We narrowed to 15,991 results for: LEA

1. pcDNA3.1-hRIPK1-Flag

   Plasmid

   #112487

   Purpose

   Mammalian expression of Flag-hRIPK1. Please note Flag tag is N-terminal.

   Depositor

   Junying Yuan

   Insert

   RIPK1 (RIPK1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET His6 Mocr TEV LIC cloning vector (1O)

   Plasmid

   #29658

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   6xHis, Mocr, and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available Since

   June 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOpen-T7gene5

   Plasmid

   #165534

   Purpose

   DNA Pol with high fidelity and rapid extension rate (useful in copying long stretches of DNA); Has strong 3'-5' exonuclease.

   Depositor

   Jennifer Molloy, FreeGenes Project, Drew Endy

   Insert

   T7 DNA Polymerase (unmodified)
   

   Use

   Synthetic Biology

   Available Since

   Aug. 5, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. MZB174

   Plasmid

   #228487

   Purpose

   10xHis-SUMO (Smt3) fused, HRV-3C cleavable, GST-tag for rapid cloning/DNA assembly of pulldown targets via NotI restriction site for E. coli based protein expression

   Depositor

   Richard Baker

   Type

   Empty backbone

   Tags

   10xHis-SUMO(Smt3)-GST-HRV

   Expression

   Bacterial

   Promoter

   T7 Promoter

   Available Since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET His6 StrepII TEV LIC cloning vector (2HR-T)

   Plasmid

   #29718

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   6xHis, Strep Ii, and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET Biotin His6 mOrange LIC cloning vector (H6-mOrange)

   Plasmid

   #29723

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   6xHis, AviTag (biotin), TEV cleavage site, and mO…

   Expression

   Bacterial

   Available Since

   June 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGTvL1-SGC

   Plasmid

   #39188

   Purpose

   SGC Empty backbone for bacterial expression

   Depositor

   Nicola Burgess-Brown

   Type

   Empty backbone

   Tags

   GST and TEV cleavage site

   Expression

   Bacterial

   Promoter

   Tac (lactose/IPTG inducible)

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pET Biotin His6 mCitrine LIC cloning vector (H6-mCitrine)

   Plasmid

   #29724

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   6xHis, AviTag (biotin), TEV cleavage site, and mC…

   Expression

   Bacterial

   Available Since

   June 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. GST-TRIM24+TEV

   Plasmid

   #28147

   Depositor

   Michelle Barton

   Insert

   TRIM24 (TRIM24 Human)
   

   Tags

   GST and TEV cleavage site

   Expression

   Mammalian

   Available Since

   June 6, 2011

   Availability

   Academic Institutions and Nonprofits only

10. Kaede-Nucleus-9

    Plasmid

    #57319

    Purpose

    Localization: Nucleus, Excitation: 508 / 572, Emission: 518 / 580

    Depositor

    Michael Davidson

    Insert

    SV40 T-Antigen Nuclear Localization Signal (NLS)
    

    Tags

    Kaede-NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pET Biotin His6 FlAsH LIC cloning vector (H6-FlAsH)

    Plasmid

    #30184

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    6xHis, AviTag (biotin), FlAsH, and TEV cleavage s…

    Expression

    Bacterial

    Available Since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. tdEos-Nucleus-7

    Plasmid

    #57650

    Purpose

    Localization: Nucleus, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Insert

    SV40 T-Antigen Nuclear Localization Signal (NLS)
    

    Tags

    tdEos-NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

13. pET MBP mOrange LIC cloning vector (MBP-mOrange)

    Plasmid

    #29748

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    6xHis, AviTag (biotin), MBP, TEV cleavage site, a…

    Expression

    Bacterial

    Available Since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAS1-his-24::tagBFP2

    Plasmid

    #124321

    Purpose

    Expressing Nuclear Localized tagBFP2

    Depositor

    Andrew Leifer

    Insert

    tagBFP2 (his-24 Nematode)
    

    Expression

    Worm

    Available Since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. C83m

    Plasmid

    #120994

    Purpose

    MoClo golden gate assembly CD part for pir (Initiation factor required for replication of R6K origin plasmids). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    pir protein required for R6K ori
    

    Use

    Synthetic Biology

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX330-Flag-eSpCas9-D10A

    Plasmid

    #80449

    Purpose

    enhanced SpCas9 mammalian expression vector

    Depositor

    Ervin Welker

    Insert

    ehSpCas9_D10A
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX330-Flag-eSpCas9-H840A

    Plasmid

    #80454

    Purpose

    enhanced SpCas9 mammalian expression vector

    Depositor

    Ervin Welker

    Insert

    ehSpCas9_H840A
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAS1-his-24::tagRFP

    Plasmid

    #124329

    Purpose

    Expressing Nuclear Localized tagRFP

    Depositor

    Andrew Leifer

    Insert

    tagRFP (his-24 Nematode)
    

    Expression

    Worm

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. T16m

    Plasmid

    #121035

    Purpose

    MoClo golden gate assembly DE part for L3S2P55 (256x-strength synthetic terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    L3S2P55 (synthetic Voigt terminator)
    

    Use

    Synthetic Biology

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. PP-CDPK1 (3Q5I)

    Plasmid

    #28280

    Depositor

    Cheryl Arrowsmith

    Insert

    PP-CDPK1 (PB000158.03.0 Plasmodium berghei)
    

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Available Since

    Aug. 8, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits