We narrowed to 4,163 results for: spl

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pTrcHisB-TRF1

Plasmid

#53209

Purpose

Expressed N-terminally Hexahistidine tagged TRF1 protein

Depositor

Jack Griffith

Article

Bower et al Biochemistry. 2014 Sep 2;53(34):5485-95.

Insert

TRF1 (TERF1 Human)

Tags

Hexahistidine

Expression

Bacterial

Mutation

3-439

Promoter

Trc

Available Since

May 20, 2014

Availability

Academic Institutions and Nonprofits only

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LgBiT-hACE2

Plasmid

#173431

Purpose

protein expression plasmid of LgBiT-hACE2-IgG1 Fc

Depositor

David Nemazee

Article

Huang et al J Immunol. 2021 Jul 1;207(1):344-351. do

Insert

LgBiT-hACE2-IgG1 Fc (ACE2 Human)

Use

Luciferase

Tags

IgG1

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2+HA-hSeparase

Plasmid

#33018

Depositor

Marc Kirschner

Article

Stemmann et al Cell. 2001 Dec 14;107(6):715-26.

Insert

Separase (ESPL1 Human)

Tags

3xHA

Expression

Mammalian

Promoter

simian CMV IE94

Available Since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only
Myc-CYLD-C601A

Plasmid

#60027

Purpose

Myc-tagged CYLD catalytic inactive (C601A) expression

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

Insert

Cylindromatosis (CYLD Human)

Tags

Myc

Expression

Mammalian

Mutation

Cysteine 601 changed to Alanine

Promoter

CMV

Available Since

Nov. 25, 2014

Availability

Academic Institutions and Nonprofits only

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pTR-UF11 (AAV.A12.T)

Viral Prep

#157970-AAV.A12.T

Purpose

Ready-to-use AAV44.9(E531D) in PBS particles produced from pTR-UF11 (#157970). In addition to the viral particles, you will also receive purified pTR-UF11 plasmid DNA. 'Humanized' GFP driven by Chimeric CMV/Chicken Beta Actin promoter

Depositor

Sergei Zolotukhin

Article

Burger et al Mol Ther. 2004 Aug;10(2):302-17. doi: 10

Promoter

chimeric CMV/Chicken Beta actin (CBA)

Tags

GFP

Available Since

Dec. 21, 2020

Availability

Academic Institutions and Nonprofits only
TDP-REGv2(mScarlet-FLAG)#10 in pTwist-CMV backbone

Plasmid

#216154

Purpose

Expresses mScarlet in cells with TDP-43 loss-of-function. Leaky but very sensitive to mild TDP-43 loss of function. It uses a cryptic exon. [Code 'B5'] Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

Depositor

Pietro Fratta

Article

Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

Insert

mScarlet with cryptic exon and C-terminal FLAG

Expression

Mammalian

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-CYLD-STOP

Plasmid

#60077

Purpose

N-terminal GFP tagged CYLD with stop codon, driven by CMV promoter

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

Insert

Cylindromatosis (CYLD Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

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TDP-REGv2(mScarlet-FLAG) matched positive control in pTwist-CMV

Plasmid

#216156

Purpose

Expresses mScarlet regardless of TDP-43 knockdown. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

Depositor

Pietro Fratta

Article

Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

Insert

mScarlet with C-terminal FLAG tag

Expression

Mammalian

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pATLIC

Plasmid

#110287

Purpose

A backbone vector for cell surface display in E. coli by Ligation Independent Cloning (LIC)

Depositor

In-Geol Choi

Article

Ko et al Appl Environ Microbiol. 2012 May;78(9):3

Insert

yfaL

Expression

Bacterial

Promoter

araBAD promoter

Available Since

April 22, 2019

Availability

Academic Institutions and Nonprofits only

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GST-PRMT9 WT

Plasmid

#67607

Purpose

bacterial expression of wild type human PRMT9

Depositor

Steven Clarke

Article

Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

Insert

PRMT9 (PRMT9 Human)

Tags

GST

Expression

Bacterial

Mutation

wild type

Promoter

tac

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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FR_HNF4A8

Plasmid

#31114

Depositor

Gerhart Ryffel

Article

Erdmann et al Biol Chem. 2007 Jan . 388(1):91-106.

Insert

HNF4A (HNF4A Human)

Tags

Myc

Expression

Mammalian

Mutation

splice variant 8

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pCR3.1-OPNc

Plasmid

#107982

Purpose

expression of OPN splice variant c

Depositor

Georg Weber

Article

He et al Oncogene. 2006 Apr 6;25(15):2192-202. do

Insert

osteopontin splice variant c (SPP1 Human)

Expression

Mammalian

Mutation

splice variant

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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pCR3.1-OPNb

Plasmid

#107981

Purpose

expression of OPN splice variant b

Depositor

Georg Weber

Article

He et al Oncogene. 2006 Apr 6;25(15):2192-202. do

Insert

osteopontin splice variant b (SPP1 Human)

Expression

Mammalian

Mutation

splice variant

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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FLAG SMG1 WT

Plasmid

#199573

Purpose

Expresses FLAG-tagged WT SMG1

Depositor

Michael Kastan

Article

McCann et al Radiat Res. 2023 Apr 1;199(4):406-421. d

Insert

SMG1 (SMG1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV5M/rDlg1

Plasmid

#28259

Depositor

Jeffrey Frost

Article

Carr et al J Biol Chem. 2009 Sep 4. 284(36):24269-8

Insert

Dlg1 I3

Tags

Myc

Expression

Mammalian

Available Since

April 14, 2011

Availability

Academic Institutions and Nonprofits only
rNrx1beta(47-299)

Plasmid

#20173

Depositor

Axel Brunger

Article

Arac et al Neuron. 2007 Dec 20. 56(6):992-1003.

Insert

Neurexin 1 beta (Nrxn1 Rat)

Tags

6X His tag

Expression

Insect

Mutation

no splice site

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
Cav2.2e[Δa10, Δ18a, Δ24a, 31a, 37a, 46]

Plasmid

#26569

Depositor

Diane Lipscombe

Article

Bell et al Neuron. 2004 Jan 8. 41(1):127-38.

Insert

calcium channel alpha-1B subunit (Cacna1b Rat)

Tags

6XHis and V5

Expression

Mammalian

Mutation

Deletion of exons a10, 18a, and 24a. Contains …

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only
YFP-Rac2(N17)

Plasmid

#11398

Depositor

Joel Swanson

Article

Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

Insert

ras-related C3 botulinum toxin substrate 2 (RAC2 Human)

Tags

YFP

Expression

Mammalian

Mutation

Obtained with Threonine 17 changed to Asparagine.

Available Since

Feb. 21, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_FLAG-YAP1

Plasmid

#173001

Purpose

constitutive expression of FLAG-tagged YAP1

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

FLAG-YAP1 (YAP1 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP_sfCherry2(11)_HP1

Plasmid

#82607

Purpose

Expresses sfCherry2(11) tagged heterochromatin protein1 in mammalian cells

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

sfCherry2(11)_HP1b

Expression

Mammalian

Mutation

changed Gly12 to Ala in sfCherry11+

Available Since

June 29, 2017

Availability

Academic Institutions and Nonprofits only