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pYTK092

Plasmid

#65199

Purpose

Encodes URA3 5' Homology as a Type 8b part to be used in the Dueber YTK system

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

URA3 5' Homology

Expression

Bacterial

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pYTK086

Plasmid

#65193

Purpose

Encodes URA3 3' Homology as a Type 7 part to be used in the Dueber YTK system

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

URA3 3' Homology

Expression

Bacterial

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pSEVAT1Z1A

Plasmid

#58296

Purpose

GeneGuard vector cassette: thyA, R6K COR, Zeta toxin; with ampR as cargo.

Depositor

Tom Ellis

Article

Wright et al ACS Synth Biol. 2014 May 13.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pSEVAD7Z1A

Plasmid

#58301

Purpose

GeneGuard vector cassette: dapA, ColE2 COR, Zeta toxin; with ampR as cargo.

Depositor

Tom Ellis

Article

Wright et al ACS Synth Biol. 2014 May 13.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pYTK093

Plasmid

#65200

Purpose

Encodes LEU2 5' Homology as a Type 8b part to be used in the Dueber YTK system

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

LEU2 5' Homology

Expression

Bacterial

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pYTK087

Plasmid

#65194

Purpose

Encodes LEU2 3' Homology as a Type 7 part to be used in the Dueber YTK system

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

LEU2 3' Homology

Expression

Bacterial

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pYTK049

Plasmid

#65156

Purpose

Encodes I-SceI recognition site as a Type 234 part to be used in the Dueber YTK system

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

I-SceI recognition site

Expression

Bacterial

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pMetEGFP

Plasmid

#216330

Purpose

PMetE promoter driving expression of sfGFP

Depositor

Mark Styczynski

Article

Piorino et al ACS Synth Biol. 2023 Aug 18;12(8):2487-2

Insert

Superfolder green fluorescent protein

Expression

Bacterial

Available Since

May 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pET-6xHis-GFP(-12)

Plasmid

#219420

Purpose

Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate

Depositor

Allie Obermeyer

Article

Ahn et al JACS Au. 2024 Sep 17;4(10):3800-3812. do

Insert

GFP(-12)

Tags

MGHHHHHGG

Expression

Bacterial

Promoter

T7

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pBBR1_DVK_AE(J23106-BCD2-E0040m-B0015)

Plasmid

#197672

Purpose

Expresses GFP under the control of J23106 and BCD2

Depositor

Eric Young

Article

Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pBBR1_DVK_AE(J23100-B0032m-E0040m-B0015)

Plasmid

#197657

Purpose

Expresses GFP under the control of J23100 and B0032m

Depositor

Eric Young

Article

Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBR1_DVK_AE(J23100-B0033m-E0040m-B0015)

Plasmid

#197658

Purpose

Expresses GFP under the control of J23100 and B0033m

Depositor

Eric Young

Article

Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBR1_DVK_AE(J23103-B0032m-E0040m-B0015)

Plasmid

#197663

Purpose

Expresses GFP under the control of J23103 and B0032m

Depositor

Eric Young

Article

Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBR1_DVK_AE(J23103-B0033m-E0040m-B0015)

Plasmid

#197664

Purpose

Expresses GFP under the control of J23103 and B0033m

Depositor

Eric Young

Article

Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBR1_DVK_AE(J23103-B0034m-E0040m-B0015)

Plasmid

#197665

Purpose

Expresses GFP under the control of J23103 and B0034m

Depositor

Eric Young

Article

Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBR1_DVK_AE(J23103-BCD2-E0040m-B0015)

Plasmid

#197666

Purpose

Expresses GFP under the control of J23103 and BCD2

Depositor

Eric Young

Article

Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBR1_DVK_AE(J23106-B0032m-E0040m-B0015)

Plasmid

#197669

Purpose

Expresses GFP under the control of J23106 and B0032m

Depositor

Eric Young

Article

Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBR1_DVK_AE(J23106-B0033m-E0040m-B0015)

Plasmid

#197670

Purpose

Expresses GFP under the control of J23106 and B0033m

Depositor

Eric Young

Article

Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pMYT092

Plasmid

#198865

Purpose

Markerless yeast integration Spacer at Int.8 locus to be used in the Ellis MYT system

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Insert

Int.8 Spacer

Use

Synthetic Biology

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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pMYT012

Plasmid

#180665

Purpose

Encodes pTet4 as a Type 2 part to be used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Insert

pTet4

Use

Synthetic Biology

Available Since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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