Addgene

We narrowed to 26,637 results for: nts

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pYO734

Plasmid

#235753

Purpose

Protein expression of ScVPS30 and ZZ-ScVPS38

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Inserts

VPS30 (VPS30 Budding Yeast)
VPS38 (VPS38 Budding Yeast)

Use

Tags

ZZ-3xTEV

Expression

Yeast

Mutation

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pYO934

Plasmid

#235766

Purpose

Expression of ScVPS34 delta CBR1 L31-D55-3xFLAG undeer its own promoter

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS34 delta CBR1

Use

Tags

3xFLAG

Expression

Yeast

Mutation

aa 31-55 deleted

Promoter

ScVPS34 promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pYO760

Plasmid

#235759

Purpose

Expression of ScVPS38 delta C2 (1-154)-x3FLAG under its own promoter

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS38 delta C2

Use

Tags

3xFLAG

Expression

Yeast

Mutation

aa 1-154 deleted

Promoter

ScVPS38 promoter

Available since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pYO761

Plasmid

#235760

Purpose

Expression of ScVPS38 delta BARA2 (320-440)-x3FLAG under its own promoter

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VSP38 delta BARA2

Use

Tags

3xFLAG

Expression

Yeast

Mutation

aa 320-440 deleted

Promoter

ScVPS38 promoter

Available since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pYO765

Plasmid

#235762

Purpose

Expression of ScVPS38 delta C2 (1-154) -EGFP under its own promoter

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS38

Use

Tags

EGFP

Expression

Yeast

Mutation

aa 1-154 deleted

Promoter

ScVPS38 promoter

Available since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pYO766

Plasmid

#235763

Purpose

Expression of ScVPS38 delta BARA2 (320-440)-EGFP under its own promoter

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VSP38 delta BARA2

Use

Tags

EGFP

Expression

Yeast

Mutation

aa 320-440 deleted

Promoter

ScVPS38 promoter

Available since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pYO933

Plasmid

#235765

Purpose

Expression of ScVPS34 delta C154-V202-3xFLAG under its own promoter

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS34 delta C154-V202

Use

Tags

3xFLAG

Expression

Yeast

Mutation

aa 154-202 deleted

Promoter

ScVPS34 promoter

Available since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pNP1047

Plasmid

#222726

Purpose

pNP555 - IS621 RE-LE TBL-T5, DBL-WT bRNA in cis

Depositor

Patrick Hsu

Article

Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

Insert

IS621 bridge RNA

Use

Tags

sfGFP

Expression

Bacterial

Mutation

Promoter

Available since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-PGUS

Plasmid

#207827

Purpose

FLAG-tagged PGUS driven by CMV promoter

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

PGUS

Use

Lentiviral

Tags

FLAG

Expression

Mutation

Promoter

CMV

Available since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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BPK617

Plasmid

#179297

Purpose

Expresses dCas9 fused to VP64

Depositor

Keith Joung

Article

Tak et al Nat Methods. 2021 Sep;18(9):1075-1081. d

Insert

dCas9-VP64

Use

Tags

Expression

Mammalian

Mutation

D10A, H840A (catalytically inactive Cas9)

Promoter

CAG

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pSUMO chicken-cPLA2-C2-domain

Plasmid

#170753

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Hirano et al Elife. 2019 May 3;8. pii: 44760. doi: 10

Insert

PLA2G4A-C2 domain (PLA2G4A Chicken)

Use

Tags

SUMO

Expression

Bacterial

Mutation

Promoter

Available since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCV-HA-shift-IRES-GFP(KaganE21)

Plasmid

#52004

Purpose

Expresses the human MAVS CDS containing an N-terminal HA tag and a frameshift mutation inserting TA at bp number 254 of the MAVS CDS . And a GFP marker

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS-Shift

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

A 2 nucleotide insertion was introduced between t…

Promoter

Available since

April 14, 2014

Availability

Academic Institutions and Nonprofits only
pMSCV-GFP

Plasmid

#86537

Purpose

Retrovirus expressing GFP

Depositor

Xue Han

Article

Zhuo et al Elife. 2016 Dec 3;5. pii: e22429. doi: 1

Insert

GFP

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Synapsin I (human)

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pNTI820

Plasmid

#216629

Purpose

cpTurboID (V5) for mammalian expression and lentivirus production

Depositor

Nicholas Ingolia

Article

Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

Insert

cpTurboID

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pNTI661 pRPR1(TetO)-sgRNA

Plasmid

#139475

Purpose

Vector for tet-regulated sgRNA expression in yeast

Depositor

Nicholas Ingolia

Article

McGlincy et al BMC Genomics. 2021 Mar 23;22(1):205. doi

Insert

single guide RNA scaffold

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

pRPR1(TetO)

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pNTI819

Plasmid

#216628

Purpose

Cal-ID (V5) for mammalian expression and lentivirus production

Depositor

Nicholas Ingolia

Article

Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

Insert

Cal-ID

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pScPPX2

Plasmid

#112877

Purpose

Expresses His-tagged yeast exopolyphosphatase for purification from E. coli

Depositor

Ursula Jakob, Michael Gray

Article

Gray et al Mol Cell. 2014 Mar 6;53(5):689-99. doi:

Insert

PPX1 (PPX1 Budding Yeast)

Use

Tags

6X His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pNL4-3 envFS eGFP gag2xFS

Plasmid

#101341

Purpose

1st frameshift is CG nucleotide insertion in MA at nt 832. 2nd is a CTAG addition in CA at nt 1508.

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pNTI757 P(PGK1)-citrine P(HIS4)-mCherry

Plasmid

#164917

Purpose

Divergent promoter expression cassette, P(HIS4) expression with P(PGK1) normalizer

Depositor

Nicholas Ingolia

Article

Muller et al Science. 2020 Dec 11;370(6522). pii: 370

Insert

P(PGK1)-citrine and P(HIS4)-mCherry

Use

Tags

Expression

Yeast

Mutation

Promoter

PGK1 (yeast) and HIS4 (yeast)

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pNTI763 p(Z)-mCherry + UBC9 sgRNA

Plasmid

#164918

Purpose

CEN/ARS with P(Z)-mCherry controlled by the ZEM transcription factor, also encode UBC9 sgRNA

Depositor

Nicholas Ingolia

Article

Muller et al Science. 2020 Dec 11;370(6522). pii: 370

Insert

P(Z)-mcherry

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

P(Z), synthetic promoter

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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