We narrowed to 166,303 results for: addgene

1. pAAV-hsyn-GRAB_ATP1.0mut

   Plasmid

   #167578

   Purpose

   Express the ATP-insensitive control sensor GRAB_ATP1.0mut in neurons

   Depositor

   Yulong Li

   Article

   Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

   Insert

   ATP-insensitive sensor GRAB_ATP1.0mut (control sensor) (P2RY1 Human)
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. PtPuc3_diaCas9_sgRNA

   Plasmid

   #109219

   Purpose

   Episome based vector with CRISPR/Cas9_sgRNA module for genome editing in Phaeodactylum tricornutum

   Depositor

   Per Winge

   Article

   Sharma et al Sci Rep. 2018 Sep 26;8(1):14401. doi: 10

   Inserts

   LHCF2 promoter
   diaCas9
   LHCF1 terminator
   U6 promoter
   sgRNA
   U6 3' region
   

   Use

   CRISPR and Synthetic Biology

   Promoter

   LHCF1 terminator, LHCF2 promoter, U6 3' regi…

   Available Since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. hHS1-M7A

   Plasmid

   #159170

   Purpose

   Human codon-optimized cytosolic labile heme reporter

   Depositor

   Amit Reddi

   Article

   Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

   Insert

   hHS1-M7A
   

   Expression

   Mammalian

   Mutation

   Mutated Met 7 of the cyt b562 module of hHS1 to A…

   Promoter

   CMV

   Available Since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPS

   Plasmid

   #85801

   Purpose

   Backbone for pooled cloning of Perturb-seq libraries

   Depositor

   Aviv Regev

   Article

   Dixit et al Cell. 2016 Dec 15;167(7):1853-1866.e17.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   March 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pICSL11059

   Plasmid

   #68263

   Purpose

   Level 1 Golden Gate Cassette: Plant hygromycin (with intron) resistance cassette

   Depositor

   Nicola Patron

   Article

   Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

   Insert

   Promoter:35s + 5'UTR:Omega + CDS:hptIIwintron + 3'UTR/terminator:35s
   

   Use

   Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. qTAG-C-moxGFP-Blast-H2BC11

   Plasmid

   #207757

   Purpose

   Donor template for moxGFP-2A-Blast insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H2BC11 Homology Arms flanking a moxGFP-Blast Cassette (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Available Since

   Nov. 16, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pAV-CAG-Sun1-GFP-WPRE-BC2-pA

   Plasmid

   #220754

   Purpose

   Expression of Sun1GFP for nuclei isolation and Projection-TAG BC for multiplex projection tracing

   Depositor

   Vijay Samineni

   Article

   Yang et al Nat Commun. 2025 Jul 1;16(1):5557. doi:

   Insert

   Sun1-GFP-WPRE-BC2
   

   Use

   AAV

   Promoter

   CAG

   Available Since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAV-CAG-Sun1-GFP-WPRE-BC1-pA

   Plasmid

   #220753

   Purpose

   Expression of Sun1GFP for nuclei isolation and Projection-TAG BC for multiplex projection tracing

   Depositor

   Vijay Samineni

   Article

   Yang et al Nat Commun. 2025 Jul 1;16(1):5557. doi:

   Insert

   Sun1-GFP-WPRE-BC1
   

   Use

   AAV

   Promoter

   CAG

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. MMW1578: CAG-human dLbCpf1(D832A)-NLS-3xHA

   Plasmid

   #104563

   Purpose

   Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1)

   Depositor

   Keith Joung

   Article

   Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

   Insert

   dLbCpf1 (D832A)
   

   Use

   CRISPR

   Tags

   NLS-3xHA

   Expression

   Mammalian

   Mutation

   D832A

   Promoter

   CAG

   Available Since

   Jan. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. MAC_N_MED13

    Plasmid

    #111689

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    MED13 (MED13 Human)
    

    Expression

    Mammalian

    Available Since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLEX307 ASC

    Plasmid

    #163977

    Purpose

    Mammalian expression vector encoding ASC (for example, to assess ASC-speck formation or inflammasome signalling assays)

    Depositor

    Daniel Bachovchin

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    ASC (PYCARD Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. ADAMTS5_pCSdest

    Plasmid

    #53819

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    ADAMTS5 (ADAMTS5 Human)
    

    Expression

    Mammalian

    Promoter

    SP6 and CMV

    Available Since

    March 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pLKO human shRNA 2 DEPTOR

    Plasmid

    #21336

    Depositor

    David Sabatini

    Article

    Peterson et al Cell. 2009 May 29;137(5):873-86. doi: 10

    Insert

    DEPTOR shRNA 2 (DEPTOR Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

14. MMLV-RT(dRH) for Split-PE (pEH50)

    Plasmid

    #190106

    Purpose

    Expresses MMLV-RT (from PE2) with truncated RNAse H domain (dRH) for Split-PE use

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

    Insert

    MMLV-RT(delta RNAseH)
    

    Tags

    bpNLS

    Expression

    Mammalian

    Mutation

    mutations from RT in PE2 and truncation of RNAse …

    Promoter

    CMV

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV-EF1a-mTagBFP2-2A-Puro_hU6-DJ-DR36-BsmBI

    Plasmid

    #226009

    Purpose

    DjCas13d guide RNA cloning backbone (lenti)

    Depositor

    Silvana Konermann

    Article

    Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. 426Gal-FUS

    Plasmid

    #29591

    Depositor

    Aaron Gitler

    Article

    Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

    Insert

    FUS (FUS Human)
    

    Expression

    Yeast

    Available Since

    July 1, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pAV-CAG-Sun1-GFP-WPRE-BC6-pA

    Plasmid

    #220758

    Purpose

    Expression of Sun1GFP for nuclei isolation and Projection-TAG BC for multiplex projection tracing

    Depositor

    Vijay Samineni

    Article

    Yang et al Nat Commun. 2025 Jul 1;16(1):5557. doi:

    Insert

    Sun1-GFP-WPRE-BC6
    

    Use

    AAV

    Promoter

    CAG

    Available Since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRR-AR

    Plasmid

    #73045

    Purpose

    Constitutively expresses the androgen receptor, which is required for the activation of the promoter in pLAREG (Addgene plasmid #73045)

    Depositor

    David McMillen

    Article

    Mazumder et al Nucleic Acids Res. 2014 Aug;42(14):9514-

    Insert

    Androgen receptor (AR Human)
    

    Expression

    Yeast

    Mutation

    please see depositor comments below

    Promoter

    glyceraldehyde phosphate dehydrogenase (GPD)

    Available Since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. qTAG-C-mStayGold-Puro-PXN

    Plasmid

    #227317

    Purpose

    Donor template for mStayGold-2A-Puro insertion into the C-terminus of the PXN locus. For focal adhesion visualization. To be co-transfected with sgRNA plasmid px330-PITCh-PXN (Addgene #227315)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    PXN Homology Arms flanking a mStayGold-2A-Puro Cassette (PXN Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. EGFP-P2A_BAF_G47E

    Plasmid

    #101774

    Purpose

    Expresses mutant BAF (G47E) in human cells (with EGFP produced from the same transcript as expression control)

    Depositor

    Daniel Gerlich

    Article

    Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

    Insert

    BAF (BANF1) (BANF1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP-P2A

    Expression

    Mammalian

    Mutation

    G47E mutation

    Promoter

    EF1a

    Available Since

    Dec. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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