We narrowed to 10,582 results for: plasmids 101

1. pZB260_pT7-STR-H3U3

   Plasmid

   #228506

   Purpose

   Bacterial Hybrid Reporter Plasmid, pT7-HIS3/URA3

   Depositor

   Timothy Whitehead

   Article

   Baumer et al ACS Synth Biol. 2024 Dec 20;13(12):4165-

   Insert

   HIS3/URA3 operon
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available Since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pB2H12

   Plasmid

   #163837

   Purpose

   Two-hybrid system that confers spectinomycin resistance in response to substrate phosphorylation. Constitutive expression of c-src kinase, CDC37, and PTPN12

   Depositor

   Jerome Fox

   Insert

   SpecR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   PTPN12 E57D

   Promoter

   Pro1;ProD;pLacZ-Opt

   Available Since

   July 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. LV2btCRISPRko.v1

   Pooled Library

   #213927

   Purpose

   Uses lentivirus to deliver the library into hard-to-transduce cells

   Depositor

   Wenfang Spring Tan

   Article

   Tan et al PLoS Pathog. 2023 Dec 6;19(12):e1011822.

   Expression

   Mammalian

   Use

   CRISPR and Lentiviral

   Available Since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

4. FUW-TetON-GFP

   Plasmid

   #115495

   Purpose

   Lentiviral plasmid for tet-inducible expression of GFP (generated from plasmid #20321)

   Depositor

   Catherine Verfaillie

   Article

   Garcia-Leon et al Stem Cell Reports. 2018 Feb 13;10(2):655

   Insert

   eGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PBbtCRISPRko.v1

   Pooled Library

   #213928

   Purpose

   Uses the PiggyBac transposon system to deliver the library into hard to transduce cells

   Depositor

   Wenfang Spring Tan

   Article

   Tan et al PLoS Pathog. 2023 Dec 6;19(12):e1011822.

   Expression

   Mammalian

   Use

   CRISPR and Lentiviral

   Available Since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

6. 185_pETcon_SARS2_BQ1-1

   Plasmid

   #208085

   Purpose

   yeast surface display of the SARS-CoV-2 BQ.1.1 variant RBD

   Depositor

   Tyler Starr

   Article

   Taylor et al PLoS Pathog. 2023 Dec 29;19(12):e1011901

   Insert

   SARS-CoV-2 BQ.1.1 RBD (S Budding Yeast, SARS-CoV-2 virus)
   

   Use

   Tags

   HA and c-Myc

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Oct. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Ydf NanoLuc

   Plasmid

   #225656

   Purpose

   Reporter plasmid expressing NanoLuciferase under the control of the promoter PydfJ115, cognate to YdfI. Responds to NarL/NarX chimeras from this study.

   Depositor

   Neha Kamat

   Article

   Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120

   Insert

   NanoLuciferase
   

   Use

   Cell free expression

   Tags

   Myc

   Expression

   Bacterial

   Mutation

   Promoter

   PydfJ115

   Available Since

   Dec. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV-3XFLAG-renilla-luciferase-beta-globin-control

   Plasmid

   #184393

   Purpose

   Transient transfection plasmid expressing Renilla-luciferase-beta-globin fusion protein

   Depositor

   Robert Bradley

   Article

   Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

   Insert

   Renilla luciferase
   

   Use

   Tags

   3X FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AICSDP-078:G3BP1-mEGFP

   Plasmid

   #193920

   Purpose

   Homology arms and mEGFP-SGLRS for N-terminus tagging of human G3BP stress granule assembly factor 1

   Depositor

   Allen Institute for Cell Science

   Insert

   G3BP1 Homology Arms with mEGFP-SGLRS (G3BP1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   mEGFP-linker

   Expression

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available Since

   Dec. 22, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. p663-UBC-miniTurbo-V5-DSTYK _IDG-K

    Plasmid

    #158182

    Purpose

    Gateway destination clone of DSTYK (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-DSTYK (DSTYK Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available Since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. SUMO CRYGS 16-177 Q16C

