherry

Plasmid

#177672

Purpose

Expresses DREADD hM4D (Gi) in Cre and Flp expressing cells

Depositor

Karl Deisseroth

Has Service

AAV8

Insert

DREADD Gi-mCherry

Use

AAV, Cre/Lox, and Mouse Targeting ; Intrsect

Tags

mCherry

Expression

Mammalian

Promoter

nEF

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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H2B-sfGFP-T2A-iCre

Plasmid

#178546

Purpose

pCMV-Histone H2B-sfGFP-T2A-iCre-bpA-FRT-Neo*-FRT

Depositor

Mario Capecchi

Insert

Histone H2B-sfGFP-T2A-iCre

Use

Cre/Lox and Mouse Targeting; Flp/frt

Expression

Mammalian

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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mT2A-td-sfGFP-FNF

Plasmid

#182339

Purpose

Recombineering cassette plasmid for T2A-td-sfGFP-FNF

Depositor

Mario Capecchi

Insert

T2A-td-sfGFP-FNF

Use

Mouse Targeting; Flp / frt

Expression

Mammalian

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pRDA_852

Plasmid

#185527

Purpose

Lentiviral vector that enables Cre-mediated expression of two sgRNAs. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.

Depositor

Arlene Sharpe

Inserts

First sgRNA cassette
Second sgRNA cassette

Use

CRISPR, Cre/Lox, and Lentiviral ; Flp/frt

Expression

Mammalian

Promoter

Human U6 and Mouse U6

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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FNF-CAG-L-3xSV40pA-L-tdTomato shuttle vector

Plasmid

#182346

Purpose

PacI-flanked CAG-LSL expression cassette with a FNF for positive selection

Depositor

Mario Capecchi

Insert

tdTomato

Use

Mouse Targeting; Flp / frt

Expression

Mammalian

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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tRECs/3xUAS-FP attB

Plasmid

#220330

Purpose

encodes three Fluorescent Protein FLP-OUT cassettes responsive to three different recombinases (mFLP5, B3 and KD)

Depositor

Erdem Bangi

Inserts

B3RT-STOP-B3RT-TagBFP-3xHA
KDRT-STOP-KDRT-GFP-HSV
FRTm71-STOP-FRTm71-mCherry

Expression

Insect

Promoter

UAS

Available Since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Con/Foff DREADD Gi-mCherry

Plasmid

#177673

Purpose

Expresses DREADD hM4D (Gi) only in Cre expressing cells and not in Flp expressing cells

Depositor

Karl Deisseroth

Has Service

AAV8

Insert

DREADD Gi-mCherry

Use

AAV, Cre/Lox, and Mouse Targeting ; Intrsect

Tags

mCherry

Expression

Mammalian

Promoter

nEF

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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