...Synthetic Biology.” ACS Synthetic Biology, vol. 5, no. 10, American Chemical Society (ACS), 2 May 2016, pp...Traditional Biology.” ACS Synthetic Biology, vol. 5, no. 1, American Chemical Society (ACS), 4 Nov. 2015, pp...Secretion Libraries.” ACS Synthetic Biology, vol. 6, no. 6, American Chemical Society (ACS), 15 Mar. 2017, ...Multipart Assembly.” ACS Synthetic Biology, vol. 4, no. 9, American Chemical Society (ACS), 1 May 2015, pp...Saccharomyces Cerevisiae.” ACS Synthetic Biology, vol. 12, no. 11, American Chemical Society (ACS), 6 Nov. 2023, ...Toolbox for Plants.” ACS Synthetic Biology, vol. 3, no. 11, American Chemical Society (ACS), 20 Feb. 2014, ...Acinetobacter Baumannii.” ACS Synthetic Biology, vol. 12, no. 9, American Chemical Society (ACS), 16 Aug. 2023, ...

... Controlled CRSIPR-Cas-based Genomic Regulation. ACS 6, 5, 695-703 (2020). https://pubs.acs.org/doi/10.1021...is 3’ phosphorothioate linkage, where a sulfur replaces a nonbridging phosphate oxygen. Other useful backbone...

...that they may want to consider for future work placements. The chance to learn and collaborate all while...which you can apply to. These structured types of placements often have some form of built-in professional...for success prior to your departure for the new placement. Make sure you read any relevant literature for...

...almost all of these guidelines can apply to any workplace or job. It amazes me when the most analytic scientists...thing to do is not go into these labs in the first place. This is another reason to do your research and ... Wrote blog, Toxic Academic Mentors.  What is workplace bullying? Threats to professional status – including... Scientists love learning and science so find a place that loves you back. Additional resources on the...

...orders that are placed through internal purchasing systems. All orders must be placed directly on our ... questions related to the ordering process: I placed an order through my university’s purchasing system...getting at one bigger question: How does a customer place an Addgene order using a PO number? Why do I have... organization can create an Addgene account and place an order: a purchasing agent, lab manager, or the... the contact email addresses provided. How do I place an order using a PO number? Placing an order on ...you’re using a PO number. Follow these steps to place an order online while using a PO number: Log in...

...interview with Novartis researcher, Carrie Bhang. The ClonTracer Library, deposited by Carrie Bhang, a research... exposure to the drug discovery process.” The ClonTracer library originally came about as Bhang and co-workers...questions that we could apply [it to].” Using ClonTracer to study drug resistance Although Bhang and co-workers...the publication, Bhang and coworkers used the ClonTracer library to determine whether or not cancer cell...the total population are easily be missed. The ClonTracer library allows a researcher to label individual...cells' genomes upon infection. When using the ClonTracer system, lentiviruses containing these barcodes...is performed via barcode sequencing. Using the ClonTracer library, Bhang et al. found that antibiotic resistant...

...enthusiastic, “Our company culture! Addgene is the best place to work, we do SO many fun things!!”  Okay - honestly.... Instead we picniced outside of our new office space on the nicely manicured lawn of University Park ... the difference here at Addgene is the emphasis placed on team building throughout the years. This team... master drywall hanger?  4. Utilize your office space too Balance point number 3 with doing fun things...

...occasionally posting something science-related on Facebook. “I think this helped me say with confidence when...

...get to where I was. It was really luck of right place, right time, and having a good interview experience...

...science communication careers, you’re in the right place. For this series, I’ll interview three science communicators...

...They called this editor Target-ACE (adenine and cytosine editor). Target-ACE exhibits increased editing efficiency...mammalian cells. Read the paper in bioRxiv Find Target-ACE   CRISPR/Cas9-based toolkit for epitope tagging ...this toolkit might be used in. Read the paper in ACS Synthetic Biology Find the CRISPRi toolkit SECURE...ribonucleoproteins for transient expression. They replaced the tetraloop of the sgRNA scaffold with a com...