    Plasmid

    #217673

    Purpose

    N-terminally truncated human gamma S crystallin residues 16-177 with Q16C mutation for native chemical ligation

    Depositor

    Larry David

    Insert

    CRYGS (CRYGS Human)
    

    Use

    Tags

    6XHis and SUMO

    Expression

    Bacterial

    Mutation

    Glutamine 16 changed to Cysteine, and amino acids…

    Promoter

    T7

    Available Since

    May 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p667-UBC-HIPK3-V5-miniTurbo_IDG-K

    Plasmid

    #158191

    Purpose

    Gateway destination clone of HIPK3 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    HIPK3-V5-miniTurbo (HIPK3 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available Since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. 186_pETcon_SARS2_XBB1-5

    Plasmid

    #208086

    Purpose

    yeast surface display of the SARS-CoV-2 XBB.1.5 variant RBD

    Depositor

    Tyler Starr

    Article

    Taylor et al PLoS Pathog. 2023 Dec 29;19(12):e1011901

    Insert

    SARS-CoV-2 XBB.1.5 RBD (S Budding Yeast, SARS-CoV-2 virus)
    

    Use

    Tags

    HA and c-Myc

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. modRNAc0-Cas9-2A-Puro

    Plasmid

    #172855

    Purpose

    IVT template plasmid for making modified Cas9Puro RNA

    Depositor

    Xiaojun Lian

    Article

    Haideri et al Cell Rep Methods. 2022 Sep 7;2(9):100290

    Insert

    Cas9-2A-Puro
    

    Use

    Ivt template plasmid

    Tags

    2A-Puro

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pM2s2AsR_TS-E10

    Plasmid

    #137944

    Purpose

    E. coli Nissle pMUT2-derived plasmid with ampicillin resistance and constitutive RFP expression and strong temperature sensitive GFP expression

    Depositor

    Neel Joshi

    Article

    Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102

    Inserts

    mRFP1
    mWasabi
    

    Use

    Synthetic Biology; For use with e. coli nissle in…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23101 and pTlpA-C7

    Available Since

    May 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFOXA3-donor

    Plasmid

    #112321

    Purpose

    CRISPR donor plasmid to tag human transcription factor FOXA3 with GFP

    Depositor

    Kevin White

    Insert

    FOXA3 homology arms flanking EGFP-IRES-Neo cassette (FOXA3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    hg38:19:45872401:G>T, hg38:19:45872421:C>T,…

    Promoter

    Available Since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pENN.AAV.CB7.CI.CNDP1-flag.WPRE.rBG

    Plasmid

    #132683

    Purpose

    AAV plasmid encoding mouse CNDP1 with C-terminal flag tag

    Depositor

    Jonathan Long

    Article

    Kim et al Cell Chem Biol. 2019 Nov 21;26(11):1623-

    Insert

    Carnosine dipeptidase 1 (Cndp1 Mouse)
    

    Use

    AAV

    Tags

    Flag

    Expression

    Mutation

    Promoter

    Chicken b-actin

    Available Since

    Nov. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSN16

    Plasmid

    #100103

    Purpose

    expresses a chimeric OCT4-activator fusion

    Depositor

    Mark Ptashne

    Article

    Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

    Insert

    vexGFP-P2A-OCT4 fused to a 'strong' VP16 domain (POU5F1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSN20

    Plasmid

    #100105

    Purpose

    expresses a chimeric OCT4-activator fusion

    Depositor

    Mark Ptashne

    Article

    Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

    Insert

    vexGFP-P2A-OCT4 fused to a 'medium' VP16 domain (POU5F1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSN21

    Plasmid

    #100106

    Purpose

    expresses a chimeric OCT4-activator fusion

    Depositor

    Mark Ptashne

    Article

    Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

    Insert

    vexGFP-P2A-OCT4 fused to a 'weak' VP16 domain (POU5F1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge