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We narrowed to 5,317 results for: golden gate

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Showing: 821 - 840 of 5317 results

  1. C39m

    Plasmid
    #120972
    Purpose
    MoClo golden gate assembly CD part for ccdB (DNA gyrase toxin, modified synonymously to remove BsaI cut site; ccdA/C79m is antitoxin). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ccdB toxin
    Use
    Synthetic Biology
    Mutation
    BsaI site removed
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120972-plasmid-map-sequence-id-236024

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  2. P9m

    Plasmid
    #120937
    Purpose
    MoClo golden gate assembly AB part for pOR1-OR2 (lambda cI-repressable strong promoter) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    Pr-OR1-OR2
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120937-plasmid-map-sequence-id-247706

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  3. pGGG-TaBSS2-GUS(int)

    Plasmid
    #242017
    Purpose
    L2 Golden Gate construct for in planta (wheat) expression of transgenic GUS (with introns) driven by the Basal Spikelet Specific 2 regulatory environment.
    Depositor
    Cristobal Uauy
    Insert
    GUS_with_introns
    Expression
    Plant
    Promoter
    BSS2
    Available since
    March 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    242017-plasmid-map-sequence-id-504531

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  4. pGGG-TaBSS2-TaSEP1B6(int)

    Plasmid
    #242016
    Purpose
    L2 Golden Gate construct for in planta (wheat) expression of cisgenic SEP1-B6 driven by the Basal Spikelet Specific 2 regulatory environment.
    Depositor
    Cristobal Uauy
    Insert
    TaSEP1-B6_with_introns
    Tags
    3x FLAG
    Expression
    Plant
    Promoter
    BSS2
    Available since
    March 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    242016-plasmid-map-sequence-id-504530

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  5. pGGG-TaBSS1-GUS(int)

    Plasmid
    #242013
    Purpose
    L2 Golden Gate construct for in planta (wheat) expression of transgenic GUS (with introns) driven by the Basal Spikelet Specific 1 regulatory environment.
    Depositor
    Cristobal Uauy
    Insert
    GUS_with_introns
    Expression
    Plant
    Promoter
    BSS1
    Available since
    March 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    242013-plasmid-map-sequence-id-504513

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  6. pGGG-TaBSS1-TaSEP1B6(int)

    Plasmid
    #242012
    Purpose
    L2 Golden Gate construct for in planta (wheat) expression of cisgenic SEP1-B6 driven by the Basal Spikelet Specific 1 regulatory environment.
    Depositor
    Cristobal Uauy
    Insert
    TaSEP1-B6_with_introns
    Expression
    Plant
    Promoter
    BSS1
    Available since
    March 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    242012-plasmid-map-sequence-id-504511

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  7. pGGG-TaBSS2-TaMOFB1(int)

    Plasmid
    #242015
    Purpose
    L2 Golden Gate construct for in planta (wheat) expression of cisgenic MOF-B1 driven by the Basal Spikelet Specific 2 regulatory environment.
    Depositor
    Cristobal Uauy
    Insert
    TaMOF-B1_with_introns
    Tags
    3x FLAG
    Expression
    Plant
    Promoter
    BSS2
    Available since
    March 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    242015-plasmid-map-sequence-id-504508

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  8. pGGG-TaBSS1-TaMOFB1(int)

    Plasmid
    #242011
    Purpose
    L2 Golden Gate construct for in planta (wheat) expression of cisgenic MOF-B1 driven by the Basal Spikelet Specific 1 regulatory environment.
    Depositor
    Cristobal Uauy
    Insert
    TaMOF-B1_with_introns
    Expression
    Plant
    Promoter
    BSS1
    Available since
    March 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    242011-plasmid-map-sequence-id-504506

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  9. U4m

    Plasmid
    #121017
    Purpose
    MoClo golden gate assembly BC part for UTR1 (very strong RBS). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    UTR1
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121017-plasmid-map-sequence-id-236179

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  10. pAGM4723:Ble:Cas9

    Plasmid
    #112208
    Purpose
    Golden Gate Level 2 construct encoding Cas9-YFP, Zeocin resistance.
    Depositor
    Thomas Mock
    Inserts
    Cas9
    SheBle
    Use
    CRISPR; Diatom, phaeodactylum
    Tags
    Phaeodactylum fcp promoter and terminator and YFP
    Mutation
    Phaeodactylum Fcp promoter
    Promoter
    Phaeodactylum Fcp
    Available since
    July 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    112208-plasmid-map-sequence-id-217248

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  11. pSPIN-GG

    Plasmid
    #251750
    Purpose
    Single-plasmid V. cholerae CAST encoding proteins, crRNA, and donor DNA. Entry vector: GFP dropout; BbsI spacer, BsaI promoter swap, SAPI donor cloning; pSC101* backbone.
    Depositor
    Thomas Gorochowski
    Publication
    Irvine et al Methods Mol Biol. 2026;3041:33-45. doi:
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23119
    Available since
    Aug. 18, 2026
    Availability
    Academic Institutions and Nonprofits only
    251750-plasmid-map-sequence-id-514588

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  14. p028g13-MBP

    Plasmid
    #249631
    Purpose
    Clone MBP polypeptide in the N-terminal of a protein to be expressed using pET28g backbone, using fusion sites 1 and 3
    Depositor
    Isabel Abreu
    Publication
    Luis et al PLoS One. 2025 Jul 7;20(7):e0327341. doi
    Insert
    N-terminal tag with MBP polypeptide
    Expression
    Bacterial
    Mutation
    Silent mutation on Gly-20 to remove a BsaI recogn…
    Available since
    April 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    249631-plasmid-map-sequence-id-504970

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  15. pNDGG005

    Plasmid
    #231309
    Purpose
    Vector part for BEVA; BEVA2.0 Position 3 RSF1010 broad host range origin of replication with oriT, CmR.
    Depositor
    Barney Geddes
    Publication
    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi
    Insert
    BEVA2.0 Position 3 RSF1010 broad host range origin of replication with oriT, CmR.
    Expression
    Bacterial
    Available since
    May 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    231309-plasmid-map-sequence-id-472146

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  16. pNDGG006

    Plasmid
    #231310
    Purpose
    Vector part for BEVA; BEVA2.0 Position 3 p15A narrow host range origin of replication with oriT, CmR.
    Depositor
    Barney Geddes
    Publication
    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi
    Insert
    BEVA2.0 Position 3 p15A narrow host range origin of replication with oriT, CmR.
    Expression
    Bacterial
    Available since
    March 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    231310-plasmid-map-sequence-id-463781

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  17. pNDGG007

    Plasmid
    #231311
    Purpose
    Vector part for BEVA; BEVA2.0 Position 3 pMB1 narrow broad host range origin of replication with oriT, CmR.
    Depositor
    Barney Geddes
    Publication
    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi
    Insert
    BEVA2.0 Position 3 pMB1 narrow broad host range origin of replication with oriT, CmR.
    Expression
    Bacterial
    Available since
    March 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    231311-plasmid-map-sequence-id-463780

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Showing: 821 - 840 of 5317 results