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pcDNA3-EKAR4

Plasmid

#174437

Purpose

Improved cyan/yellow FRET-based ERK kinase activity reporter.

Depositor

Jin Zhang

Article

Keyes et al eLife. 2020 May 26;9. pii: 57410. doi: 1

Insert

EKAR4

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pOPINB-AMSH*

Plasmid

#66712

Purpose

Expresses activated AMSH in E. coli

Depositor

David Komander

Article

Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

Inserts

hbp (Stam2 Mouse)
AMSH (STAMBP Human)

Tags

His-3C

Expression

Bacterial

Promoter

T7

Available Since

July 17, 2015

Availability

Academic Institutions and Nonprofits only
HA-hNM2B

Plasmid

#236533

Purpose

For expression of NM2B (human) in mammalian cells

Depositor

Sandra Citi

Article

Rouaud et al J Cell Biol. 2023 Jul 3;222(7):e20220806

Insert

MYH10 (MYH10 Human)

Tags

HA

Expression

Mammalian

Mutation

WT

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag Jnk1a2

Plasmid

#13751

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk1a2 (MAPK8 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pMetYCgate

Plasmid

#105074

Purpose

Yeast mating-based Split Ubiquitin Assay, bait vector for in vivo cloning

Depositor

Wolf Frommer

Article

Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

Type

Empty backbone

Tags

Cub-PLV

Expression

Yeast

Available Since

Feb. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pEGB 35S:Renilla:Tnos (GB0109)

Plasmid

#68215

Purpose

Transcriptional unit for Renilla Luciferase Protein plant expression driven by the 35S promoter

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Renilla

Use

Luciferase and Synthetic Biology

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

35S

Available Since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only

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pMx/ATP8B3-HA

Plasmid

#209228

Purpose

Mammalian expression of ATP8B3

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

Insert

ATP8B3 (ATP8B3 Human)

Use

Retroviral

Tags

HA

Available Since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pAC-mCherry2-G4

Plasmid

#164167

Purpose

Expresses mCherry2 under the control of CMV promoter, basic vector for AAV, pAAV-CMV backbone, multiclonig site at C terminal

Depositor

Isei Tanida

Article

Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

Insert

mCherry2

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pNXgate33-3HA

Plasmid

#105075

Purpose

Yeast mating-based Split Ubiquitin Assay, prey vector for in vivo cloning

Depositor

Wolf Frommer

Article

Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

Type

Empty backbone

Tags

3xHA and NubG

Expression

Yeast

Available Since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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siRNA-resistant pLV-SigmaR1-mNeonGreen

Plasmid

#226569

Purpose

Encodes siRNA resistant SigmaR1 protein labeled with mNeonGreen (lentiviral vector)

Depositor

Gia Voeltz

Article

Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

Insert

SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)

Use

Lentiviral

Tags

mNeonGreen

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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RP125 pUAST EGFP:Orco

Plasmid

#53441

Purpose

N-terminal fusion of EGFP to Drosophila melanogaster Orco

Depositor

Leslie Vosshall

Article

Benton et al PLoS Biol. 2006 Feb;4(2):e20. Epub 2006

Insert

EGFP:Orco (Orco Fly)

Tags

EGFP

Expression

Insect

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only
pS1643 (pFA6-NATMX-CDC42pr-yGFP1-10)

Plasmid

#86420

Purpose

N-terminal PCR tagging cassette for yeast codon optimized split GFP1-10 with CDC42 promoter

Depositor

Sue Jaspersen

Article

Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

Insert

CDC42pr-GFP1-10

Tags

GFP1-10

Expression

Yeast

Available Since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pmCherry-Akt1(WT)

Plasmid

#86631

Purpose

Expresses mCherry-tagged human Akt1(WT)

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1 (AKT1 Human)

Expression

Mammalian

Available Since

April 4, 2017

Availability

Academic Institutions and Nonprofits only

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pET19b-EcMscL-TEV-mEGFP

Plasmid

#165097

Purpose

Expresses EcMscLGFP as a fusion protein in E. coli BL21or using in vitro expression.

Depositor

Neha Kamat

Article

Jacobs et al Proc Natl Acad Sci U S A. 2019 Feb 13. p

Insert

E. Coli Mechanosensitive Channel of Large Conductance (mscL E. coli)

Use

Synthetic Biology

Tags

monomeric Enhanced Green Fluorescent Protein

Expression

Bacterial

Promoter

T7

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNAKv10.1mVenus

Plasmid

#85705

Purpose

Kv10.1 fused mVenus

Depositor

Luis Pardo

Article

Chen et al PLoS One. 2011 May 3;6(5):e19257. doi: 1

Insert

KCNH1 (KCNH1 Human)

Tags

mVenus

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pPBpuro-miRFP670-HRasCT

Plasmid

#176030

Purpose

Encoding membrane-targeted miRFP670

Depositor

Michiyuki Matsuda

Article

Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

Insert

Membrane-targeted miRFP670

Expression

Mammalian

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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SNAP-Dcp2

Plasmid

#153975

Purpose

SNAP-tag polypeptide fused to mRNA decapping enzyme 2

Depositor

Gia Voeltz

Article

Lee et al Science. 2020 Jan 31;367(6477). pii: 367

Insert

mRNA Decapping enzyme 2 (DCP2 Human)

Tags

SNAP-C1

Expression

Mammalian

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pBITE-EGFRvIII-F263-OKT3

Plasmid

#190680

Purpose

Encodes an EGFRvIII-CD3 BITE protein (F263 clone)

Depositor

Scott McComb

Article

Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

Insert

Anti-EGFRvIII-scFv-F263-modularized-OKT3-HisTag

Use

Lentiviral

Tags

mNeonGreen

Expression

Mammalian

Promoter

EFS

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-Tight-Puro_MYRF

Plasmid

#64843

Purpose

A lentiviral vector for the expression of mouse MYRF under doxycyline control.

Depositor

Paul Tesar

Article

Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

Insert

MYRF (Myrf Mouse)

Use

Lentiviral

Expression

Bacterial and Mammalian

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only

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pOPINB-OTUB1*

Plasmid

#65441

Purpose

Expresses activated OTUB1 in E. coli

Depositor

David Komander

Article

Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

Inserts

UbcH5b (UBE2D2 Human)
otubain-1 (OTUB1 )

Tags

His-3C

Expression

Bacterial

Mutation

Active site cysteine mutated to alanine

Promoter

T7

Available Since

July 17, 2015

Availability

Academic Institutions and Nonprofits only