...Chemical Biology 2018. PubMed: PMID 30220597. ACE system: A versatile in vivo fluorescent reporter ...monitors both APOBEC- and Cas9-mediated Editing (ACE) in real-time. The reporter constitutively expresses...fluorescence microscopy or flow cytometry. Using the ACE system, the Harris lab showed that human APOBEC3A-Cas9n-UGI...containing rat APOBEC1. Finally, they show that the ACE reporter can also quantify Cas9 cleavage, thus exhibiting...gene editing activities. Click here to find the ACE reporter plasmid! Martin et al., Nucleic Acids Research...

...College Park, but she quickly realized it wasn’t the place for her. “I went to grad school with the plan of...different directions is sometimes at odds with the space needed to be creative. “You need a lot of time to...Susan feels she was lucky to have been in the right place at the right time when she started her scicomm career...

...mediated editing in real time The Harris lab created ACE, a reporter that monitors APOBEC (a family of cytidine...editing in real time (St. Martin et al., 2018). The ACE reporter is a bicistronic construct that consists... Figure 1: APOBEC- and Cas9-mediated editing (ACE) reporter in action. Image from St. Martin et al....expression to cells expressing eGFP and mCherry. ACE was used to identify cells that have been successfully...and APOBEC3B (St. Martin et al., 2018). TLDR: The ACE reporter from the Harris Lab relies on the restoration...for single base editing by APOBEC-Cas9 Though the ACE reporter quantifies APOBEC-Cas9 efficiency in real-time..., and luciferase. References Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran AH, Bryson DI, Liu DR...

...a red-shifted Ace-mScarlet, a fusion of a voltage-sensitive inhibitory rhodopsin (Ace2N) with the red ...Figure 2: Ace-mScarlet is a fusion between the voltage-sensitive inhibitory rhodopsin Ace2N and mScarlet...seven or eight amino acid linker between them. The Ace-mScarlet voltage indicator works like many FRET-opsin... an increase in the neuron’s membrane potential, Ace absorbance increases and results in higher quenching...blue-light-sensitive channelrhodopsins. Find the Ace-mScarlet voltage indicator plasmids at Addgene! Beck...mScarlet. Ace2N quenches a proportion of mScarlet's emission via FRET. Image from Beck and Gong, 2019. ...

...updating this article. References Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran AH, Bryson DI, Liu DR... from this publication at Addgene. Kim YB, Komor AC, Levy JM, Packer MS, Zhao KT, Liu DR (2017) Increasing...plasmids from this publication at Addgene. Komor AC, Kim YB, Packer MS, Zuris JA, Liu DR (2016) Programmable...plasmids from this publication at Addgene. Komor AC, Zhao KT, Packer MS, Gaudelli NM, Waterbury AL, Koblan...from this publication at Addgene. Rees HA, Komor AC, Yeh W-H, Caetano-Lopes J, Warman M, Edge ASB, Liu...the base edit. Cytidine deamination takes place on the free strand of DNA and converts a C to U ..., ABE8s resulted in 1.5-fold more editing at protospacer positions A5-A7 and 3.2-fold more editing at ...

...the ACS career fair is more industry-focused. For another industry-geared option, try the Biospace career...Biology (ASCB) or the American Chemical Society (ACS) are just 2 examples of organizations that hold large...best options are the Naturejobs Career Expo and the ACS career fair, which are larger meetings offering resources...Similar to a conference, job fairs bring you face-to-face with working professionals, with the added benefit...career events page. Biospace holds career events all over the country; sometimes several per month, giving... online career resources Once you’ve identified places you’d like to work, get online and take a look ...know, where they are, and who they know all in one place! This information can be critical to a focused and...

...spheroplasts to release encapsulated proteins. Video by ACS. For a more cartoony explanation of R-bodies, check... Breaks Membranes to Release Encapsulated Cargo. ACS Synth. Biol. (2016). doi:10.1021/acssynbio.5b00237...penetrate other barriers (like vasculature or extracellular matrices) in response to pH changes. Beyond...functionalized so that they adhere to specific surfaces, or they could be decorated with enzymes for use...

.... Created with BioRender.com In their recent ACS Synthetic Biology publication, Mary Dunlop’s lab ...cut in as little as 2 hours.  Sheets, MB, et al., ACS Synth Biol. 2020. https://pubs.acs.org/doi/10.1021...