...Consortium plasmids that encode the extracellular regions of cell surface receptor and secreted proteins as...expression plasmids that encode the extracellular regions of cell surface receptor and secreted proteins as...AVEXIS (for Avidity-based extracellular interaction screen) in which the extracellular regions of receptor ... Plasmid Collections EXPRESs Plasmids EXtracellular Plasmid RESource (EXPRESs) Consortium You may...inserts that encode the whole ectodomains of cell surface receptors are flanked by two 8 bp cutting enzymes...Zebrafish antibody plasmids P. falciparum merozoite surface protein plasmids P. vivax merozoite protein plasmids...blood stage proteins P. falciparum sporozoite cell surface and secreted proteins Mouse sperm Mouse egg Laboratories...

...Transitions. ACS Sens. 2020 Mar 27;5(3):814-822. Bas Teusink ATP (extracellular) ecAT3.10 extracellular ATP sensor...cytosolic Zn(2+). ACS Chem Biol. 2013 Nov 15;8(11):2366-71. Amy Palmer Zinc Cell surface localized zinc ...Mitochondrial Matrix and Intermembrane Space with the GZnP2 Sensor. ACS Chem Biol. 2018 Jul 20;13(7):1897-...fluorescent voltage sensor to detect neural activity (Ace-mScarlet) A high-speed, bright, red fluorescent voltage...for Cytosolic and Intraorganelle Zn(2+) Imaging. ACS Chem Biol. 2015 Sep 18;10(9):2126-34. Maarten Merkx... ADP based on tetramethylrhodamine-labeled ParM. ACS Chem Biol. 2010 Apr 16;5(4):415-25. Martin Webb ATP...Linkers between a Fluorescent Protein and PDE5alpha. ACS Sens. 2017 Jan 27;2(1):46-51. Tetsuya Kitaguchi cGMP...

...One-Step Cloning and Chromosomal Integration of DNA, ACS Synthetic Biology, 2013. DOI: 10.1021/sb400021j Reprinted...

...modified CRISPR-Cas9 system - Shuo Lin Lab Re-purposed Ac/Ds transgenic system enabling CRISPR/dCas9-interference...receptors or monoclonal antibodies to zebrafish cell surface receptors and secreted proteins. Return to top ...

...higher efficiency and product purity. 2017. Komor AC, Zhao KT, Packer MS, Gaudelli NM, Waterbury AL, Koblan...engineering and protein delivery. 2017. Rees HA, Komor AC, Yeh WH, Caetano-Lopes J, Warman M, Edge ASB, Liu...Cas9-cytidine deaminase fusions. 2017. Kim YB, Komor AC, Levy JM, Packer MS, Zhao KT, Liu DR. Nat Biotechnol...DNA without DNA cleavage. 2017. Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran AH, Bryson DI, Liu DR...without double-stranded DNA cleavage. 2016. Komor AC, Kim YB, Packer MS, Zuris JA, Liu DR. Nature . 533...The target is present immediately adjacent to a P rotospacer A djacent M otif (PAM). The PAM sequence serves...codons that make up a gene PAM P rotospacer A djacent M otif; Sequence adjacent to the target sequence that...

...almost all of these guidelines can apply to any workplace or job. It amazes me when the most analytic scientists...thing to do is not go into these labs in the first place. This is another reason to do your research and ... Wrote blog, Toxic Academic Mentors.  What is workplace bullying? Threats to professional status – including... Scientists love learning and science so find a place that loves you back. Additional resources on the...

...that they may want to consider for future work placements. The chance to learn and collaborate all while...which you can apply to. These structured types of placements often have some form of built-in professional...for success prior to your departure for the new placement. Make sure you read any relevant literature for...

... Controlled CRSIPR-Cas-based Genomic Regulation. ACS 6, 5, 695-703 (2020). https://pubs.acs.org/doi/10.1021...is 3’ phosphorothioate linkage, where a sulfur replaces a nonbridging phosphate oxygen. Other useful backbone...

