We narrowed to 11,025 results for: 158

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mEos3.2trCD4o

Plasmid

#203751

Purpose

Encodes the transmembrane domain from CD4, including its 2 palmitoylation sites, with mEos3.2 fused on the N terminus. Used as a fluorescent plasma membrane probe.

Depositor

Sarah Veatch

Article

Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

Insert

trCD4o (CD4 Human)

Tags

mEos3.2

Expression

Mammalian

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pLX304_zeo_eGFP_V5

Plasmid

#160096

Purpose

Expresses eGFP in mammalian cells

Depositor

Rizwan Haq

Article

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Insert

eGFP

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pLX307 ARHGAP30

Plasmid

#117738

Purpose

Open reading frame vector encoding ARHGAP30

Depositor

William Hahn

Article

Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

Insert

ARHGAP30 (ARHGAP30 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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A*01:03:01

Plasmid

#203293

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*01:03:01 (HLA-A Human)

Expression

Mammalian

Available Since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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WT N-His Ana GvpC in pET28a

Plasmid

#153294

Purpose

Expresses N-terminal His-tagged GvpC from Anabaena flos-aquae in E.coli

Depositor

Mikhail Shapiro

Article

Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

Insert

Gas vesicle protein C (N-terminal his-tagged)

Tags

His-tag

Expression

Bacterial

Mutation

Added a hexahistidine tag after the methionine at…

Promoter

T7

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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HA-BAP1-S172R-GFP

Plasmid

#78918

Purpose

Mammalian expression of BAP1 with S172R and an EGFP fusion

Depositor

Michael Hendzel

Article

Ismail et al Cancer Res. 2014 Aug 15;74(16):4282-94.

Insert

BAP1 (BAP1 Human)

Tags

EGFP and HA

Expression

Mammalian

Mutation

S172R

Promoter

CMV

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
pCMV-NES-TvmvC-43LK-iLID-TvmvN-TVMVseq(P1'S)-mCherry

Plasmid

#210504

Purpose

expresses NES-TvmvC-43LK-iLID-TvmvN-TVMVseq(P1'S)-mCherry component in mammalian cells

Depositor

Dongmin Lee

Article

Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

Insert

NES-TvmvC-43LK-iLID-TvmvN-TVMVseq(P1'S)-mCherry

Expression

Mammalian

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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Polymerase expression - pCMV-B103 (LM2907)

Plasmid

#208968

Purpose

Unfused B103 DNA polymerase, expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

B103-BPNLS

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Promoter

CMV and T7

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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nDHFR173-gs10-NLS-VPR

Plasmid

#188262

Purpose

Plasmid ensures constitutive expression of the N-terminal fragment of split DHFR (aa 174-186), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus

Depositor

Roman Jerala

Article

Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

Insert

nDHFR173 (DHFR Human)

Expression

Mammalian

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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FH1-PNGT#1-mCherry

Plasmid

#164098

Purpose

Fluorescent reporter for genetic tracing of proneural Glioblastoma cell-state.

Depositor

Gaetano Gargiulo

Article

Schmitt et al Cancer Discov. 2021 Mar;11(3):754-777. d

Insert

PNGT#1-mCherry

Use

Lentiviral and Synthetic Biology

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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mEos3.2trLAT-2P

Plasmid

#203748

Purpose

Encodes the transmembrane domain from mouse LAT with mEos3.2 fused on the N terminus. Palmitoylation sites at C27 and C30 mutated to A. Used as a fluorescent plasma membrane probe.

Depositor

Sarah Veatch

Article

Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

Insert

trLAToC27AC30A (Lat Mouse)

Tags

ER translocation signal - mEos3.2

Expression

Mammalian

Mutation

C27A, C30A

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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HA-BAP1-C91W-GFP

Plasmid

#78913

Purpose

Mammalian expression of BAP1 with C91W and an EGFP fusion

Depositor

Michael Hendzel

Article

Ismail et al Cancer Res. 2014 Aug 15;74(16):4282-94.

Insert

BAP1 (BAP1 Human)

Tags

EGFP and HA

Expression

Mammalian

Mutation

C91W

Promoter

CMV

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
pSECC-sgLKB1-A

Plasmid

#138663

Purpose

Expresses a mouse LKB1-targeting sgRNA, Cas9, and Cre-recombinase

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgLKB1 mouse (Stk11 Mouse)

Use

Lentiviral

Promoter

hU6

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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[pSK483] pLJC5 Flag-Depdc5(P)

Plasmid

#109342

Purpose

Lenti-viral expression of Flag-tagged Depdc5 mutant P

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

Insert

Depdc5 (DEPDC5 Human)

Use

Lentiviral

Tags

FLAG

Mutation

YDLLP(775-779)GSGSG

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-MTOR-D2512H

Plasmid

#69017

Purpose

activating MTOR mutation

Depositor

David Sabatini

Article

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Insert

MTOR-D2512H (MTOR Human)

Tags

FLAG

Expression

Mammalian

Mutation

change Aspartate 2512 to Histidine

Available Since

Dec. 16, 2015

Availability

Academic Institutions and Nonprofits only
pLenti-Puro-SIK2

Plasmid

#138696

Purpose

Expresses a human SIK2-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgSIK2 human (SIK2 Human)

Use

Lentiviral

Promoter

hU6

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-Puro-SIK3

Plasmid

#138697

Purpose

Expresses a human SIK3-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgSIK3 human (SIK3 Human)

Use

Lentiviral

Promoter

hU6

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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Nup98-pDONR221

Plasmid

#124241

Purpose

Gateway entry clone of Drosophila Nup98-96 cDNA.

Depositor

Peter Vilmos

Article

Kristo et al Biochim Biophys Acta Mol Cell Res. 2017

Insert

Drosophila Nup98-96 (Nup98-96 Fly)

Expression

Insect

Available Since

April 24, 2019

Availability

Academic Institutions and Nonprofits only

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B*07:05:01

Plasmid

#203268

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

B*07:05:01 (HLA-B Human)

Expression

Mammalian

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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[pSK496] pLJC5 HA-Depdc5(AB)

Plasmid

#109346

Purpose

Lenti-viral expression of HA-tagged Depdc5 mutant AB

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

Insert

Depdc5 (DEPDC5 Human)

Use

Lentiviral

Tags

HA

Mutation

DIYGD(185-189)GSGSG;EQPLH(371-375)GSGSG

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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