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FER

Plasmid

#187418

Purpose

Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

Depositor

Susanne Dobler

Article

Mohammadi et al PLoS Genet. 2022 Aug 16;18(8):e1010323.

Inserts

ATP1A1
ATP1B1

Expression

Insect

Promoter

PH and p10

Available Since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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CHI

Plasmid

#187417

Purpose

Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

Depositor

Susanne Dobler

Article

Mohammadi et al PLoS Genet. 2022 Aug 16;18(8):e1010323.

Inserts

ATP1A1
ATP1B1

Expression

Insect

Promoter

PH and p10

Available Since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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MBP_Lims (MBP_LIM1234)

Plasmid

#182042

Purpose

For expression of human paxillin LIM domains 1-4 with N-terminal GST fusion protein in E. coli.

Depositor

Vesa Hytönen

Article

Ripamonti et al Commun Biol. 2021 Mar 29;4(1):380. doi:

Insert

LIM1234 (PXN Human)

Tags

MBP

Expression

Bacterial

Mutation

LIM domains 1-4 only

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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GST_LIM234

Plasmid

#182039

Purpose

For expression of human paxillin LIM domains 2-4 with N-terminal GST fusion protein in E. coli.

Depositor

Vesa Hytönen

Article

Ripamonti et al Commun Biol. 2021 Mar 29;4(1):380. doi:

Insert

LIM234 (PXN Human)

Tags

GST

Expression

Bacterial

Mutation

LIM domains 2-4 only

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-RiboJ-mTFP1-T7

Plasmid

#173225

Purpose

Encodes mTFP fluorescent protein. To be used in cell-free system

Depositor

Jim Haseloff

Article

Guzman-Chavez et al ACS Synth Biol. 2022 Mar 18;11(3):1114-1

Insert

mTFP1

Use

Cell-free system

Expression

Bacterial

Promoter

T7

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pFGC-T7RibJ-RRvT

Plasmid

#173226

Purpose

Encodes RRvT fluorescent protein. To be used in cell-free system

Depositor

Jim Haseloff

Article

Guzman-Chavez et al ACS Synth Biol. 2022 Mar 18;11(3):1114-1

Insert

RRvT

Use

Cell-free system

Expression

Bacterial

Promoter

T7

Available Since

March 9, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pDSARN-vasa-eSpCas9sv40

Plasmid

#173670

Purpose

eSpCas9 expression vector for Anopheles

Depositor

Eric Marois

Article

Klug et al PLoS Pathog. 2022 Oct 12;18(10):e1010881

Insert

eSpCas9 with Anopheles vasa promoter and SV40 terminator

Use

CRISPR

Expression

Insect

Mutation

mutations in Cas9 reducing off-target activity (S…

Promoter

vasa

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Flag-stm2585

Plasmid

#174391

Purpose

S. Typhimurium gene stm2585 (sarA/steE) codon-optimized for mammalian expression with N-terminal Flag tag

Depositor

Dennis Ko

Article

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

stm2585

Tags

Flag

Expression

Mammalian

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-GFAP104-ChromeT-mCherry

Plasmid

#123126

Purpose

AAV-mediated expression of ChromeT-mCherry under the GFAP promoter.

Depositor

Edward Boyden

Article

Cho et al J Biol Chem. 2019 Jan 4. pii: RA118.0069

Insert

ChromeT-mCherry

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

Channelrhodopsin-2 (ChR2) with mutations A71S/ E9…

Promoter

GFAP104

Available Since

April 1, 2021

Availability

Academic Institutions and Nonprofits only

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pCFJ1972 - [1-2] ENTR - Flour - TagBFP2(NLS(2), no_atg, no_stop)

Plasmid

#159844

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Flour - TagBFP2(NLS(2), no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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