We narrowed to 301,213 results for: addgene

1. pMC-ME_T2A-BFP-P2A-NTRv2-pA ORF-1

   Plasmid

   #200539

   Purpose

   mini circle middle entry plasmid for T2A- BFP- P2A- ntr version 2- pA (ORF 1) with flanking BsaI cut sites

   Depositor

   Junsu Kang

   Insert

   BFP, NTRv2
   

   Use

   Mini-golden

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMC-ME_T2A-BFP-P2A-NTRv2-pA ORF-2

   Plasmid

   #200540

   Purpose

   mini circle middle entry plasmid for T2A- BFP- P2A- ntr version 2- pA (ORF 2) with flanking BsaI cut sites

   Depositor

   Junsu Kang

   Insert

   BFP, NTRv2
   

   Use

   Mini-golden

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMC-ME_T2A-BFP-P2A-NTRv2-pA ORF-3

   Plasmid

   #200541

   Purpose

   mini circle middle entry plasmid for T2A- BFP- P2A- ntr version 2- pA (ORF 3) with flanking BsaI cut sites

   Depositor

   Junsu Kang

   Insert

   BFP, NTRv2
   

   Use

   Mini-golden

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMC-ME_CreER-pA-FRT-acry:mCherry-pA-FRT

   Plasmid

   #200544

   Purpose

   mini circle middle entry plasmid for CreER-pA-FRT-acry: mCherry -pA-FRT with flanking BsaI cut sites

   Depositor

   Junsu Kang

   Insert

   CreER, mCherry
   

   Use

   Mini-golden

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMC-ME T2A-mCherry-P2A-NTRv2-pA ORF-1

   Plasmid

   #200529

   Purpose

   mini circle middle entry plasmid for T2A- mCherry- P2A- ntr version 2- pA (ORF 1) with flanking BsaI cut sites

   Depositor

   Junsu Kang

   Insert

   mCherry, NTRv2
   

   Use

   Mini-golden

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMC-ME T2A-mCherry-P2A-NTRv2-pA ORF-2

   Plasmid

   #200530

   Purpose

   mini circle middle entry plasmid for T2A- mCherry- P2A- ntr version 2- pA (ORF 2) with flanking BsaI cut sites

   Depositor

   Junsu Kang

   Insert

   mCherry, NTRv2
   

   Use

   Mini-golden

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMC-ME_T2A-mCherry-P2A-NTRv2-pA ORF-3

   Plasmid

   #200531

   Purpose

   mini circle middle entry plasmid for T2A- mCherry- P2A- ntr version 2- pA (ORF 3) with flanking BsaI cut sites

   Depositor

   Junsu Kang

   Insert

   mCherry, NTRv2
   

   Use

   Mini-golden

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pENTR-mCherry.OVA(323-339)

   Plasmid

   #190006

   Purpose

   Gateway entry vector for the expression of mCherry and OVA323-339 peptide fusion

   Depositor

   Siyuan Zhang

   Insert

   mCherry.OVA(323-339)
   

   Use

   Synthetic Biology

   Tags

   OVA(323-339)

   Expression

   Mutation

   Promoter

   Available since

   July 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. cTRE-LSL-GFP-IRES-Cas9-sgPtenX1.1

   Plasmid

   #135669

   Purpose

   Introduce Dox/Cre-inducible (TRE promoter followed by lox-stop-lox) Cas9 cDNA plus Pten-targeting sgRNA into (ES) cells by recombination-mediated cassette exchange

   Depositor

   Florian Karreth

   Insert

   sgPten
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pE4F1.1.0-gDNA

    Plasmid

    #132451

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    E4F1 (E4F1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSBbi-Pur-LPAR

    Plasmid

    #125205

    Purpose

    SB-transposon plasmid for stable expression of lipid raft-targeted FRET-based kinase activity reporter with EV spacer, with AarI cassette for cloning of a kinase-target peptide

    Depositor

    Eric Davis

    Insert

    Lyn-Phospho-Activity-Reporter, Empty
    

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. 5K29 (PP-BRD3)

    Plasmid

    #101883

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PP-BRD3
    

    Use

    Tags

    6xHis, TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 30, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. 5TCM (PP-BRD3)

    Plasmid

    #101888

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PP-BRD3
    

    Use

    Tags

    6xHis, TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 30, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. 4QNS

    Plasmid

    #61957

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PF08_0034:MAC04J-B11:C229648
    

    Use

    Tags

    MHHHHHHSSGRENLYFQG

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7-lacO

    Available since

    March 2, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

15. PP-AHA1

    Plasmid

    #28123

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PFC0360w:MAC03H-A08:C212775 (PFC0360w Plasmodium falciparum)
    

    Use

    Tags

    His tag with TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 28, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

16. pUC18

    Plasmid

    #50004

    Purpose

    pUC cloning vector

    Depositor

    Joachim Messing

    Article

    Norrander et al Gene. 1983 Dec;26(1):101-6.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSL1423

    Plasmid

    #194904

    Purpose

    pSLI-TGD/TurboID, C-terminal tag of recodonized TurboID for Plasmodium falciparum

    Depositor

    Scott Lindner

    Insert

    TurboID
    

    Use

    Plasmodium falciparum

    Tags

    GFPmut2

    Expression

    Mutation

    Promoter

    None. This allows a C-terminal fusion to a protei…

    Available since

    Feb. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. PANK3

    Plasmid

    #32872

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PANK3 (PANK3 Human)
    

    Use

    Tags

    His tag (with Thrombin cleavage)

    Expression

    Bacterial

    Mutation

    amino acids 10-370 only

    Promoter

    Available since

    Jan. 6, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

19. pINDUCER20-FLAG-hMYOD1 (#800)

    Plasmid

    #78330

    Purpose

    Doxycycline-inducible lentiviral overexpression of human MYOD1 ORF with a FLAG tag

    Depositor

    Matthew Alexander, Louis Kunkel

    Insert

    MYOD1 (MYOD1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    TRE2

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)

    Plasmid

    #140082

    Purpose

    Entry cloning vector for in vitro transcription or expression of SpCas9 sgRNAs from a T7 promoter

    Depositor

    Keith Joung

    Insert

    pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)
    

    Use

    In vitro transcription (mrna synthesis)

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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