We narrowed to 59,245 results for: {{{s}}}

1. psgRNA-GST-EGFP-GPIBY55

   Plasmid

   #213720

   Purpose

   To express a single-guide RNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPIBY55 under a CMV promoter.

   Depositor

   Qilai Huang

   Article

   Ma et al Front Mol Biosci. 2022 Sep 29;9:1016090.

   Insert

   EGFP
   

   Use

   CRISPR

   Tags

   GST

   Expression

   Mutation

   Promoter

   CMV

   Available since

   April 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. psgRNA-GST-EGFP-GPIDAF

   Plasmid

   #213719

   Purpose

   To express a single-guide RNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPIDAF under a CMV promoter.

   Depositor

   Qilai Huang

   Article

   Ma et al Front Mol Biosci. 2022 Sep 29;9:1016090.

   Insert

   EGFP
   

   Use

   CRISPR

   Tags

   GST

   Expression

   Mutation

   Promoter

   CMV

   Available since

   April 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. psgRNA-GST-EGFP-GPICEAM7

   Plasmid

   #213721

   Purpose

   To express a single-guide RNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPICEAM7 under a CMV promoter.

   Depositor

   Qilai Huang

   Article

   Ma et al Front Mol Biosci. 2022 Sep 29;9:1016090.

   Insert

   EGFP
   

   Use

   CRISPR

   Tags

   GST

   Expression

   Mutation

   Promoter

   CMV

   Available since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHyg-His6

   Plasmid

   #212776

   Purpose

   C-terminal PCR-based tagging with His6 in budding yeast with HygR selection

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Type

   Empty backbone

   Use

   Pcr-based tagging

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. TRAPa(R150A)-3xFLAG-pCDNA

   Plasmid

   #201966

   Purpose

   Mammalian expression of mutated Translocon-associated protein subunit alpha

   Depositor

   Ville Paavilainen

   Article

   Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

   Insert

   SSR1 (SSR1 Human)
   

   Use

   Tags

   3xFLAG-tag

   Expression

   Mammalian

   Mutation

   R150A

   Promoter

   Available since

   Jan. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pETM11-6xHis-TEV-hORF1p_StammerAAA

   Plasmid

   #202587

   Purpose

   Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the M91A, E92A, and L93A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification

   Depositor

   Liam Holt

   Article

   Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

   Insert

   ORF1p
   

   Use

   Tags

   6x His and TEV protease cleavage site

   Expression

   Bacterial

   Mutation

   Changed ORF1p Methionine 91 to Alanine, ORF1p Glu…

   Promoter

   Available since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pETM11-6xHis-TEV-hORF1p_StammerAEA

   Plasmid

   #202588

   Purpose

   Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the M91A and L93A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification

   Depositor

   Liam Holt

   Article

   Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

   Insert

   ORF1p
   

   Use

   Tags

   6x His and TEV protease cleavage site

   Expression

   Bacterial

   Mutation

   Changed ORF1p Methionine 91 to Alanine, changed O…

   Promoter

   Available since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET-28b(+)/ADK C77S

   Plasmid

   #201409

   Purpose

   Overexpresses Escherichia coli adenylate kinase C77S

   Depositor

   Gary Pielak

   Article

   Brom et al Biomol NMR Assign. 2023 Aug 26. doi: 10.

   Insert

   Escherichia coli adenylate kinase C77S (adk Escherichia coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changed cysteine 77 to serine

   Promoter

   Available since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. NbV5-TEV219S153N

   Plasmid

   #201488

   Purpose

   TANGO detection of V5-tagged protein

   Depositor

   Patrick Giguère

   Article

   Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

   Insert

   NbV5-TEV219S153N
   

   Use

   Tags

   TEV219 S153N

   Expression

   Mammalian

   Mutation

   S153N in TEV219

   Promoter

   CMV

   Available since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pminiTol2-4kb wnt3 promoter-PMTmApple

    Plasmid

    #203794

    Purpose

    Tol2 based genomic integration of zebrafish 4kb Wnt3 promoter driving the expression of PMTmApple for zebrafish transgenesis

    Depositor

    Thorsten Wohland

    Article

    Veerapathiran et al Elife. 2020 Nov 25;9:e59489. doi: 10.755

    Insert

    PMT
    

    Use

    Zebrafish expression

    Tags

    mApple

    Expression

    Mutation

    Promoter

    4kb Wnt3 promoter

    Available since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBS-TRE-mRuby3-WPRE

    Plasmid

    #193336

    Purpose

    Expresses mRuby3 under the TRE promoter in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

    Insert

    mRuby3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBS-TRE-YPet-WPRE

    Plasmid

    #193335

    Purpose

    Expresses YPet under the TRE promoter in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

    Insert

    YPet
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBS-TRE-mAmetrine1.1-WPRE

    Plasmid

    #193333

    Purpose

    Expresses mAmetrine1.1 under the TRE promoter in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

    Insert

    mAmetrine1.1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBS-TRE-mNeonGreen-WPRE

    Plasmid

    #193334

    Purpose

    Expresses mNeonGreen under the TRE promoter in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBad-HaloGFP-145-146

    Plasmid

    #198600

    Purpose

    Green fluorescent Ca2+ ion indicator for bacterial expression.

    Depositor

    Robert Campbell

    Article

    Zhu et al Nat Chem Biol. 2023 Jan;19(1):38-44. doi

    Insert

    HaloGFP-145-146
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD promoter

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBad-HaloGFP-169-170

    Plasmid

    #198612

    Purpose

    Green fluorescent Ca2+ ion indicator for bacterial expression.

    Depositor

    Robert Campbell

    Article

    Zhu et al Nat Chem Biol. 2023 Jan;19(1):38-44. doi

    Insert

    HaloGFP-169-170
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD promoter

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBAD-P19-EGFP

    Plasmid

    #196609

    Purpose

    siRNA vector expressing TMV P19 and a 720-long EGFP sequence as inverted repeat

    Depositor

    Franco Lucchini

    Article

    Villa et al Virology. 2022 Dec 31;579:38-45. doi: 10

    Insert

    p19 + EGFP inverted repeat sequence
    

    Use

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    AraC

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBad-HaloGFP-Ca0.5

    Plasmid

    #198632

    Purpose

    Green fluorescent Ca2+ ion indicator for bacterial expression.

    Depositor

    Robert Campbell

    Article

    Zhu et al Nat Chem Biol. 2023 Jan;19(1):38-44. doi

    Insert

    HaloGFP-Ca0.5
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD promoter

    Available since

    April 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBad-HaloGFP-Ca0.2

    Plasmid

    #198631

    Purpose

    Green fluorescent Ca2+ ion indicator for bacterial expression.

    Depositor

    Robert Campbell

    Article

    Zhu et al Nat Chem Biol. 2023 Jan;19(1):38-44. doi

    Insert

    HaloGFP-Ca0.2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD promoter

    Available since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBad-HaloGFP-Ca1

    Plasmid

    #198633

    Purpose

    Green fluorescent Ca2+ ion indicator for bacterial expression.

    Depositor

    Robert Campbell

    Article

    Zhu et al Nat Chem Biol. 2023 Jan;19(1):38-44. doi

    Insert

    HaloGFP-Ca1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD promoter

    Available since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge