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AAEL009024 PDF R

Plasmid

#115408

Purpose

Expresses Ae. aegypti receptor in mammalian cells

Depositor

Leslie Vosshall

Article

Duvall et al Cell. 2019 Feb 7;176(4):687-701.e5. doi:

Insert

AAEL009024 PDF R (LOC5571400 Aedes aegypti)

Expression

Mammalian

Available Since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pET21a_Msm_RbpA

Plasmid

#87928

Purpose

Mycobacterium smegmatis RpbA in pET21a (ampR) used to co-express with Msm SigA for crystallization purposes

Depositor

Seth Darst

Article

Hubin et al Elife. 2017 Jan 9;6. pii: e22520. doi: 1

Insert

RbpA

Expression

Bacterial

Promoter

T7P

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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CS2-GFP-nopo

Plasmid

#47964

Purpose

Expression of Fly nopo

Depositor

Laura Lee

Article

Merkle et al Development. 2009 Feb;136(3):449-59. doi

Insert

no poles (nopo Fly)

Tags

GFP

Expression

Mammalian

Available Since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3-cdc2-AF (718)

Plasmid

#39872

Depositor

Jonathon Pines

Article

Hagting et al EMBO J. 1998 Jul 15;17(14):4127-38.

Insert

Cdc2 (CDK1 Human)

Expression

Mammalian

Mutation

AF = T14A, Y15F

Promoter

CMV

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only
LXRalpha_pcDNA3.1

Plasmid

#177621

Purpose

Full-length human LXR alpha cDNA sequence in pcDNA3.1+

Depositor

Thomas Burris

Article

Griffett et al ACS Chem Biol. 2022 Apr 13. doi: 10.1021

Insert

LXR alpha (NR1H3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2+ H2B-mCherry

Plasmid

#99265

Purpose

Contains a fusion of human histone H2B to fluorescent protein mCherry in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.

Depositor

Philipp Keller

Article

Wan et al Cell. 2019 Oct 3;179(2):355-372.e23. doi

Insert

mCherry

Tags

H2B

Expression

Mammalian

Promoter

SP6

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pmNeon Green-N1

Plasmid

#202429

Purpose

Mammalian expression vector containing mNeonGreen

Depositor

David Bryant

Article

Sandilands et al J Cell Biol. 2023 Apr 3;222(4):e20220611

Type

Empty backbone

Use

Lentiviral

Available Since

March 3, 2025

Availability

Academic Institutions and Nonprofits only

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CFP-C1-PLCdelta-PH

Plasmid

#21262

Depositor

Tobias Meyer

Article

Botelho et al J Cell Biol. 2000 Dec 25. 151(7):1353-68

Insert

PLCdelta (PLCD1 Human)

Tags

CFP

Expression

Mammalian

Mutation

PH domain of PLCdelta (human). Also known as PLCd…

Available Since

July 10, 2009

Availability

Academic Institutions and Nonprofits only

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LOC5567066 Gastrin-releasing peptide receptor

Plasmid

#115372

Purpose

Expresses Ae. aegypti receptor in mammalian cells

Depositor

Leslie Vosshall

Article

Duvall et al Cell. 2019 Feb 7;176(4):687-701.e5. doi:

Insert

LOC5567066 Gastrin-releasing peptide receptor (LOC5567066 Aedes aegypti)

Expression

Mammalian

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pJFRC7-UAS-NPRR_ANP

Plasmid

#133243

Purpose

Fluorescent reporter for neuropeptide release imaging

Depositor

David Anderson

Article

Ding et al Elife. 2019 Jun 26;8. pii: 46421. doi: 1

Insert

rat atrial natriuretic peptide-fused codon-optimized GCaMP6s

Expression

Insect

Promoter

hsp70

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pBTK1023 (pDS-InR1)

Plasmid

#183130

Purpose

Expresses dsRNA targeting the InR1 gene from Apis mellifera

Depositor

Jeffrey Barrick

Article

Leonard et al Science. 2020 Jan 31;367(6477):573-576.

Insert

Fragment of InR1 gene

Use

Synthetic Biology

Available Since

May 19, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pFL-V5-GW

Plasmid

#78857

Purpose

DULIP Prey Destination Vector (Gateway, N-terminal)

Depositor

Erich Wanker

Article

Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

Type

Empty backbone

Use

Gateway Cloning

Tags

Firefly luciferase, V5-tag

Expression

Mammalian

Promoter

CMV

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pPA-RL-GW

Plasmid

#78855

Purpose

DULIP Bait Destination Vector (Gateway, N-terminal)

Depositor

Erich Wanker

Article

Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

Type

Empty backbone

Use

Gateway Cloning

Tags

Protein A-Renilla luciferase

Expression

Mammalian

Promoter

CMV

Available Since

July 7, 2016

Availability

Academic Institutions and Nonprofits only

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pGW-FL-V5

Plasmid

#78858

Purpose

DULIP Prey Destination Vector (Gateway, C-terminal)

Depositor

Erich Wanker

Article

Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

Type

Empty backbone

Use

Gateway Cloning

Tags

Firefly luciferase, V5-tag

Expression

Mammalian

Promoter

CMV

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pGW-RL-PA

Plasmid

#78856

Purpose

DULIP Bait Destination Vector (Gateway, C-terminal)

Depositor

Erich Wanker

Article

Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

Type

Empty backbone

Use

Gateway Cloning

Tags

Renilla luciferase, Protein A

Expression

Mammalian

Promoter

CMV

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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CS2-GFP-Ben

Plasmid

#47966

Purpose

Expression of fly Ben

Depositor

Laura Lee

Article

Merkle et al Development. 2009 Feb;136(3):449-59. doi

Insert

Bendless (ben Fly)

Tags

GFP

Expression

Mammalian

Available Since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only
pKLV-U6gRNA(BbsI)-PGKpuro2ABFP-L1mono3

Plasmid

#73542

Purpose

Expression of sgRNA targeting LINE-1 and TagBFP

Depositor

Constance Ciaudo

Article

Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

Insert

LINE-1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV-U6gRNA(BbsI)-PGKpuro2ABFP-L1mono1

Plasmid

#73543

Purpose

Expression of sgRNA targeting LINE-1 and TagBFP

Depositor

Constance Ciaudo

Article

Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

Insert

LINE-1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-T11HA-hMOV10-CD

Plasmid

#178908

Purpose

expression of catalytic dead hMOV10 K530A

Depositor

Constance Ciaudo

Article

Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

Insert

cDNA Catalytic dead hMOV10 (MOV10 Human)

Tags

T11 beta strand of GFP-HA

Expression

Mammalian

Mutation

K530A catalytic dead AAG>GCA

Promoter

CMV protmoter

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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Soat2_Luc

Plasmid

#177623

Purpose

Cloned human Soat2 promoter containing exon1 and 2 coupled to luciferase

Depositor

Thomas Burris

Article

Griffett et al ACS Chem Biol. 2022 Apr 13. doi: 10.1021

Insert

human Soat2

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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