...enthusiastic, “Our company culture! Addgene is the best place to work, we do SO many fun things!!”  Okay - honestly.... Instead we picniced outside of our new office space on the nicely manicured lawn of University Park ... the difference here at Addgene is the emphasis placed on team building throughout the years. This team... master drywall hanger?  4. Utilize your office space too Balance point number 3 with doing fun things...

...interview with Novartis researcher, Carrie Bhang. The ClonTracer Library, deposited by Carrie Bhang, a research... exposure to the drug discovery process.” The ClonTracer library originally came about as Bhang and co-workers...questions that we could apply [it to].” Using ClonTracer to study drug resistance Although Bhang and co-workers...the publication, Bhang and coworkers used the ClonTracer library to determine whether or not cancer cell...the total population are easily be missed. The ClonTracer library allows a researcher to label individual...cells' genomes upon infection. When using the ClonTracer system, lentiviruses containing these barcodes...is performed via barcode sequencing. Using the ClonTracer library, Bhang et al. found that antibiotic resistant...

...orders that are placed through internal purchasing systems. All orders must be placed directly on our ... questions related to the ordering process: I placed an order through my university’s purchasing system...getting at one bigger question: How does a customer place an Addgene order using a PO number? Why do I have... organization can create an Addgene account and place an order: a purchasing agent, lab manager, or the... the contact email addresses provided. How do I place an order using a PO number? Placing an order on ...you’re using a PO number. Follow these steps to place an order online while using a PO number: Log in...

...occasionally posting something science-related on Facebook. “I think this helped me say with confidence when...

...get to where I was. It was really luck of right place, right time, and having a good interview experience...

...science communication careers, you’re in the right place. For this series, I’ll interview three science communicators...

...They called this editor Target-ACE (adenine and cytosine editor). Target-ACE exhibits increased editing efficiency...mammalian cells. Read the paper in bioRxiv Find Target-ACE   CRISPR/Cas9-based toolkit for epitope tagging ...this toolkit might be used in. Read the paper in ACS Synthetic Biology Find the CRISPRi toolkit SECURE...ribonucleoproteins for transient expression. They replaced the tetraloop of the sgRNA scaffold with a com...

...Chemical Biology 2018. PubMed: PMID 30220597. ACE system: A versatile in vivo fluorescent reporter ...monitors both APOBEC- and Cas9-mediated Editing (ACE) in real-time. The reporter constitutively expresses...fluorescence microscopy or flow cytometry. Using the ACE system, the Harris lab showed that human APOBEC3A-Cas9n-UGI...containing rat APOBEC1. Finally, they show that the ACE reporter can also quantify Cas9 cleavage, thus exhibiting...gene editing activities. Click here to find the ACE reporter plasmid! Martin et al., Nucleic Acids Research...

...College Park, but she quickly realized it wasn’t the place for her. “I went to grad school with the plan of...different directions is sometimes at odds with the space needed to be creative. “You need a lot of time to...Susan feels she was lucky to have been in the right place at the right time when she started her scicomm career...

...mediated editing in real time The Harris lab created ACE, a reporter that monitors APOBEC (a family of cytidine...editing in real time (St. Martin et al., 2018). The ACE reporter is a bicistronic construct that consists... Figure 1: APOBEC- and Cas9-mediated editing (ACE) reporter in action. Image from St. Martin et al....expression to cells expressing eGFP and mCherry. ACE was used to identify cells that have been successfully...and APOBEC3B (St. Martin et al., 2018). TLDR: The ACE reporter from the Harris Lab relies on the restoration...for single base editing by APOBEC-Cas9 Though the ACE reporter quantifies APOBEC-Cas9 efficiency in real-time..., and luciferase. References Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran AH, Bryson DI, Liu DR...

...S, Yetnikoff L, Kalmbach A, Ma T, Ztaou S, Sienna AC, Tepler S, Poulin JF, Ansorge M, Awatramani R, Kang...