We narrowed to 31,814 results for: LIS;

1. pIG-601_NY-ESO-1_TCR_GFP

   Plasmid

   #207482

   Purpose

   This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   GFP, NY-ESO-1_TCR
   

   Use

   CRISPR

   Mutation

   Potential silent point mutation in GFP sequence

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMV-SB100X[EF1α-copGFP]

   Plasmid

   #218323

   Purpose

   Donor plasmid of SB100X Transposon with copGFP mammalian expression cassette

   Depositor

   Haoyi Wang

   Article

   Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

   Insert

   copGFP mammalian expression cassette
   

   Expression

   Mammalian

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGP-AAV-syn-FLEX-jGCaMP8m-WPRE

   Plasmid

   #162378

   Purpose

   AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expression

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Has Service

   AAV Retrograde, AAV1, AAV5, and AAV9

   Insert

   jGCaMP8m
   

   Use

   AAV and Cre/Lox

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   A25G F286Y

   Promoter

   Synapsin

   Available Since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA-HA-ER Y537S

   Plasmid

   #49499

   Purpose

   Expresses HA-tagged ER Y537S mutant in mammalian cells

   Depositor

   Sarat Chandarlapaty

   Article

   Toy et al Nat Genet. 2013 Dec;45(12):1439-45. doi:

   Insert

   ESR1 (ESR1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Tyrosine 537 to Serine

   Promoter

   CMV

   Available Since

   Dec. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX037

   Plasmid

   #167156

   Purpose

   MoClo-compatible Level2 vector for autonomous bioluminescence in plants encoding kanamycin resistance cassette, nnHispS, nnH3H, nnLuz and nnCPH. Does not contain NpgA.

   Depositor

   Karen Sarkisyan

   Article

   Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

   Insert

   caffeic acid cycle genes
   

   Expression

   Plant

   Promoter

   pNOS, p35s

   Available Since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA6-N-3XFLAG-Ctnnb1

   Plasmid

   #123586

   Depositor

   Bruce Beutler

   Article

   Choi et al Science. 2019 May 10;364(6440). pii: 364

   Insert

   catenin (cadherin associated protein), beta 1 (Ctnnb1 Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. TP53-KO_gRNA_1

   Plasmid

   #195130

   Purpose

   TP53-targeting gRNA in the LRG2.1 backbone

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   TP53 KO gRNA 1 (TP53 Human)
   

   Expression

   Mammalian

   Available Since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-mTurquoise2-Caspase-1-mNeonGreen

   Plasmid

   #166784

   Purpose

   FRET analysis (pro caspase-1 cleavage) in living cells

   Depositor

   Hao Wu

   Article

   Magupalli et al Science. 2020 Sep 18;369(6510). pii: 369

   Insert

   mTurquoise2-caspase-1-mNeonGreen (CASP1 Human)
   

   Use

   Lentiviral

   Tags

   mNeonGreen and mTurquoise2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDF_AtRaf1/Raf2/RbcX2/BSD2/RbcX1

   Plasmid

   #229510

   Purpose

   Expresses Arabidopsis thaliana chaperones: Raf1,Raf2,RbcX2,BSD2,RbcX1

   Depositor

   Laura Gunn

   Article

   Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849.

   Inserts

   Rubisco accumulation factor 1 (AT3G04550 Mustard Weed)
   Rubisco accumulation factor 2 (AT5G51110 Mustard Weed)
   RbcX2 (RbcX2 Mustard Weed)
   BSD2
   RbcX1
   

   Expression

   Bacterial

   Mutation

   N terminus chloroplast transit peptide truncation

   Promoter

   T7

   Available Since

   June 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1 SIRT7_H187Y

    Plasmid

    #53151

    Purpose

    expression vector for catalytically inactive human Sirtuin7

    Depositor

    Katrin Chua

    Article

    Shin et al Cell Rep. 2013 Nov 14;5(3):654-65. doi:

    Insert

    Sirt7 (SIRT7 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    H187Y, catalytic inactive

    Promoter

    CMV

    Available Since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV-mTurquoise2-IL-1beta-mNeonGreen

    Plasmid

    #166783

    Purpose

    FRET analysis (pro-IL-1beta cleavage) in living cells

    Depositor

    Hao Wu

    Article

    Magupalli et al Science. 2020 Sep 18;369(6510). pii: 369

    Insert

    mTurquoise2-IL-1b-mNeongreen (IL1B Human)
    

    Use

    Lentiviral

    Tags

    mNeonGreen and mTurquoise2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. PE7_AAVS1_knockin_HDR_donor

    Plasmid

    #223001

    Purpose

    HDR donor plasmid for PE7 knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PE7-IRES2-EGFP-WPRE-βglobinpA-right_homology_arm

    Depositor

    Brittany Adamson

    Article

    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

    Insert

    PE7
    

    Use

    Hdr donor

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mammalian

    Promoter

    EF1α

    Available Since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. TIGRE docking site targeting vector

    Plasmid

    #61580

    Purpose

    Create a docking site in the mouse TIGRE genomic locus that allows modification in ES cells by Flp recombinase-mediated cassette exchange

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Type

    Empty backbone

    Use

    Mouse Targeting

    Available Since

    July 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGL3-sgRab18.N-Cas9-T2A-mCherry-P2A-Puro

    Plasmid

    #129418

    Purpose

    Encoding Cas9 and sgRAB18.N for CRISPR/Cas9 mediated HDR tagging of endogenous human RAB18 N-terminus

    Depositor

    Elina Ikonen

    Article

    Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

    Insert

    SpCas9 and sgRAB18.N (RAB18 Human)
    

    Use

    CRISPR

    Tags

    T2A-mCherry-P2A-Puro

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-pCALM1-sFLEx-HA-SpCas9-miniU6-sgRNAShank3

    Plasmid

    #213969

    Purpose

    AAV vector for encoding SpCas9 driven by pCALM1 promoter targeting Shank3 locus in the presence of Cre recombinase

    Depositor

    Zhonghua Lu

    Article

    Wang et al Cell Rep. 2023 Nov 28;42(11):113348. doi

    Insert

    Shank3 sgRNA
    

    Use

    AAV and CRISPR

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA6-N-3XFLAG-Gsk3b

    Plasmid

    #123592

    Depositor

    Bruce Beutler

    Article

    Choi et al Science. 2019 May 10;364(6440). pii: 364

    Insert

    glycogen synthase kinase 3 beta (Gsk3b Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDH-EF1-IRES-BSD

    Plasmid

    #209751

    Purpose

    Lentiviral transfer plasmid to express the blasticidin resistance gene

    Depositor

    Andrei Thomas-Tikhonenko

    Article

    Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. dCas9-GCN5 core

    Plasmid

    #179551

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human GCN5 core (aa 473-676) driven by EF-1alpha promoter

    Depositor

    Isaac Hilton

    Article

    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

    Insert

    S. pyogenes dCas9 with c-terminal fusion of human GCN5 core (aa 473-676) (KAT2A Human)
    

    Use

    Lentiviral

    Tags

    Flag Tag

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    EF-1alpha

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. shTCF7L2 # 1

    Plasmid

    #42557

    Depositor

    William Hahn

    Article

    Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

    Insert

    shTCF7L2 (TCF7L2 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Feb. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

20. lentiCRISPR v2-sgSLC7A11/xCT-2

    Plasmid

    #161819

    Purpose

    knock out SLC7A11/xCT in mammalian cells

    Depositor

    Boyi Gan

    Article

    Liu et al Nat Cell Biol. 2020 Apr;22(4):476-486. d

    Insert

    solute carrier family 7 member 11 (SLC7A11 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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